Avaliação do padrão de amplificação de marcadores RAPD em bandos de queixada (tayassu pecari) do Parque Nacional das Emas
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Data
2003-06
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Editor
José Henrique Stringhini
Resumo
The analysis of genetic diversity within and among
natural populations of native species is a central issue to
establish conservation and management strategies, in the
context of alternative breeding programs. The objective of
this research was to evaluate amplification pattern of primers
from RAPD markers, for two groups of white-lipped peccary
(Tayassu pecari) from Emas National Park. Forty primers
were used for the amplification through polimerase chain
reaction (PCR), using DNA from 18 individuals. The
amplified fragments were analysed by horizontal
electroforese in agarose gel. Out of the 40 primers, 30 were
successfully amplified, resulting in 203 loci, with an average
of 7 loci per primer. Considering the two groups
simultaneously, about 72% of the loci were polimorphic.
The analysis of molecular variance (AMOVA) showed a
tendency for structuring the genetic variability exists in the
groups with a fST equal to 0.081 ( P = 0.07). A principal
coordinate analysis, based on Jaccard coefficients among
individuals, also showed the same trend, suggesting that
they, at least in part, form reproductive units.
Descrição
Palavras-chave
Divergência genética, Queixadas, RAPD, Tayassu pecari, Genetic divergency, White-lipped peccary, Tayassu pecari
Citação
TELLES, Mariana Pires de Campos; RESENDE, Lucileide Vilela; SOARES, Thannya Nascimento; RODRIGUES, Flávia Melo; JÁCOMO, Anah Tereza de Almeida; SILVEIRA, Leandro. Avaliação do padrão de amplificação de marcadores RAPD em bandos de queixada (tayassu pecari) do Parque Nacional das Emas. Ciência Animal Brasileira, Goiânia, v. 4, n. 1, p. 7-14, jan./jun. 2003. Disponível em: <https://www.revistas.ufg.br/vet/article/view/301/269>.