Genotoxic, cytotoxic, antigenotoxic, and anticytotoxic effects of sulfonamide chalcone using the ames test and the mouse bone marrow micronucleus test
Carregando...
Data
2015-09
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Chalcones present several biological activities and sulfonamide chalcone derivatives have
shown important biological applications, including antitumor activity. In this study, genotoxic,
cytotoxic, antigenotoxic, and anticytotoxic activities of the sulfonamide chalcone N-{4-
[3-(4-nitrophenyl)prop-2-enoyl]phenyl} benzenesulfonamide (CPN) were assessed using
the Salmonella typhimurium reverse mutation test (Ames test) and the mouse bone marrow
micronucleus test. The results showed that CPN caused a small increase in the number of
histidine revertant colonies in S. typhimurium strains TA98 and TA100, but not statistically
significant (p > 0.05). The antimutagenicity test showed that CPN significantly decreased
the number of His+ revertants in strain TA98 at all doses tested (p < 0.05), whereas in strain
TA100 this occurred only at doses higher than 50 μg/plate (p < 0.05). The results of the
micronucleus test indicated that CPN significantly increased the frequency of micronucleated
polychromatic erythrocytes (MNPCE) at 24 h and 48 h, revealing a genotoxic effect
of this compound. Also, a significant decrease in polychromatic/normochromatic erythrocyte
ratio (PCE/NCE) was observed at the higher doses of CPN at 24 h and 48 h (p < 0.05),
indicating its cytotoxic action. CPN co-administered with mitomycin C (MMC) significantly
decreased the frequency of MNPCE at almost all doses tested at 24 h (p < 0.05), showing
its antigenotoxic activity, and also presented a small decrease in MNPCE at 48 h (p > 0.05).
Additionally, CPN co-administered with MMC significantly increased PCE/NCE ratio at all
doses tested, demonstrating its anticytotoxic effect. In summary, CPN presented genotoxic,
cytotoxic, antigenotoxic, and anticytotoxic properties.
Descrição
Palavras-chave
Citação
SILVA, Carolina Ribeiro e et al. Genotoxic, cytotoxic, antigenotoxic, and anticytotoxic effects of sulfonamide chalcone using the ames test and the mouse bone marrow micronucleus test. Plos One, San Francisco, v. 10, e0137063, Sept. 2015.