On-line monitoring of biomass concentration based on a capacitance sensor: assessing the methodology for different bacteria and yeast high cell density fed-batch cultures

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dc.creatorHorta, Antonio Carlos Luperni
dc.creatorSilva, Adilson José da
dc.creatorSargo, Cíntia Regina
dc.creatorMontano, Inti Doraci Cavalcanti
dc.creatorGaleano Suarez, Carlos Alberto
dc.creatorVélez Escallón, Ana María
dc.creatorSantos, M. P.
dc.creatorGonçalves, Viviane Maimoni
dc.creatorGiordano, Roberto de Campos
dc.creatorZangirolami, Teresa Cristina
dc.date.accessioned2022-07-05T14:05:20Z
dc.date.available2022-07-05T14:05:20Z
dc.date.issued2015-12
dc.description.abstractThe performance of an in-situ capacitance sensor for on-line monitoring of biomass concentration was evaluated for some of the most important microorganisms in the biotechnology industry: Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris and Bacillus megaterium. A total of 33 batch and fed-batch cultures were carried out in a bench-scale bioreactor and biomass formation trends were followed by dielectric measurements during the growth phase as well as the induction phase, for 5 recombinant E. coli strains. Permittivity measurements and viable cellular concentrations presented a linear correlation for all the studied conditions. In addition, the permittivity signal was further used for inference of the cellular growth rate. The estimated specific growth rates mirrored the main trends of the metabolic states of the different cells and they can be further used for setting-up control strategies in fed-batch cultures.pt_BR
dc.identifier.citationHORTA, A. C. L. et al. On-line monitoring of biomass concentration based on a capacitance sensor: assessing the methodology for different bacteria and yeast high cell density fed-batch cultures. Brazilian Journal of Chemical Engineering, São Paulo, v. 32, n. 4, p. 821-829, 2015.pt_BR
dc.identifier.doihttps://doi.org/10.1590/0104-6632.20150324s00003534
dc.identifier.issn0104-6632
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/20922
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Química - IQ (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCapacitance probept_BR
dc.subjectSpecific growth ratept_BR
dc.subjectE. colipt_BR
dc.subjectP. pastoript_BR
dc.subjectS. cerevisiaept_BR
dc.subjectB. megateriumpt_BR
dc.titleOn-line monitoring of biomass concentration based on a capacitance sensor: assessing the methodology for different bacteria and yeast high cell density fed-batch culturespt_BR
dc.typeArtigopt_BR

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