Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L.
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Data
2009
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Resumo
This study evaluates the bioactivity of the crude ethanol extract and ethyl acetate, hexane and chloroform
fractions obtained from Eugenia uniflora leaves using the hepatopancreas of Oreochromis niloticus L. as an
experimental model. The ethanol extract and fractions were administered to the fish orally with their feed.
Twenty-four hours later, the fish were sacrificed and their livers dissected, fixed in neutral formalin,
embedded in paraffin and sectioned. Histological analyses were performed using Masson’s trichrome and
Haematoxylin-Eosin. Histochemical studies were performed using Feulgen, PAS (Periodic Acid Schiff) and
PAS + salivary amylase and Sudan IV stain. The qualitative analysis of the material showed that the crude
extract and the ethyl, chloroform and hexane fractions induced vasodilation, vascular congestion and toxicity
due to the presence of eosinophilic granular cells, rodlet cells, some leukocytic infiltrate and rare focal
necroses. The Nile tilapia proved to be a satisfactory model for screening plant products.
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Fish, Vasodilation, Medicinal plants
Citação
FIUZA, Tatiana S. et al. Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L. Biological Research, Santiago, v. 42, n. 4, p. 401-414, 2009.