Estudo de toxicidade retiniana após injeções intravítreas seriadas de infliximabe em olhos de coelhos
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Data
2011
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Resumo
Purpose: To determine retinal and choroid toxicity levels of two and three infliximab
intravitreous injections in albino rabbits by means of electroretinographic, histological
and ophthalmological clinical tests.
Methods: 12 albino rabbits were used in the study. Each eye was given two (n=10 eyes)
or three (n=10 eyes) serial intravitreous 2 mg infliximab injections dissolved in 0.06 ml of
saline, at monthly intervals. A separate group of rabbits (n=4 eyes) served as a control
group. Ninety days after the study had begun, the rabbits underwent clinical and electro-
retinographic tests, and after being enucleated, the eyes were examined for histological
changes.
Results: Slit-lamp biomicroscopy and fundoscopic examination did not reveal any
significant retinal abnormalities in the eyes injected with infliximab and control eyes or in
pre- and post-treated eyes. The histological change that was noted was the presence of
rare lymphocytes and eosinophils in the posterior vitreous of some of the rabbits subjected
to two or three injections, but it was not considered clinically significant. A severe
inflammatory reaction with vitreous exudates and ganglion cell edema in a single rabbit
was clinically significant. The electroretinographic tests showed amplitudes that were on
the average 12-13% smaller than those obtained before the treatment, however, there
were no statistically significant differences when comparing the amplitude or the implicit
time between pre- and post-treatment electroretinographic findings.
Conclusion: Two and three intravitreous 2 mg infliximab injections in rabbits at monthly
intervals did not cause any changes after a 90-day follow-up, according to histological
and electroretinographic tests and after clinical evaluation. Differently from prior studies
that have investigated potential retinotoxicity effects after single administrations, this
study investigated the possibility of retinotoxicity after multiple injections. Clinical studies
in humans should be conducted to better evaluate the safety of this drug in the treatment
of certain diseases affecting the retina and the choroid.
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Palavras-chave
Fator de necrose tumoral alfa, Animais, Injeções intravenosas, Eletrorretinografia, Coelhos, Retina, Doenças retinianas/quimioterapia, Doenças da coróide/quimioterapia, Degeneração macular, Anticorpos monoclonais/administração & dosagem, Tumor necrosis factor-alpha, Injections, intravenous, Retina, Retinal diseases/ drug therapy, Choroid diseases/drug therapy, Animals, Rabbits, Macular degeneration, Antibodies, mono- clonal/administration & dosage, Electroretinography
Citação
RASSI, Alan Ricardo et al. Estudo de toxicidade retiniana após injeções intravítreas seriadas de infliximabe em olhos de coelhos. Arquivos Brasileiros de Oftalmologia, São Paulo, v. 74, n. 5, p. 352-356, 2011.