Intrinsic denervation of the colon is associated with a decrease of some colonic preneoplastic markers in rats treated with a chemical carcinogen
Carregando...
Data
2008
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Denervation of the colon is protective against the colon cancer; however, the mechanisms involved are unknown. We tested the
hypothesis that the denervated colonic mucosa could be less responsive to the action of the chemical carcinogen dimethylhy-
drazine (DMH). Three groups of 32 male Wistar rats were treated as follows: group 1 (G1) had the colon denervated with 0.3 mL
1.5 mM benzyldimethyltetradecylammonium (benzalkonium chloride, BAC); G2 received a single ip injection of 125 mg/kg DMH;
G3 was treated with BAC + the same dose and route of DMH. A control group (Sham, N = 32) did not receive any treatment. Each
group was subdivided into four groups according to the sacrifice time (1, 2, 6, and 12 weeks after DMH). Crypt fission index, ß-
catenin accumulated crypts, aberrant crypt foci, and cell proliferation were evaluated and analyzed by ANOVA and the Student
t-test. G3 animals presented a small number of aberrant crypt foci and low crypt fission index compared to G2 animals after 2
and 12 weeks, respectively. From the second week on, the index of ß-catenin crypt in G3 animals increased slower than in G2
animals. From the 12th week on, G2 animals presented a significant increase in cell proliferation when compared to the other
groups. Colonic denervation plays an anticarcinogenic role from early stages of colon cancer development. This finding can be
of importance for the study of the role of the enteric nervous system in the carcinogenic process
Descrição
Palavras-chave
Colon cancer, Megacolon, Dimethylhydrazine, ß-catenin, Benzalkonium chloride, Aberrant crypt foci
Citação
VESPUCIO, M. V. O. et al. Intrinsic denervation of the colon is associated with a decrease of some colonic preneoplastic markers in rats treated with a chemical carcinogen. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 41, n. 4, p. 1-7, 2008.