Produção, caracterização e avaliação de enzimas fibrolíticas na digestibilidade da forragem de milho
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Data
2013-12
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Editor
José Henrique Stringhini
Resumo
The objectives of this research were to produce and
characterize an enzyme complex (EC), using the fungus
Humicola grisea, and evaluate its effect on true
digestibility of forage maize dry matter. We observed that
the fungus produced cellulase, -glucosidase and xylanase
enzymes. The cellulase and xylanase activities were high
at the temperature of 50° C. The optimum temperature of
β-glucosidase was between 50 and 60° C. The optimum
pH of cellulase and xylanase enzyme was 6.0. As for β-
glucosidase, the enzyme showed higher activity at pH 6.5.
Cellulase remained stable for 60 minutes at 39° C.
Xylanase and β-glucosidase maintained 99.2 and 88.2% of
their activity at 50° C for 240 minutes, respectively. The
treatments were as follows: control (10 mL of sterile
water), level 1 (2.5 mL EC), level 2 (5.0 mL EC) and level
3 (10 mL EC). In the digestibility experiment, there was
interaction between enzyme levels and time of incubation
in the rumen. The addition of 10 mL of the fibrolytic
enzymes improved the digestibility at 10.58; 12.52; 9.05
and 6.81% compared to control for 12; 24; 48 and 96
hours of incubation, respectively. The fungus Humicola
grisea is an enzyme producer that is important in ruminant
feed.
Descrição
Palavras-chave
Ankom, Bovino, Celulase, Fungo, Xilanase, Bovine, Cellulose, Fungi, Xylanase
Citação
CYSNEIROS, Cristine dos Santos Settimi; FERREIRA, Reginaldo Nassar; OLIVEIRA, Michelly Ayres; FAVORETTO, Adriano Oliveira; ARNHOLD, Emmanuel; ULHOA, Cirano José. Produção, caracterização e avaliação de enzimas fibrolíticas na digestibilidade da forragem de milho. Ciência Animal Brasileira, Goiânia, v. 14, n. 4, p. 426-435, out./dez. 2013. Disponível em: <https://www.revistas.ufg.br/vet/article/view/19491/15626>.