Avaliação da sobrevivência in vitro de Lactococcus lactis QMF 11 em presença de sais biliares como critério de atividade probiótica

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Data

2020-08-21

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Universidade Federal de Goiás

Resumo

Lactic acid bacteria (LAB) have been extensively studied due to their probiotic potential. One important criteria for selecting probiotic cultures for human use is resistance to the harsh environmental conditions encountered during transit through the gastrointestinal tract (GIT). In order to reach their site of action, the large intestine, in adequate numbers to exert the probiotic effect. An important biological barrier in GIT are the bile salts present in the small intestine, as bile salts have the ability of disorganizing the cell membrane structure, thus causing damage to the genetic material of microorganisms. The objective of this work was to evaluate the survival of Lactococcus lactis QMF 11, a bacteriocinogenic strain derived from a dairy product, against bile salts as an initial indicator of probiotic activity. For that, L. lactis QMF 11 was grown in MRS broth in pH 8,0 in the presence of 0,3% and 0,5% (w/v) of bile salts. Colony counts were performed at times zero, 90 and 180 min. The results indicated that L. lactis QMF 11 remained viable throughout the trial, in numbers greater than 7.58 Log CFU/g, with a survival rate at the end of the study of 100 and 88%, respectively, in the presence of 0,3 and 0,5% bile salts. These results suggest that L. lactis QMF 11 may be a promising microorganism for use as a probiotic culture.

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Palavras-chave

Bactérias ácido láticas, Lactococcus lactis, Probiótico, Sais biliares, Lactic acid bacteria, Lactococcus lactis, Probiotic, Bile salts

Citação

SILVA, Cynthia Costa. Avaliação da sobrevivência in vitro de Lactococcus lactis QMF 11 em presença de sais biliares como critério de atividade probiótica. 35 f. Trabalho de Conclusão de Curso (Graduação) - Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, 2020.