Microbiota cérvico-vaginal durante o final de gestação e puerpério em vacas girolando

Abstract

The present study was designed to evaluate the changes in the cervico-vaginal microbiota during the period between 27 days before until 58 days after parturition in 23 Girolando cows. The cervico-vaginal mucus was collected with 14 days intervals, except the period between the day of parturition and two days after. Bacteriological culture was performed for 117 samples and 101 isolations were achieved. Negative cultures were concentrated mainly during the period before parturition (58,8%) and the final of puerperium (44,4%), but from the day or parturition until the 16th day after all of the samples were positive for bacterial cultive and isolation. The agents isolated were: Escherichia coli (27,72%), Staphylococcus coagulase negative (21,78%), Staphylococcus coagulase positive (1,98%), Streptococcus spp. (19,80%), Corynebacterium spp. (7,92%), Corynebacterium bovis (3,98%), Bacillus spp. (6,98%), Pseudomonas spp. (3,96%), Enterobacter aerogenes (1,98%), Actinomyces pyogenes (1,98%), Citrobacter freundii (0,99%) and Citrobacter amalonaticus (0,99%). Primiparous females showed more positive samples during the time of puerperium, suggesting an association between the age of the animal and the sensivity to bacterial colonization of the reproductive tract.