Modulation of fibronectin and laminin expression by Rhodium (II) citrate-coated maghemite nanoparticles in mice bearing breast tumor
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2017-12-20
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Degradation of cellular matrix is one of the important processes related to the progression of breast
cancer. Tumor cells have the ability to exhibit necessary conditions for growth and survival, promoting
degradation processes of extracellular matrix proteins, such as laminin (LN) and fibronectin (FN).
In this study, we evaluated whether treatments, based on free rhodium (II) citrate (Rh2(H2cit)4),
maghemite nanoparticles coated with citrate (Magh-cit) and maghemite nanoparticles coated with
rhodium (II) citrate (Magh-Rh2(H2cit)4), in murine metastatic breast carcinoma models can modulate
the expression of laminin and fibronectin proteins. Synthesized nanoparticles were characterized using
X-ray diffraction, transmission electron microscopy, energy dispersive spectroscopy and dynamic
light scattering. The expression of FN and LN was assessed using immunohistochemistry and western
blotting. The gene expression of FN1 and LAMA1 were evaluated using real-time PCR. The FN1 and
LAMA1 transcripts from the Magh-Rh2(H2cit)4 treated group were 95% and 94%, respectively, lower
than the control group. Significant reduction in tumor volume for animals treated with Magh-Rh2(H2cit)4
was observed, of about 83%. We witnessed statistically significant reductions of FN and LN expression
following treatment with Magh-Rh2(H2cit)4. We have demonstrated that the antitumor effects of Magh-
Rh2(H2cit)4 and Rh2(H2cit)4 regulate the expression of FN and LN in metastatic breast tumors.
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ROCHA, Márcia et al. Modulation of fibronectin and laminin expression by Rhodium (II) citrate-coated maghemite nanoparticles in mice bearing breast tumor. Scientific Reports, London, v. 7, e17904, 2017. DOI: 10.1038/s41598-017-18204-1. Disponível em: https://www.nature.com/articles/s41598-017-18204-1. Acesso em: 16 jun. 2023.