Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology
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2012
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This study aimed to develop an optimal continuous process for lipase immobilization in a bed reactor in order to investigate
the possibility of large-scale production. An extracellular lipase of Pseudozyma hubeiensis (strain HB85A) was immobilized by
adsorption onto a polystyrene-divinylbenzene support. Furthermore, response surface methodology (RSM) was employed to
optimize enzyme immobilization and evaluate the optimum temperature and pH for free and immobilized enzyme. The optimal
immobilization conditions observed were 150 min incubation time, pH 4.76, and an enzyme/support ratio of 1282 U/g support.
Optimal activity temperature for free and immobilized enzyme was found to be 68 ◦ C and 52 ◦ C, respectively. Optimal activity pH
for free and immobilized lipase was pH 4.6 and 6.0, respectively. Lipase immobilization resulted in improved enzyme stability in
the presence of nonionic detergents, at high temperatures, at acidic and neutral pH, and at high concentrations of organic solvents
such as 2-propanol, methanol, and acetone.
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BUSSAMARA, Roberta et al. Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology. Enzyme Reseach, London, v. 2012, p. 1-12, 2012.