Produção de amilases pelo fungo macrophomina phaseolina
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Data
2007-06
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Editor
Marize Campos Valadares
Resumo
Amylases are a group of enzymes which act upon starch liberating several products including
dextrines. Progressively, small polymers made of glycose units are also liberated (GUPTA, et al., 2003). They are
of important biotechnological application chiefly in the pharmaceutical, textile, detergent and food industries.
Amylolitical enzymes are produced by bacteria, yeast and fungi. The most commonly used amylolitical enzymes
are the ones produced by filamentous fungi, notably those from the Aspergillus and Rhizopus genera (PANDEY, et
al., 1999 and PANDEY, et al., 2005). Macrophomina phaseolina belongs to the Fungi kingdom, Phylum
Ascomomycota, class Ascomycetes genus Macrophomina (GALL, et al., 1980). The Macrophomina phaseolina
fungus is a fitopatogenic agent that causes the common bacterial blight and is thus considered to be the one of the
main pathogens of bean seeds (CHIBA, et al., 2000). On the other hand, the fact that it is a producer of enzymes of biotechnological interest is largely unknown, making it difficult to assess the possible benefits this fungus might
bring to several industrial sectors.
This paper aims at evaluating the production of amylases by the fungus Macrophomina phaseolina in conventional
and non conventional carbon sources and, above all, at assessing the potential of M.phaseolina to produce
enzymes of biotechnological interest, thus offering another use of fungi in the production of molecules of interest to
the industry. Results show good specific activities of amylases with dextrinizing and saccarifiying activities in
carbon sources such as starch, rice flour and jackfruit seed flour. The total protein profiles in polyacrilamide gel
were analyzed, as well as amylolitical activity in polyacrilamide gel in nondenaturant conditions; in the latter,
isophorms were found in the protein source banana flour. Obtained results show that M. phaseolina is efficient in
obtaining expression of amylases from alternative carbon sources.
Descrição
Palavras-chave
Enzimologia, Fungos filamentosos, Diastases, Enzymolgy, Diastase, Filamentous fungi
Citação
FERNANDES, Lílian Patricia; ULHOA, Cirano José; ASQUIERI, Eduardo Ramirez; MONTEIRO, Valdirene Neves. Produção de amilases pelo fungo macrophomina phaseolina. Revista Eletrônica de Farmácia, Goiânia, v. 7, n. 1, p. 86-94, jan./jun. 2007. Disponível em: <https://revistas.ufg.br/REF/article/view/2120/2067>.