On-line monitoring of biomass concentration based on a capacitance sensor: assessing the methodology for different bacteria and yeast high cell density fed-batch cultures
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Data
2015-12
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Resumo
The performance of an in-situ capacitance sensor for on-line monitoring of biomass concentration
was evaluated for some of the most important microorganisms in the biotechnology industry: Escherichia
coli, Saccharomyces cerevisiae, Pichia pastoris and Bacillus megaterium. A total of 33 batch and fed-batch
cultures were carried out in a bench-scale bioreactor and biomass formation trends were followed by
dielectric measurements during the growth phase as well as the induction phase, for 5 recombinant E. coli
strains. Permittivity measurements and viable cellular concentrations presented a linear correlation for all the
studied conditions. In addition, the permittivity signal was further used for inference of the cellular growth
rate. The estimated specific growth rates mirrored the main trends of the metabolic states of the different cells
and they can be further used for setting-up control strategies in fed-batch cultures.
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Capacitance probe, Specific growth rate, E. coli, P. pastori, S. cerevisiae, B. megaterium
Citação
HORTA, A. C. L. et al. On-line monitoring of biomass concentration based on a capacitance sensor: assessing the methodology for different bacteria and yeast high cell density fed-batch cultures. Brazilian Journal of Chemical Engineering, São Paulo, v. 32, n. 4, p. 821-829, 2015.