Enterobactérias isoladas de baratas (Periplaneta americana) capturadas em um hospital brasileiro
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Data
2002
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Objective. To isolate and identify microorganisms from cockroaches that were
captured in a public hospital and to test the antimicrobial susceptibility of these
microorganisms.
Methods. Cockroaches were captured in the morning and at night. They were placed
in flasks rinsed with 70% alcohol, transferred to sterilized flasks, and then taken
to the laboratory. Only cockroaches captured whole and live were utilized for the
study. After being immobilized at 0 degrees C, each cockroach was placed in a test
tube with sterile saline solution (0.8%) and then homogenized. The resulting solution
was then placed in the following five culture media: MacConkey agar, nutrient broth,
brain-heart infusion agar, Sabouraud agar, and mannitol. The cultures were examined
using a stereomicroscope, and colony-forming units were counted. The disk diffusion
test was used to determine antimicrobial susceptibility.
Results. We found a 56% prevalence of enterobacteria and an 18% prevalence of
coagulase-negative staphylococci. Fifteen species of enterobacteria were identified.
The most frequent were Klebsiella pneumoniae (17%), Enterobacter aerogenes (14%), Serratia
marcescens (13%), Hafnia alvei (12%), Enterobacter gergoviae and Enterobacter cloacae
(each 9%), and Serratia spp. (6%). Both the enterobacteria and the coagulase-negative
staphylococci showed significant resistance to antimicrobials, including oxacillin.
Conclusions. The prevalence of enteropathogenic bacteria and coagulase-negative
staphylococci isolated from Periplaneta americana cockroaches in the studied hospital
reflects the weakness of the measures adopted both for vector control and for antimicrobial
use. The results show the need to implement effective health-institution
programs focusing on hygiene and the rational use of antimicrobials.
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Infecção hospitalar, Contaminação, Controle de infecções, Insetos vetores
Citação
PRADO, Marinésia A.; PIMENTA, Fabiana C.; HAYASHIDA, Miyeko; SOUZA, Paula R.; PEREIRA, Milca S.; GIR, Elucir. Enterobactérias isoladas de baratas (Periplaneta americana) capturadas em um hospital brasileiro. Pan American Journal of Public Health, Washington, v. 11, n. 2, p. 93-97, 2002.