DNA extraction from human bone: comparison of magnetic bead and silica column techniques
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Data
2014
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Resumo
Objective: The present study aimed the comparison between the
automated magnetic bead and silica column techniques for DNA
extraction of human bones. Material and methods: Both techniques
were performed on 25 human femur bones evaluating: I) the
amount of extracted genetic material; II) the amount of amplified
profiles; and III) the necessary time range to perform the techniques.
Results: The automated magnetic bead technique recovered
larger amount of DNA in 88% of the studied bone sample in comparison
with the silica column technique. The automated magnetic
bead technique also achieved a high level of amplifications (9/16 loci) in 68% of the sample, while the silica column technique reached
equal level of amplifications only in 36% of the sample. The
time range elapsed for performing the automated magnetic bead
technique was approximately 3 hours for processing 12 samples,
while the silica column technique performed the same samples in
81 hours. Conclusion: Based on that, the automated magnetic bead
technique presented optimal outcomes and faster performance
compared to the silica column technique, revealing a valuable tool
for forensic DNA extraction.
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Palavras-chave
Magnetic bead, Partículas magnéticas, Coluna de sílica, Extração de DNA, Osso, Genética forense, Partículas magnéticas, Extração de DNA, Coluna de sílica, Genética forense, Osso
Citação
CANDIDO, Ian M. et al. A extraction from human bone: comparison of magnetic bead and silica column techniques. Robrac: revista odontologica do Brasil Central, Goiânia, v. 23, p. 155-158, 2014.