Anticorpos para o herpesvírus bovino 1 (BHV-1) em bovinos do estado de Goiás

Abstract

This study was conducted to detect antibodies to BHV-1 in cattle sera from some counties from State of Goiás, Brazil. Seven hundred and ninety (790) bovine serum samples were tested through a commercial enzyme immunosorbant assay (ELISA). The samples were in the majority (75.3%) from cattle with clinical signs associated to reproductive problems. The sera were collected from 90 properties including dairy, beef and mixed herds, distributed in 40 counties of Goiás. The positiveness rate for BHV-1 was 83.0%. Dairy cattle showed a higher rate than beef cattle (p<0.01). Within the properties included in this study, 96.7% presented positive animals and 97.5% of the counties presented at least one positive animal. Paired sera were obtained from 420 samples and they were also tested by serum neutralization test (SN) with one hour of virus-serum incubation period. Only 7.8% (n=33) of the samples showed seroconvertion, indicating acute infection. The samples that showed discrepant results between the ELISA and SN (n=46), were retested by SN using different virus-serum incubation periods. There was an increasing in the detection of positive samples while the incubation virus–serum increase. These results demonstrated that BHV-1 is circulating in the State of Goiás and suggests that a 24 hours virus incubation period must be used in the serum neutralization test for BHV-1 diagnosis.