Sequence analysis and characterization of rolling-circle replicating plasmid pVCM01 from Salmonella enterica

Página do item simplificado
dc.creatorTroian, Ana Flavia Batista Penido
dc.creatorMendes, Paulo Vinicius da Costa
dc.creatorCampos, Ivan Torres Nicolau de
dc.creatorBataus, Luiz Artur Mendes
dc.date.accessioned2020-10-08T12:50:11Z
dc.date.available2020-10-08T12:50:11Z
dc.date.issued2013
dc.description.abstractAims: Characterization of cryptic plasmid pVCM01 (accession number JX133088) isolated from Salmonella enterica Enteritidis. Methodology and results: The complete sequence of pVCM01 was obtained. This plasmid possesses 1981 bp, with G+C content of 57% in agreement of the range of Salmonella genomic DNA. pVCM01 has a high degree of similarity to pB and pJ plasmids. It possesses six main open reading frames, only one have a very high degree of amino acid identity with protein involved in the rolling-circle-like replication (RCR). Based on the sequence similarities, pVCM01 plasmid belonged to the pC194/pUB110 rolling-circle replicating plasmid family. The Rep pVCM01 possesses the motifs: FLTLTVRN, HPHFHTL, SGDGYVKHERW, which were present in all Rep proteins. Conclusion, significance and impact of study: The small size of pVCM01 plasmid and its stability in E. coli cells, make it an attractive candidate to develop new vectors, such as cloning and/or expression vector.pt_BR
dc.identifier.citationPENIDO, Ana Flávia Batista et al. Sequence analysis and characterization of rolling-circle replicating plasmid pVCM01 from Salmonella enterica. Malaysian Journal of Microbiology, Penang, v. 9, n. 4, p. 331-334, 2013.pt_BR
dc.identifier.doi10.21161/mjm.50913
dc.identifier.issn1823-8262
dc.identifier.issne- 2231-7538
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/19143
dc.language.isoengpt_BR
dc.publisher.countryOutrospt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectSalmonella entericapt_BR
dc.subjectRep proteinpt_BR
dc.subjectRolling-circle replicating plasmidpt_BR
dc.titleSequence analysis and characterization of rolling-circle replicating plasmid pVCM01 from Salmonella entericapt_BR
dc.typeArtigopt_BR

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Artigo - Ana Flavia Batista Penido Troian - 2013.pdf
Tamanho:
271.7 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

EA - Artigos publicados em periódicos