The exoproteome profiles of three Staphylococcus saprophyticus strains reveal diversity in protein secretion contents

dc.creatorOliveira, Andréa Santana de
dc.creatorRosa, Isabella Inês Rodrigues
dc.creatorNovaes, Evandro
dc.creatorOliveira, Lucas Silva de
dc.creatorBaeza, Lilian Cristiane
dc.creatorBorges, Clayton Luiz
dc.creatorMarlinghaus, Lennart
dc.creatorSoares, Célia Maria de Almeida
dc.creatorMarval, Marcia Giambiagi de
dc.creatorRocha, Juliana Alves Parente
dc.date.accessioned2024-11-28T15:10:18Z
dc.date.available2024-11-28T15:10:18Z
dc.date.issued2018
dc.description.abstractStaphylococcus saprophyticus is a gram-positive microorganism responsible for urinary tract infections (UTIs). Although some virulence factors are characterized, such as urease, autolysins, adhesins and hemagglutinins, large-scale proteomic studies have not been performed within this species. We performed the characterization of the exoproteome from three S. saprophyticus strains: the reference strain ATCC 15,305, a non-capsular strain 7108 and the 9325 strain containing a thick capsule which were cultured in BHI medium and culture super natants were analysed by using mass spectrometry approach. We observed a core of 72 secreted proteins. In addition, it was possible to detect diversity in the protein profiles of the exoproteomes. Interestingly, strain 7108 presented no secretion of three antigenic proteins, including the classical SsaA antigen. In addition, the level of antigenic proteins secreted by strain 9325 was higher than in ATCC 15,305. This result was confirmed by Western blot analysis using anti-SsaA polyclonal antibodies, and no production/ secretion of SsaA was detected in strain 7108. Transcriptional data shows that 7108 strain produces transcripts encoding SsaA, suggesting post transcriptional regulation occurs in this strain. Moreover, when compared with the other strains that were analyzed, it was possible to detect higher levels of proteases secreted by strain 7108 and higher levels of anti genic proteins and transglycosylases secreted by 9325 strain. The results reveal diversity in protein secretion among strains. This research is an important first step towards understanding the variability in S. saprophyticus exoproteome profile and could be significant in explaining differences among strains.
dc.identifier.citationOLIVEIRA, Andrea Santana de et al. The exoproteome profiles of three Staphylococcus saprophyticus strains reveal diversity in protein secretion contents. Microbiological Research, Amsterdam, v. 216, p. 85-96, 2018. DOI: 10.1016/j.micres.2018.08.008. Disponível em: https://www.sciencedirect.com/science/article/pii/S094450131830541X?via%3Dihub. Acesso em: 25 nov. 2024.
dc.identifier.doi10.1016/j.micres.2018.08.008
dc.identifier.issn0944-5013
dc.identifier.issne- 1618-0623
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/26016
dc.language.isoeng
dc.publisher.countryHolanda
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectStaphylococcus saprophyticus
dc.subjectExoproteome
dc.subjectDiversity
dc.subjectProteases
dc.subjectAntigenic proteins
dc.subjectSsaA secretion
dc.titleThe exoproteome profiles of three Staphylococcus saprophyticus strains reveal diversity in protein secretion contents
dc.typeArtigo

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