Development and characterization of microsatellite markers in Stryphnodendron adstringens (Leguminosae)

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dc.creatorGonçalves, Ariany Rosa
dc.creatorBarateli, Luciana Oliveira
dc.creatorSouza, Ueric José Borges de
dc.creatorPereira, Ana Maria Soares
dc.creatorBertoni, Bianca Waleria
dc.creatorTelles, Mariana Pires de Campos
dc.date.accessioned2023-02-02T12:33:42Z
dc.date.available2023-02-02T12:33:42Z
dc.date.issued2020-10
dc.description.abstractIn this study, we report the development and characterization of 15 new microsatellite markers for Stryphnodendron adstringens (Leguminosae) in order to support future analyses of genetic diversity in populations of this species. In screening with 48 individuals from three different populations of S. adstringens, we tested the amplification of 20 microsatellite loci, of which five are not useful for population genetic studies due to the lack of polymorphisms or amplification failures. For the final set of 15 loci, the number of alleles ranged from 2 to 15, with a total of 116 alleles. The expected heterozygosity ranged from 0.1219 to 0.8965, with an average of 0.6694 per locus. The combined probability of genetic identity (PI = 8.12 9 10-15) and paternity exclusion (Q = 0.99999) estimations showed that the loci may be useful to discriminate between individuals of S. adstringens. Initial cross-amplification tests were satisfactory in three species of the genus Stryphnodendron: S. rotundifolium, S. coriaceum and S. polyphyllum. This new set of markers will be a useful tool for population genetic studies, contributing to the knowledge about the evolutionary history of S. adstringens and, additionally, correlated species.pt_BR
dc.identifier.citationGONCALVES, Ariany Rosa; BARATELI, Luciana Oliveira; SOUZA, Ueric José Borges de; PEREIRA, Ana Maria Soares; BERTONI, Bianca Waléria; TELLES, Mariana Pires de Campos. Development and characterization of microsatellite markers in Stryphnodendron adstringens (Leguminosae). Physiology and Molecular Biology of Plants, Washington, v. 16, n. 10, p. 1-7, Oct. 2020. DOI: 10.1007/s12298-020-00876-1. Disponível em: https://www-ncbi-nlm-nih.ez49.periodicos.capes.gov.br/pmc/articles/PMC7548263/pdf/12298_2020_Article_876.pdf. Acesso em: 25 jan. 2023.pt_BR
dc.identifier.doi10.1007/s12298-020-00876-1
dc.identifier.issn0971-5894
dc.identifier.issne- 0974-0430
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/21855
dc.language.isoengpt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCerradopt_BR
dc.subjectSSR markerspt_BR
dc.subjectGenetic diversitypt_BR
dc.subjectMedicinal plantpt_BR
dc.subjectNext generation sequencingpt_BR
dc.titleDevelopment and characterization of microsatellite markers in Stryphnodendron adstringens (Leguminosae)pt_BR
dc.typeArtigopt_BR

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