Biotransformaçâo do 1,8–cineol por bactérias livres e imobilizadas
Data
2005-06
Título da Revista
ISSN da Revista
Título de Volume
Editor
Marize Campos Valadares
Resumo
The essential oil of leaves of the Eucalyptus microcorys F. Muller collected in Silvânia-GO, possesses
87% of 1,8-cineole. This monoterpene is one of the main composed of the essential oils of Eucalyptus globulus Labill
and they are of great interest for the industries, mainly the perfumeries, food and pharmaceutical. The objective of this
work was the hydroxylation of 1,8-cineole for free and immobilized bacterias. The hidroxilation of the molecule of 1,8-
cineole is important for obtaining new aromas. The essential oil of Eucalyptus microcorys also presented inhibition on
the growth of Staphylococcus aureus ATCC 6538 and the hydroxylation of the molecule of 1,8-cineole can increase
this activity antibacterial. In this work it was tested the Pseudomonas putida, Azotobacter vinelandii and Pseudomonas fluorescence bacterias. However the Pseudomonas putida went to that presented a significant result and facilitated the
biotransformation of the composition 1,8-cineole. The main culture used for biotransformation (MMB) was: 0,5g of
glucose, 0,2g of (NH4)2SO4, 0,4g of KH2PO4, 0,6g of Na2HPO4, 0,2g of MgSO4, amount lines of Fe, B, Mn, Zn, Cu and
Mo in 100 mL of distilled water. After 60-65 hours to 30oC, pH 6,5 and 180 rpm 100 μL the 1,8-cineole was added in
main MMB and was maintained at the same temperature conditions, pH and agitation, for periods of 48-120 hours. The
solvent that showed more efficient for extraction of 1,8-cineole was the ethyl acetate. The ideal revelator for the
composition 1,8-cineole at thin layer chromatography was a solution of 10 mL of H2SO4 concentrated, 45 mL ethanol,
1g of vanillin and 10 mL of distilled water. The mobile phase used at the chromatography was 1:3 of ethyl/hexano
acetate. The results of biotransformation were investigated through the technique of GC/MS. The bacteria
Pseudomonas putida got transform the substratum 1,8-cineole in two new composed: exo-2-hydroxycineole and the 1-
hydroxy-p-menth-3-one.
Descrição
Palavras-chave
1,8 - Cineol, Biotransformação, Bactérias, Imobilização, 1,8 – Cineole, Biotransformation, Bacterias, Imobilization
Citação
ALMEIDA, Ludimila P.; FERRI, Pedro Henrique; PAULA, José Realino de; SANTIAGO, Mariângela Fontes. Biotransformaçâo do 1,8–cineol por bactérias livres e imobilizadas. Revista Eletrônica de Farmácia, Goiânia, v. 2, n. 1, p. 1-5, jan./jun. 2005. Disponível em: <https://www.revistas.ufg.br/REF/article/view/1941/1873>.