The exoproteome profiles of three Staphylococcus saprophyticus strains reveal diversity in protein secretion contents
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2018
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Staphylococcus saprophyticus is a gram-positive microorganism responsible for urinary tract infections (UTIs).
Although some virulence factors are characterized, such as urease, autolysins, adhesins and hemagglutinins,
large-scale proteomic studies have not been performed within this species. We performed the characterization of
the exoproteome from three S. saprophyticus strains: the reference strain ATCC 15,305, a non-capsular strain
7108 and the 9325 strain containing a thick capsule which were cultured in BHI medium and culture super natants were analysed by using mass spectrometry approach. We observed a core of 72 secreted proteins. In
addition, it was possible to detect diversity in the protein profiles of the exoproteomes. Interestingly, strain 7108
presented no secretion of three antigenic proteins, including the classical SsaA antigen. In addition, the level of
antigenic proteins secreted by strain 9325 was higher than in ATCC 15,305. This result was confirmed by
Western blot analysis using anti-SsaA polyclonal antibodies, and no production/ secretion of SsaA was detected
in strain 7108. Transcriptional data shows that 7108 strain produces transcripts encoding SsaA, suggesting post transcriptional regulation occurs in this strain. Moreover, when compared with the other strains that were
analyzed, it was possible to detect higher levels of proteases secreted by strain 7108 and higher levels of anti genic proteins and transglycosylases secreted by 9325 strain. The results reveal diversity in protein secretion
among strains. This research is an important first step towards understanding the variability in S. saprophyticus
exoproteome profile and could be significant in explaining differences among strains.
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Staphylococcus saprophyticus, Exoproteome, Diversity, Proteases, Antigenic proteins, SsaA secretion
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OLIVEIRA, Andrea Santana de et al. The exoproteome profiles of three Staphylococcus saprophyticus strains reveal diversity in protein secretion contents. Microbiological Research, Amsterdam, v. 216, p. 85-96, 2018. DOI: 10.1016/j.micres.2018.08.008. Disponível em: https://www.sciencedirect.com/science/article/pii/S094450131830541X?via%3Dihub. Acesso em: 25 nov. 2024.