Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction
Carregando...
Data
2010
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Objective: The aim of this study was to detect the prevalence of selected bacterial
species in intraoral sites of patients with chronic periodontitis (CP) using multiplex
polymerase chain reaction (PCR). Methodology: Samples were collected from the tongue
dorsum, buccal mucosa, supragingival and subgingival plaque and saliva of 30 patients
with untreated CP. Multiplex PCR was used to determine prevalence rates, which were
then compared using a chi-square test. Significance level was set at p<0.05. Mean and
standard deviation values were used to evaluate variations in prevalence according to
site. Results: The prevalence of S. mutans was 70% in saliva; 60% in samples collected
from the tongue dorsum; 50% in samples collected from the buccal mucosa; 56.5% in the
supragingival plaque; and 53.5% in the subgingival plaque. The prevalence of E. faecalis
ranged from 3.5% to 13.5% in all intraoral microenvironment. The highest prevalence of P.
gingivalis was found in subgingival plaque (53.5%), and of P. intermedia in supragingival
plaque (33.5%), subgingival plaque (30%) and tongue dorsum (33.5%). The prevalence
of bacteria did not vary significantly among the intraoral sites. Conclusions: All studied
bacteria were identified in intraoral sites. S. mutans, P. gingivalis and P. intermedia had
high prevalence rates, but the prevalence of E. faecalis was low. Multiplex PCR proved to
be an adequate method for epidemiological studies.
Descrição
Palavras-chave
Chronic periodontitis, Epidemiological studies, Bacteria, DNA probes, PCR
Citação
ESTRELA, Cyntia Rodrigues de Araújo et al. Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction. Journal of Applied Oral Science, Bauru, v. 18, n. 4, p. 423, 2010.