Determination of amoxicillin in human plasma using cephalexin as an internal standard and HPLC-MS/MS for the application of bioequivalence studies
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Data
2012-12
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Resumo
A method for determining amoxicillin in human plasma by ESI-LC-MS/MS and using cephalexin as an
internal standard was validated. The chromatographic separation was performed in a C18 Shim-pack
CLC-ODS(M) analytical column (150 mm x 4.6 mm i.d. x 5 μm), which provided satisfactory separation of
amoxicillin and cephalexin peaks, with analysis time of 3.1 minutes. A yield of extraction of 96.6% for
amoxicillin and 98.7% for cephalexin was obtained, limit of quantification of 90 ng mL-1, limit of
detection of 500 pg mL-1, and acceptable linearity for the analytical curve and calibration. Hence, the
results show that the present method is appropriate for application in bioequivalence studies of
amoxicillin, and it can also act as basis for the study of cephalexin.
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Amoxicillin, Cephalexin, LC-MS/MS, SPE, Bioequivalence
Citação
CARDOSO, C. R. F.; ALVES, Maria Isabel E.; ANTONIOSI FILHO, Nelson R. Determination of amoxicillin in human plasma using cephalexin as an internal standard and HPLC-MS/MS for the application of bioequivalence studies. International Research Journal of Pharmacy and Pharmacology, v. 2, n. 12, p. 299-305, Dec. 2012.