Suscetibilidade do E. faecalis e S.aureus a vários antimicrobianos

Abstract

Objective: It was assess the antibiotic susceptibility of E. fae- calis and S. aureus by using agar diffusion test. Material and Me- thods: For the experiment the following antibiotics were used: amoxicillin+clavulanic acid; amoxicillin; azithromycin, cephale- xin, ciprofloxacin, erythromycin, clindamycin, penicillin G, oxa- cillin and vancomycin. Twenty Petri plates with 20 mL of BHIA inoculated with 0.1 mL of bacterial infusion were sowed by steri- lized swabs. A hundred antibiotic discs were placed on BHIA pla- tes (n=10, for each group). The plates were maintained for 1 hour at room temperature and then incubated at 37oC for 48 hours. After that, the diameters of bacterial inhibition were measured, indi- cating the efficacy or not efficacy of the antibiotics, with the use of a digital caliper. Results: Among the products tested, the oxacillin did not promote inhibition zone only for E. faecalis. The amoxy- cillin associated with clavulanic acid, amoxicillin and penicillin G showed activity mainly against S. aureus. Conclusion: The most antimicrobial agents evaluated demonstrated effectiveness on microbial indicators, with average values of the inhibition zone between 15 and 35 millimeters, except oxacillin for the E. faecalis.