Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology

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dc.creatorRodrigues, Roberta da Silva Bussamara
dc.creatorDall' Agnol, Luciane
dc.creatorSchrank, Augusto
dc.creatorFernandes, Kátia Flávia
dc.creatorVainstein, Marilene Henning
dc.date.accessioned2018-10-03T14:50:18Z
dc.date.available2018-10-03T14:50:18Z
dc.date.issued2012
dc.description.abstractThis study aimed to develop an optimal continuous process for lipase immobilization in a bed reactor in order to investigate the possibility of large-scale production. An extracellular lipase of Pseudozyma hubeiensis (strain HB85A) was immobilized by adsorption onto a polystyrene-divinylbenzene support. Furthermore, response surface methodology (RSM) was employed to optimize enzyme immobilization and evaluate the optimum temperature and pH for free and immobilized enzyme. The optimal immobilization conditions observed were 150 min incubation time, pH 4.76, and an enzyme/support ratio of 1282 U/g support. Optimal activity temperature for free and immobilized enzyme was found to be 68 ◦ C and 52 ◦ C, respectively. Optimal activity pH for free and immobilized lipase was pH 4.6 and 6.0, respectively. Lipase immobilization resulted in improved enzyme stability in the presence of nonionic detergents, at high temperatures, at acidic and neutral pH, and at high concentrations of organic solvents such as 2-propanol, methanol, and acetone.pt_BR
dc.identifier.citationBUSSAMARA, Roberta et al. Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology. Enzyme Reseach, London, v. 2012, p. 1-12, 2012.pt_BR
dc.identifier.doi10.1155/2012/329178
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/16112
dc.language.isoengpt_BR
dc.publisher.countryGra-bretanhapt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.titleOptimal conditions for continuous immobilization of Pseudozyma hubeiensis (strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodologypt_BR
dc.typeArtigopt_BR

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