Serological diagnosis of Chagas' disease: a potential confirmatory assay using preserved protein antigens of Trypanosoma cruzi
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1997-07
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The diagnosis of Chagas’ disease relies mostly on data provided by immunologic tests, but inconclusive
results often require elucidation, especially in blood banks. When six different types of Trypanosoma cruzi
epimastigote antigens were studied by an immunoblotting assay (IBA), a preserved protein antigen (Ag PP)
was found to present the most interesting immunochemical features because of its high reactivity with anti-T.
cruzi antibodies. Thus, the IBA with Ag PP (PP IBA) was assessed with panels of coded and noncoded serum
samples prepared in different laboratories, including the Brazilian Reference Laboratory for Chagas’ Disease.
It was found that serum samples from patients proved (clinically, eletrocardiographically, serologically, and
epidemiologically) to have Chagas’ disease consistently recognized 12 bands (140, 100, 85, 78, 59, 57, 46, 35, 27,
23, 20, and 18 kDa) of Ag PP. In contrast, sera from nonchagasic patients, including patients with mucocutaneous
leishmaniasis, were negative or reacted weakly, and one serum sample did not have more than five
different bands. These bands were 78, 57, 46, 35, 27, 23, 20, or 18 kDa. A criterion was adopted to interpret the
results obtained in the PP IBA. The criterion considered positive a serum sample recognizing all 12 bands and
considered negative a serum sample that did not recognize any of the bands except the eight nonspecific bands
mentioned above. The PP IBA indicated maximum sensitivity and specificity as well as high positive and
negative predictive values. The data demonstrate that the PP IBA discriminates chagasic from nonchagasic
infections and seems to be applicable as a confirmatory assay for elucidating inconclusive results obtained by
standard serology.
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MENDES, Rodolfo P.; SHIMIZU, Sumie-Hoshino; SILVA, Ana M. Moura da; MOTA, Ivan; HEREDIA, Himmel A. G.; LUQUETTI, Alejandro O.; LESER, Paulo G.; Serological diagnosis of Chagas' disease: a potential confirmatory assay using preserved protein antigens of Trypanosoma cruzi. Journal of Clinical Microbiology, Washington, v. 35, n. 7, p. 1829-1834, July 1997.