Determination of amoxicillin in human plasma using cephalexin as an internal standard and HPLC-MS/MS for the application of bioequivalence studies

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dc.creatorCardoso, Cláudio Roberto Fiuza
dc.creatorAlves, Maria Isabel Ribeiro
dc.creatorAntoniosi Filho, Nelson Roberto
dc.date.accessioned2018-04-11T12:51:34Z
dc.date.available2018-04-11T12:51:34Z
dc.date.issued2012-12
dc.description.abstractA method for determining amoxicillin in human plasma by ESI-LC-MS/MS and using cephalexin as an internal standard was validated. The chromatographic separation was performed in a C18 Shim-pack CLC-ODS(M) analytical column (150 mm x 4.6 mm i.d. x 5 μm), which provided satisfactory separation of amoxicillin and cephalexin peaks, with analysis time of 3.1 minutes. A yield of extraction of 96.6% for amoxicillin and 98.7% for cephalexin was obtained, limit of quantification of 90 ng mL-1, limit of detection of 500 pg mL-1, and acceptable linearity for the analytical curve and calibration. Hence, the results show that the present method is appropriate for application in bioequivalence studies of amoxicillin, and it can also act as basis for the study of cephalexin.pt_BR
dc.identifier.citationCARDOSO, C. R. F.; ALVES, Maria Isabel E.; ANTONIOSI FILHO, Nelson R. Determination of amoxicillin in human plasma using cephalexin as an internal standard and HPLC-MS/MS for the application of bioequivalence studies. International Research Journal of Pharmacy and Pharmacology, v. 2, n. 12, p. 299-305, Dec. 2012.pt_BR
dc.identifier.issn2251-0176
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/14448
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectAmoxicillinpt_BR
dc.subjectCephalexinpt_BR
dc.subjectLC-MS/MSpt_BR
dc.subjectSPEpt_BR
dc.subjectBioequivalencept_BR
dc.titleDetermination of amoxicillin in human plasma using cephalexin as an internal standard and HPLC-MS/MS for the application of bioequivalence studiespt_BR
dc.typeArtigopt_BR

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