Comparative in vitro and in planta analyses of extracellular enzymes secreted by the pathogenic fungus Sclerotinia sclerotiorum
Carregando...
Data
2013
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Dry bean (Phaseolus vulgaris L.) is an important
economic crop in Brazil but its yield can be significantly reduced by
white mold, a disease caused by Sclerotinia sclerotiorum (Lib.) de
Bary, a necrotrophic, highly destructive, and non-host-specific fungus.
This fungus secretes numerous cell wall-degrading enzymes such as
polygalacturonases, exo-β-1,3-glucanases, xylanases, and cellulases
that have been detected during the early stages of infection. In this
study, the activities of these enzymes were detected in all carbon sources
tested (citrus pectin, cell wall extract from P. vulgaris, carboxymethyl
cellulose, and glucose), but the highest levels were found when using
citrus pectin and cell wall extract from P. vulgaris. Regardless of the
carbon source, pH decreased throughout the culture time. During
pathogenesis in dry bean stems, increased enzyme activities were also
observed. Reverse transcriptase-polymerase chain reaction experiments
showed that the induction of polygalacturonases (sspg1, sspg3, sspg5,
sspg6, and sspg7), exo-β-1,3-glucanases, and endo-β-1-4-glucanase in
S. sclerotiorum occurred during the early stages of colonization.
Descrição
Palavras-chave
Endopolygalacturonase, White mold, Xylanases, Cellulases
Citação
OLIVEIRA, M. B.; BARBOSA, S. C.; PETROFEZA, S. Comparative in vitro and in planta analyses of extracellular enzymes secreted by the pathogenic fungus Sclerotinia sclerotiorum. Genetics and Molecular Research, Ribeirão Preto, v. 12, n. 2, p. 1796-1807, 2013.