Host protein biomarkers identify active tuberculosis in HIV uninfected and co-infected individuals
Data
2015
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Resumo
Biomarkers for active tuberculosis (TB) are urgently needed to improve rapid TB diagnosis. The objective of
this study was to identify serum protein expression changes associated with TB but not latent Mycobacterium
tuberculosis infection (LTBI), uninfected states, or respiratory diseases other than TB (ORD). Serum samples
from 209 HIV uninfected (HIV−) and co-infected (HIV+) individuals were studied. In the discovery phase
samples were analyzed via liquid chromatography and mass spectrometry, and in the verification phase biologically
independent samples were analyzed via a multiplex multiple reaction monitoring mass spectrometry
(MRM-MS) assay. Compared to LTBI and ORD, host proteins were significantly differentially expressed in TB,
and involved in the immune response, tissue repair, and lipid metabolism. Biomarker panels whose composition
differed according to HIV status, and consisted of 8 host proteins in HIV− individuals (CD14, SEPP1, SELL, TNXB,
LUM, PEPD, QSOX1, COMP, APOC1), or 10 host proteins in HIV+ individuals (CD14, SEPP1, PGLYRP2, PFN1, VASN,
CPN2, TAGLN2, IGFBP6), respectively, distinguished TB from ORD with excellent accuracy (AUC=0.96 for HIV−
TB, 0.95 for HIV+ TB). These results warrant validation in larger studies but provide promise that host protein
biomarkers could be the basis for a rapid, blood-based test for TB.
Descrição
Palavras-chave
Host proteins, Tuberculosis, Biomarker, Diagnostics
Citação
ACHKAR, Jacqueline M. et.al. Host protein biomarkers identify active tuberculosis in HIV uninfected and co-infected individuals. EBioMedicine, Amsterdã, v. 2, p. 1160-1168, 2015.