Selection and optimization of extracellular lipase production using agro-industrial waste
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Data
2014-01
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Resumo
The aim of this study was to isolate and select lipase-producing microorganisms originated from
different substrates, as well as to optimize the production of microbial lipase by submerged
fermentation under different nutrient conditions. Of the 40 microorganisms isolated, 39 showed a halo
around the colonies and 4 were selected (Burkholderia, Bacillus sp., Penicillium lanosum and
Corynebac glutamyl), where strains showing lipolytic halo Radius (R) / colony radius (r) ratio greater
than 2.0 were selected. The results of submerged fermentation expressed as enzymatic activity revealed
that the genera of microorganisms significantly influenced the enzymatic reaction, and lipase obtained
from Burkholderia cepacia was the most promising, with activity of 0.0058 U.mL -1 . It was also observed
in the optimization step of lipase production that the sodium nitrate content (NaNO 3 ) had a positive
effect on enzyme production, and its increase was indicative of higher enzymatic activity. The addition
of sources of organic nitrogen (corn steep liquor, p = 0.2398), carbon (soybean oil, p = 0.3379),
magnesium MgSO 4 .7H 2 O (p = 0.4189) and potassium KH 2 PO 4 (p = 0.8562) had no significant effects on
the lipase production and could result in decreased production of extracellular lipases.
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Burkholderia cepacia, Residue, Hydrolytic enzymes, Submerged fermentation
Citação
BUENO, Pabline Rafaella Mello; OLIVEIRA, Tatianne Ferreira de; CALIARI, Márcio; CASTIGLIONI, Gabriel Luis; SOARES JÚNIOR, Márcio Soares. Selection and optimization of extracellular lipase production using agro-industrial waste. African Journal of Biotechnology, Lagos, v. 13, n. 4, p. 566-573, Jan. 2014.