Comparative in vitro and in planta analyses of extracellular enzymes secreted by the pathogenic fungus Sclerotinia sclerotiorum

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dc.creatorOliveira, Marília Barros
dc.creatorBarbosa, S. C.
dc.creatorSilva, Silvana Petrofeza da
dc.date.accessioned2018-10-22T12:23:31Z
dc.date.available2018-10-22T12:23:31Z
dc.date.issued2013
dc.description.abstractDry bean (Phaseolus vulgaris L.) is an important economic crop in Brazil but its yield can be significantly reduced by white mold, a disease caused by Sclerotinia sclerotiorum (Lib.) de Bary, a necrotrophic, highly destructive, and non-host-specific fungus. This fungus secretes numerous cell wall-degrading enzymes such as polygalacturonases, exo-β-1,3-glucanases, xylanases, and cellulases that have been detected during the early stages of infection. In this study, the activities of these enzymes were detected in all carbon sources tested (citrus pectin, cell wall extract from P. vulgaris, carboxymethyl cellulose, and glucose), but the highest levels were found when using citrus pectin and cell wall extract from P. vulgaris. Regardless of the carbon source, pH decreased throughout the culture time. During pathogenesis in dry bean stems, increased enzyme activities were also observed. Reverse transcriptase-polymerase chain reaction experiments showed that the induction of polygalacturonases (sspg1, sspg3, sspg5, sspg6, and sspg7), exo-β-1,3-glucanases, and endo-β-1-4-glucanase in S. sclerotiorum occurred during the early stages of colonization.pt_BR
dc.identifier.citationOLIVEIRA, M. B.; BARBOSA, S. C.; PETROFEZA, S. Comparative in vitro and in planta analyses of extracellular enzymes secreted by the pathogenic fungus Sclerotinia sclerotiorum. Genetics and Molecular Research, Ribeirão Preto, v. 12, n. 2, p. 1796-1807, 2013.pt_BR
dc.identifier.doi10.4238/2013.June.6.3.
dc.identifier.issne- 1676-5680
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/16222
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectEndopolygalacturonasept_BR
dc.subjectWhite moldpt_BR
dc.subjectXylanasespt_BR
dc.subjectCellulasespt_BR
dc.titleComparative in vitro and in planta analyses of extracellular enzymes secreted by the pathogenic fungus Sclerotinia sclerotiorumpt_BR
dc.typeArtigopt_BR

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