Aislamiento, viabilidad y rendimiento de células mononucleares de médula ósea de conejos
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Data
2011
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Resumo
The aim of this study was to establish a cellular isolation protocol and evaluate
the yield and viability of bone marrow mononuclear cells in rabbits.
Thirty-two male adult New Zealand rabbits were used with 3.06 ± 0.24 Kg
mean weight. A volume of 2.0 mL bone marrow was aspirated from the
humerus of each animal. Samples were diluted in Dulbecco phosphate
buffered saline (DPBS) and submitted to density gradient using Ficoll-Ipaque
(1.077 g/mL) at a 2:1 rate. Subsequently, the material was centrifuged at
495g during 30 minutes, at 15 oC. The yield and cell viability were determined in Neubauer haemocytometer chamber, using trypan blue vital staining. A mean yield [±SD]
of 6.65 [±4.18] x106 cells/mL was obtained and the mean viability [±SD] was 92.66 [±4.35]
%. The protocol using density gradient was effective for the isolation, yield and viability of
bone marrow mononuclear cells in rabbits in order to use them in cell therapy.
Descrição
Palavras-chave
Células madre mesenquimales, Médula ósea, Aislamiento, Conejos, Mesenchymal stem cells, Bone marrow, Isolation, Rabbits
Citação
OLIVEIRA, Benito Juarez Nunes Alves de; SOUZA, Luiz Augusto de; EURIDES, Duvaldo; SILVA, Luiz Antônio Franco da; BAUNGARTEN, Letícia Binda; BUSNARDO, Camila Araújo. Aislamiento, viabilidad y rendimiento de células mononucleares de médula ósea de conejos. Acta Bioquímica Clínica Latinoamericana, La Plata, v. 45, n. 1, p. 113-118, 2011.