Radiolabed total parasite DNA probe specifically detects Trypanosoma cruzi in mammalian blood
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1988-03
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A DNA-DNA hybridization procedure is described in which radiolabeled total parasite DNA is used to detect
Trypanosoma cruzi spotted directly onto nylon membrane. Under conditions that favor hybridization of
repetitive DNA sequences, the radiolabeled total DNA was able to detect as few as five parasites spotted onto
nylon membrane. Trypanosomes were detectable in blood from mice with T. cruzi parasitemias spotted directly
onto membranes. Ali 10 strains of T. cruzi examined, which came from different areas of the United States and
South America, were readily detected with a total DNA probe from any 1 strain of the parasite. No signals were
detected with up to 20,000 Trypanosoma rangeli, T. brucei, Crithidia fasciculata, or Leishmania parasites or
with normal mouse blood or mammalian DNA. The hybridization method is sensitive, specific, rapid, and
inexpensive and is potentially applicable to the detection of other parasites.
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ASHALL, Frank; YIP-CHUCK, Deborah A. M.; LUQUETTI, Alejandro O.; MILES, Michael A. Radiolabed total parasite DNA probe specifically detects Trypanosoma cruzi in mammalian blood. Journal of Clinical Microbiology, Washington, v. 26, n. 3, p. 576-578, Mar. 1988.