Sequence analysis and characterization of rolling-circle replicating plasmid pVCM01 from Salmonella enterica
Carregando...
Data
2013
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Aims: Characterization of cryptic plasmid pVCM01 (accession number JX133088) isolated from Salmonella enterica
Enteritidis.
Methodology and results: The complete sequence of pVCM01 was obtained. This plasmid possesses 1981 bp, with
G+C content of 57% in agreement of the range of Salmonella genomic DNA. pVCM01 has a high degree of similarity to
pB and pJ plasmids. It possesses six main open reading frames, only one have a very high degree of amino acid identity
with protein involved in the rolling-circle-like replication (RCR). Based on the sequence similarities, pVCM01 plasmid
belonged to the pC194/pUB110 rolling-circle replicating plasmid family. The Rep pVCM01 possesses the motifs:
FLTLTVRN, HPHFHTL, SGDGYVKHERW, which were present in all Rep proteins.
Conclusion, significance and impact of study: The small size of pVCM01 plasmid and its stability in E. coli cells,
make it an attractive candidate to develop new vectors, such as cloning and/or expression vector.
Descrição
Palavras-chave
Salmonella enterica, Rep protein, Rolling-circle replicating plasmid
Citação
PENIDO, Ana Flávia Batista et al. Sequence analysis and characterization of rolling-circle replicating plasmid pVCM01 from Salmonella enterica. Malaysian Journal of Microbiology, Penang, v. 9, n. 4, p. 331-334, 2013.