Ajustes cardiovasculares induzidos pela desidratação crônica nas fases iniciais do período pós-natal em ratos acordados

Resumo

Changes in osmolarity of the extracellular compartment may change the volume, metabolism and cellular function. As a result, many complications can be observed, among them hypertension. Thus, precise regulation of the volume and osmolality of the extracellular compartment is critical to maintaining normal body functions and survival. Previous results demonstrated that despite anesthetized rats submitted to chronic diuretic treatment showed no change in sensitivity to sodium and water, an increased arterial pressure was observed. The present study aims to assess hemodynamic parameters through furosemide-induced dehydration in anaesthetized young rats. Five days old Wistar rats divided into two groups, a control (n = 10) and a group subjected to subcutaneous treatment with the diuretic Furosemide (10mg/ kg of body weight, n = 12) were used. After treatment, the animals were anesthetized for the implantation of the polyethylene tubes, which were inserted into the artery (for records cardiovascular variables) and femoral vein through a cut inguinal one side (left / right). The cannulas were transfixed subcutaneously through the back of the animal and were exteriorized. Data were recorded after a 48h recovery of the surgical procedure, using an amplifier and software. No differences in induced intake test of water within 120 minutes were observed. With respect to mean arterial pressure and heart rate, no modification on baseline levels were observed (PAM: 99.4 ± 1.9 mmHg, control vs 102.7 ± 1 mmHg, furosemide; FC: 362.5 ± 5.2 bpm, control vs 364.9 ± 7 bpm, furosemide). This response pattern was also obtained for SBP (124.3 ± 6 mmHg, control vs 130 ± 1.6 mmHg, furosemide) and DBP (82.2 ± 1.9 mmHg, control vs 82.1 ± 1 mmHg, furosemide). We concluded that depletion of the extracellular compartment induced by furosemide administration in young rats is not a determining factor to change the hemodynamic parameters in adulthood.

Descrição

Palavras-chave

Desequilíbrio hidroeletrolítico, Pressão aterial, Concentração osmolar, Desidratação

Citação

LOPES, Paulo Ricardo et al. Ajustes cardiovasculares induzidos pela desidratação crônica nas fases iniciais do período pós-natal em ratos acordados. UNOPAR Científica. Ciências Biológicas e da Saúde, Londrina, v. 17, n. 3, p. 148-153, 2015.