Serological diagnosis of Chagas' disease: a potential confirmatory assay using preserved protein antigens of Trypanosoma cruzi

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dc.creatorMendes, Rodolfo Pereira
dc.creatorShimizu, Sumie Hoshino
dc.creatorSilva, Ana Maria Moura da
dc.creatorMota, Ivan
dc.creatorHeredia, Himmel Amador Gusman
dc.creatorOstermayer, Alejandro Luquetti
dc.creatorLeser, Paulo Guilherme
dc.date.accessioned2018-04-27T14:34:41Z
dc.date.available2018-04-27T14:34:41Z
dc.date.issued1997-07
dc.description.abstractThe diagnosis of Chagas’ disease relies mostly on data provided by immunologic tests, but inconclusive results often require elucidation, especially in blood banks. When six different types of Trypanosoma cruzi epimastigote antigens were studied by an immunoblotting assay (IBA), a preserved protein antigen (Ag PP) was found to present the most interesting immunochemical features because of its high reactivity with anti-T. cruzi antibodies. Thus, the IBA with Ag PP (PP IBA) was assessed with panels of coded and noncoded serum samples prepared in different laboratories, including the Brazilian Reference Laboratory for Chagas’ Disease. It was found that serum samples from patients proved (clinically, eletrocardiographically, serologically, and epidemiologically) to have Chagas’ disease consistently recognized 12 bands (140, 100, 85, 78, 59, 57, 46, 35, 27, 23, 20, and 18 kDa) of Ag PP. In contrast, sera from nonchagasic patients, including patients with mucocutaneous leishmaniasis, were negative or reacted weakly, and one serum sample did not have more than five different bands. These bands were 78, 57, 46, 35, 27, 23, 20, or 18 kDa. A criterion was adopted to interpret the results obtained in the PP IBA. The criterion considered positive a serum sample recognizing all 12 bands and considered negative a serum sample that did not recognize any of the bands except the eight nonspecific bands mentioned above. The PP IBA indicated maximum sensitivity and specificity as well as high positive and negative predictive values. The data demonstrate that the PP IBA discriminates chagasic from nonchagasic infections and seems to be applicable as a confirmatory assay for elucidating inconclusive results obtained by standard serology.pt_BR
dc.identifier.citationMENDES, Rodolfo P.; SHIMIZU, Sumie-Hoshino; SILVA, Ana M. Moura da; MOTA, Ivan; HEREDIA, Himmel A. G.; LUQUETTI, Alejandro O.; LESER, Paulo G.; Serological diagnosis of Chagas' disease: a potential confirmatory assay using preserved protein antigens of Trypanosoma cruzi. Journal of Clinical Microbiology, Washington, v. 35, n. 7, p. 1829-1834, July 1997.pt_BR
dc.identifier.issn0095-1137
dc.identifier.issne- 1098-660X
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/14718
dc.language.isoengpt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.titleSerological diagnosis of Chagas' disease: a potential confirmatory assay using preserved protein antigens of Trypanosoma cruzipt_BR
dc.typeArtigopt_BR

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