Metil-formamida na criopreservação de sêmen ovino
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Data
2013-12
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Editor
José Henrique Stringhini
Resumo
The aim of this study was to evaluate the efficacy of
methyl-formamide in ram semen cryopreservation. The
semen pool used in this experiment was obtained by
artificial vagina collection from four mixed breed Santa
Inês rams, around four years of age. Semen collection was
performed once a week, during six weeks. Each week
corresponded to one experiment replication. The semen
pool was divided in five fractions in order to be diluted in
one of the following freezing media: (1) tris-egg yolk with
5.3% of glycerol (TG5.3G); (2) tris-egg yolk with 3% of
methyl-formamide (TG3MF); tris-egg yolk with 5% of
methyl-formamide (TG5MF); tris-egg yolk with 7% of methyl-formamide (TG7MF); tris-egg yolk with 9% of
methyl-formamide (TG9MF). Semen progressive motility,
vigor and thermoresistance were evaluated. The
treatments TG5.3G (50%) and TG3MF (38%) showed
higher progressive motility after thawing, while TG5MF
(29%), TG7MF (1%) and TG9MF (6%) showerd lower
motility. Freezing media containing methyl-formamide
were less effective in preserving spermatozoa membrane
integrity and morphology than control media. In TG7MF
and TG9MF extenders, less than 60% spermatozoa
showed normal morphology. After thermoresistance test,
semen cryopreserved in TG3MF showed vigor (2.6) and
motility (15%) statistically similar to TG5.3G media (15%
and 2.8, respectively); however, the extender TG5.3G was
more effective in preserving plasma membrane functional
integrity. In conclusion, in the experimental conditions
used, glycerol showed more cryoprotectant potential than
methyl-formamide.
Descrição
Palavras-chave
Amidas, Carneiros, Glicerol, Tris-gema, Amide, Glycerol, Ram, Tris-egg yolk
Citação
GRAÇAS, Carlos Pereira das; LIM, Alexandre In Piao Gomes; FIDELIS, Andrei Antonioni Guedes; CARDOSO, Júlio Roquete; BLUME, Hélio; MONDADORI, Rafael Gianella. Metil-formamida na criopreservação de sêmen ovino. Ciência Animal Brasileira, Goiânia, v. 14, n. 4, p. 481-487, out./dez. 2013. Disponível em: <https://www.revistas.ufg.br/vet/article/view/17835/15640>.