Doutorado em Genética e Melhoramento de Plantas (EA)
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Item Seleção de genótipos e mapeamento de QTLs para resistência à murcha de fusário em feijão carioca(Universidade Federal de Goiás, 2021-10-07) Cavalheiro, Sâmela Beutinger; Pereira, Helton Santos; http://lattes.cnpq.br/0729719587905292; Pereira, Helton Santos; Torga, Paula Pereira; Ishikawa, Francine Hiromi; Vianello, Rosana Pereira; Brondani, CláudioFusarium wilt, caused by the soil fungus Fusarium oxysporum f. sp. phaseoli, is a disease of great importance in the bean crop (Phaseolus vulgaris L.). Chemical control is not efficient and the use of resistant cultivars, within an integrated management strategy, is the most efficient way to control this disease. However, there is little information about the genetic control of the Fusarium wilt reaction and few carioca bean cultivars with satisfactory resistance level. Thus, three studies were carried out, with the objectives of: identifying parents and segregating populations of carioca beans with resistance to Fusarium wilt and other important characters; estimate genetic and phenotypic parameters and select carioca bean lines with resistance to fusarium wilt, high yield and mass of 100 grains and good visual appearance of grains; to study genetic control and identify molecular markers linked to QTLs that control the reaction to Fusarium wilt in the carioca bean cultivar BRS Notável. All evaluations were carried out in Santo Antônio de Goiás-GO, in the winter crop (sowing in May), in an area with high pathogen infestation, with central pivot irrigation. In the first study, 21 populations obtained from crosses in a complete diallel scheme between seven parents with grain carioca and some degree of resistance to Fusarium wilt were evaluated, together with two controls. Populations were evaluated in the F3, F4 and F5 generations, in 2012, 2013 and 2014, in a randomized block design, with three replications. The reaction to Fusarium wilt was evaluated using a grading scale, in addition to yield and mass of 100 grains. There were significant differences between populations for all traits evaluated. Additive and non-additive effects were important for Fusarium wilt reaction, yield and 100 grain mass. Cultivar BRS Notável was the most promising parent to participate in new crosses, presenting estimates of general combining ability (gi) different from zero, favoring the increase in resistance to fusarium wilt (-0.51), yield (87.90) and mass of 100 grains (0.23). The populations BRS Notável / CNFC 15872, BRS Ametista / BRS Notável, BRS Notável / BRSMG Talismã and BRS Ametista / BRSMG Talismã were the most promising for obtaining lines. In the second study, 114 lines obtained from BRS Notável / BRS Ametista and BRS Notável / CNFC 15872 populations, selected for their high resistance to Fusarium wilt, yield and mass of 100 grains, were evaluated. The 114 lines and seven controls were evaluated in experiments in triple lattice design 11 x 11, in the years 2015 and 2016, for reaction to Fusarium wilt and visual grain analysis, both using a grading scale, in addition to yield and 100 grain mass. Analysis of variance and genetic and phenotypic parameters were estimated. Genetic variability was detected among lines for the four characters. The high estimates of heritability, above 63%, and of expected gain with direct selection indicated the possibility of obtaining genetic gains for each character alone (23.0% for fusarium wilt reaction, 12.3% for yield, 9.6% for mass of 100 grains and 10.5% for visual appearance of grains). The simultaneous selection of 24 lines (21%) for the four characters showed gains of 12.9% for reaction to Fusarium wilt, 5.6% for yield, 3.4% for mass of 100 grains and 6.1% for visual aspect of grain. Three lines stood out (CNFC 19126, CNFC 19205 and CNFC 19131), being superior to BRS Notável for all characters. In the third study, the genotyping of SNPs and SilicoDArT markers was carried out using the DArTseq technology in the F2 generation of a mapping population obtained from the cross between the cultivars of Mesoamerican origin BRS Notável (resistant), of carioca grain, and BRS Supremo (susceptible), with black grains. Seventy-three progenies were evaluated for reaction to Fusarium wilt, by means of a grading scale, in the years/generations 2018/F2:4 and 2019/F2:5, together with the parents and five controls, in a block design at the random, with three repetitions. Analysis of variance and genetic and phenotypic parameters were estimated. A genetic map with 463 markers was obtained, with a total length of 1598.6 cM and average distance between markers of 3.5 cM. The analysis of phenotypic data showed variability between progenies and possibility of success with selection, with high estimates of genetic variance, heritability (90.6%) and expected gain with selection (28.5%), based on the joint analysis. Three QTLs were identified explaining between 25.1 and 50.4% of the phenotypic variation, all on chromosome 7, one in each of the evaluated years/generations (FOP7.2NS/ 2018 and FOP7.3NS/ 2019) and another based on in the joint analysis (FOP7.1NS). These three QTLs intersected between their intervals, with high stability in different years/generations. Therefore, the inheritance of the reaction in cultivar BRS Notável is quantitative, with the presence of some genes with greater effect. The additive effect for QTL FOP7.1NS was -1.24, indicating increased resistance in the presence of the BRS Notável parent allele. The SNP marker “SNP_Chr07_28294499_M12143”, present in all QTLs, explained 44.8% of the variation based on the pooled analysis. This marker is indicated to obtain probes for further validation.Item Modelos de predição genômica multi-ambiental em milho tropical: produtividade de grãos e staygreen(Universidade Federal de Goiás, 2023-04-28) Crispim Filho, Ailton José; Coelho, Alexandre Siqueira Guedes; http://lattes.cnpq.br/0840926305216925; Resende, Marcela Pedroso Mendes; http://lattes.cnpq.br/2080097211870591; Resende, Marcela Pedroso Mendes; Môro, Gustavo Vitti; Souza Júnior, Cláudio Lopes de; Costa Neto, Germano Martins Ferreira; Toledo, Fernando Henrique Ribeiro BarrozoStaygreen and grain yield are agronomic traits of interest to be evaluated in modern maize breeding programs. A modern approach to improving these traits can be genomic selection, whose efficiency depends, among other factors, on the proper choice of the prediction model to be used, the effects that will be accounted for in this model and the resources and time required for the prediction process of the phenotypes. In this work, three parametric models and a non-parametric model were used in the multi-environment genomic prediction of single maize hybrids for staygreen and grain yield, considering additive effects, exclusively, and together with dominance effects. The phenotypic data refer to the evaluation of 152 single maize hybrids, from the crossing of 42 inbred lines, evaluated in 13 environments for grain yield and 8 environments for staygreen. The lines were genotyped with 13,826 SNPs (Single Nucleotide Polymorphism) markers using the GBS (Genotyping by Sequencing) method, and their genotypic combinations were used to generate the genotypes of the hybrids. Adjusted means for each genotype at each location were used to train the genomic prediction models. The predictive ability was measured using Pearson's mean correlation, obtained using the ten-fold system. The models' predictive abilities ranged from 0.23 to 0.83 for grain yield and 0.44 to 0.72 for staygreen. The inclusion of dominance effects in all parametric models increased the predictive abilities for both traits, and for grain yield the average increase was 25%. This confirms that the inclusion of non-additive effects in the prediction model allows better exploration of heterosis and greater precision in genomic selection. The models did not differ between attributes linked to predictive ability. Due to the lower computational demand of GBLUP, it is the most suitable to predict the phenotypic performance of these characters in this data set. Prediction with the additive-dominant GBLUP model indicates the possibility of selecting better combinations of inbred lines than those already performed, which potentially increase grain and staygreen productivity by selecting the best 15 hybrids per prediction for each character separately.Item Variabilidade genética de Dipteryx alata Vogel (Leguminosae): novos marcadores microssatélites, fluxo de pólen in situ e ex situ e genética da paisagem em escala local(Universidade Federal de Goiás, 2018-02-23) Guimarães, Rejane Araújo; Telles, Mariana Pires de Campos; http://lattes.cnpq.br/4648436798023532; Soares, Thannya Nascimento; http://lattes.cnpq.br/5590256762396056; Soares, Thannya Nascimento; Mansano, Vidal de Freitas; Borba, Tereza Cristina de Oliveira; Chaves, Lázaro José; Diniz Filho, José Alexandre FelizolaDipteryx alata is a Neotropical tree widely distributed throughout the Brazilian Cerrado. Popularly known as baru, it is one of the most promising Cerrado species for domestication and cultivation due to its wide potential use. The general goal of this work was to assess reproductive system and pollen-mediated gene flow patterns of in situ and ex situ conditions and to evaluate how landscape may influence intrapopulation genetic structure. New microsatellite markers were developed using high performance sequencing technologies, then the ten most polymorphic were selected and used in this work. The evaluation of the reproductive system and pollen dispersal was carried out in a germplasm collection (ex situ) located at the Universidade Federal de Goiás and in one natural population (in situ) in Orizona-GO, central Brazil. The natural population was also used to evaluate landscape influence on intrapopulation genetic structure in adult and juvenile trees. In situ and ex situ conditions showed a high cross fertilization rate (tm = 0.815 and tm = 0.934), respectively, confirming that the D. alata presents a mixed-mating system, predominantly allogamous. The number of seeds sharing the same pollen donors was high, indicating a low number of pollen donors per tree under both conditions. This may be related to the proximity between groups of trees, considering that there is less cross-pollination between groups of more distant trees. Another factor that may have influenced cross-pollination over long distances is the heterogeneous landscape due to habitat fragmentation. The results indicate the presence of a positive and significant spatial genetic structure (SGS) in both stages of life (adults and juveniles) with the highest Sp value in juveniles. Bayesian cluster analysis showed the formation of two groups in both adults and juveniles. Juvenile individuals presented greater interference from landscape compositions. Thus, the inclusion of the landscape features brought an explanation gain to the pattern of intrapopulation genetic structure in the D. alata species. These results are important to support more efficient conservation strategies for this species.Item Melhoramento genético do feijão-comum assistido por marcadores moleculares: identificação, caracterização, mapeamento e piramidação de alelos de resistência a doenças(Universidade Federal de Goiás, 2022-10-11) Messias, Lucas Matias Gomes; Resende, Marcela Pedroso Mendes; http://lattes.cnpq.br/2080097211870591; Souza, Thiago Lívio Pessoa Oliveira de; http://lattes.cnpq.br/9650183308779143; Souza, Thiago Lívio Pessoa Oliveira de; Oliveira, Bruna Mendes de; Melo, Leonardo Cunha; Pereira, Helton Santos; Vianello, Rosana PereiraThe common bean's yield and commercial grain quality are constantly challenged by more than 45 diseases and post-harvest biochemical changes like grain darkening. The productivity, nutritional quality, and minor post-harvest changes (slow darkening) are of great importance for the acceptance of the product in the market. The aims of this study were: 1) mapping the genomic region associated with anthracnose resistance from a population (BRSMG Realce x BRS FC104) with 161 F2 plants that were phenotyped for reaction to race 475 of the fungi Colletotrichum lindemuthianum and genotyped with SNPs markers using the DArTseq technology; 2) Validation of SNPs markers previously identified as linked to anthracnose and angular leaf spot resistance alleles using a set with 376 genotypes, including elite lines, cultivars, and common bean landraces; 3) Establish an allele pyramiding program by marker-assisted selection for anthracnose, angular leaf spot, and fusarium wilt in common bean of carioca type with slow darkening of grains. The results shown that anthracnose resistance in BRSMG Realce is controlled by a single locus (R2=54,6%) with complete dominance, provisionally designated as CoPv04R, and mapped in a genomic region with 704,867 bp comprising 44 putative genes related to the pathogen-host interaction. The SNPs markers snp12782 (1,182,123 bp), snp3308 (505,696 bp) and snp1327 (477,285 bp) were indicated to monitor the CoPv04R allele introgression with a selection efficiency of 99,0%. Based on these results and the usefulness of CoPv04R for breeding purposes, it is proposed that the locus be officially nominated in accordance with the rules established by the Bean Improvement Cooperative Genetics Committee. Regarding the validation of markers previously identified as associated with bean diseases, of the nine evaluated SNPs, seven showed the potential to be incorporated into the assisted selection routine along with Embrapa's common bean breeding programs (snpPV0025-Phg-2; snpPV0027-Phg-5; snpPV0046-Co-u; snpPV0068-Co-42; snpPV0070-Co-42; snpP8282v3-817-Co-42; snpPV0079-Phg-5). The SNP markers snpPV0025 and snpPV0079 are indicated to monitor the Phg-2 and Phg-5 allele introgression, respectively. The genotyped system based on TaqMan™ assay in qPCR for the SNP markers snpPV0070, snpP8282v3-817, and snpPV0025 were specific for target-alleles Co-42, Co-42, and Phg-2, respectively. The markers snpPV0070, and snpP8282v3-817 show a selection efficiency of 99,0%. The target-allele pyramiding assisted by SNP markers snpP8282v3-817, and ANAAJK6 allowed the early selection of nine progenies combining the alleles Co-42 and FOP2.3403H in homozygosis, respectively. The selection with the marker PvbHLHp12804 (sdsd) was 100% coincident with the grains' slow-darkening phenotype after 140 storage days. Our results showed that the identification and validation of molecular markers identified as associated with target alleles for traits of agronomic importance in the common bean is essential to guide the choice of superior genotypes and improve selection efficiency, attributing speed to the genetic improvement program.Item Variabilidade genética quantitativa e estrutura populacional de Dipteryx alata Vog. do cerrado(Universidade Federal de Goiás, 2017-11-30) Mota, Elias Emanuel Silva; Soares, Thannya Nascimento; http://lattes.cnpq.br/5590256762396056; Chaves, Lázaro José; http://lattes.cnpq.br/9990967290797379; Chaves, Lázaro José; Novaes, Evandro; Soares, Thannya Nascimento; Nabout, João Carlos; Rodrigues, FabrícioThe objective of this study was to estimate genetic parameters for quantitative traits of Dipteryx alata; to infer about the influence of climatic, edaphic and geographic factors on the phenotypic divergence among subpopulations and to compare the population genetic structure of the species, based on quantitative and molecular data, to infer about the adaptive processes in the subpopulations. The germplasm collection consists of 600 plants in the field, in a randomized complete block design, with four replications and one plant per plot. Seedlings from the sowing in mid-October 2011 were transplanted to the field in March 2012. A phenotypic evaluation was also carried out in the 25 natural subpopulations, in which leaf and inflorescence data were collected from adult plants in six states of Central Brazil, sampling six plants per subpopulation. A composite sample of soil was also obtained with subsamples collected in the projection of the canopy of the plants. The evaluations of the plants from the germplasm collection, for the purposes of this study, began in March 2013, being the individual accessions characterized morphologically and agronomically. The quantitative data of the collection and the natural subpopulations were submitted to descriptive analysis, analysis of variance and correlation between the silvicultural characters. From the variance components, the genetic parameters of interest were also estimated. The comparison of the population genetic structure via microsatellite marker (FST) and quantitative data (QST) was performed based on the probability distributions of the values of the two estimates, which were generated by parametric bootstrap using 10000 resamples. There was significant variation between subpopulations and progenies within subpopulations, for most of the traits evaluated. There is genetic variability both among and within subpopulation, and its structuring is inherent to the evaluated variable. The traits, initial height, final height, initial diameter, final diameter, stem height and crown diameter showed higher heritability values, having a greater potential for genetic gain by selection and breeding. The geographic distances matrix showed low magnitude correlation only with the distances matrix of the phenotypic data of leaves and with the data matrix of the nutritional contents of leaves. The results of the Mantel tests suggested that the environmental factors (climate and soil), as well as the geographical distribution of the subpopulations are associated, even in small magnitude, with the phenotypic divergence among the subpopulations. Eighteen of the twenty seven characters evaluated for the species suggest that drift is the main cause of differentiation among subpopulations.Item Estrutura genética, fluxo gênico e sistema reprodutivo de Anacardium occidentale L. do cerrado(Universidade Federal de Goiás, 2014-11-22) Oliveira, Leciane Karita de; Collevatti, Rosane Garcia; http://lattes.cnpq.br/9979596352166630; Telles, Mariana Pires de Campos; http://lattes.cnpq.br/4648436798023532; Telles, Mariana Pires de Campos; Rodrigues, Flavia Melo; Almeida, Luciane Madureira de; Chaves, Lazaro José; Soares, Thannya NascimentoBecause constant degradation of the Cerrado the persistence of long-term populations in their natural habitat may be compromised, as a result of loss of genetic variability. To gene-rate useful information for the implementation of conservation programs in situ and ex situ to Anacardium occidentale L. ecotype from Cerrado, species popularly known as cajuzinho-do-campo. This work had the objective: evaluate, based on microsatellite markers, the ma-ting system, spatial pattern of gene flow intra-population, genetic structure of spontaneous populations and the germplasm collection of the Federal University of Goiás and the landsca-pe influence the genetic structure of populations. For the difference in scale and type of analysis of populations, the work was separated into three chapters. In the first, titled "Ma-ting system, space genetic structure and contemporary gene flow in Anacardium occidentale L ", analyzes were made to answer questions related to the reproductive system and evoluti-onary processes that occur within a population of the species, individuals were sampled three stages of life. As a result it was observed that the species has mating system predomi-nantly outcrossing, the difference between multilocus and single locus outcrossing rate was positive and significant, indicating 16.4% of outcrossing between related individuals. Only 0.05% of the seeds were from selfing. Was assigned paternity to 45% of the seeds. In all ma-trices multiple paternity was observed with 6.7 effective number of pollen donors in each matrix. The pollen pool received by matriz had significant difference (фFT = 0.124). The pol-len dispersal occurred in greater proportion to short distances (41 m), but may reach distan-ces greater than 130 m. The effective area of pollination was 9410 m2 and the effective size of the neighborhood of 18 individuals. The seed dispersal probably occurs at distances grea-ter than the sampled area, which limited the powers of parents of juvenile individuals. Found high genetic diversity within the populationwith reduction the values of adults for seeds and increased inbreeding. The adults showed high genetic neighborhood (262 individuals), re-sulting from weak spatial genetic structure. In the second chapter, titled "Diversity and gene-tic structure of spontaneous populations and germplasm collection of Anacardium occiden-tale L. ecotype from Cerrado", populations were analyzed distributed in the Cerrado to as-sess the genetic variability and its structure between populations, was also analyzed the germplasm collection as the variability and its representation compared to the population. Was observed that the populations have high genetic variability and significant inbreeding. The geographical distribution of genetic diversity and allelic richness showed pattern central - peripheral. Genetic differentiation was weak and significant (RST = 0.095), with a pattern of isolation by distance. Populations with distance of up to 140 m are more similar than expec-ted by chance. There were no significant signs of genetic bottleneck. Individuals in the germ-plasm collection showed high genetic similarity, genetic differentiation was higher among offspring than between populations (θS = 184 and θP = 0.014, respectively). The variability of the collection is representative as the existing variability in spontaneous populations. Fi-nally, in the third chapter titled "Effects pattern of landscape on the variability and genetic structure of populations Anacardium occidentale L. ecotype from Cerrado", we analyzed the genetic structure at the landscape scale to Goiás state populations. Was observed that the main type of matrix around the populations are remaining natural vegetation and pasture, with great variation in the remaining percentage. There was a relationship between the change in allelic richness and remaining percentage of natural vegetation and predominant matrix around the populations in the lowest values were found when the predominant type of matrix is the pasture. Inbreeding was related to landscape when characterized for 2 km scale, where there was a higher inbreeding when the predominant type of matrix is the pas-ture. Due to anthropogenic changes in the landscape there was genetic discontinuity between eight pairs of populations. These results indicate that the fragmentation and change in land use are influencing the genetic variability and gene flow of populations.Item Melhoramento molecular do feijão-comum para resistência múltipla a viroses(Universidade Federal de Goiás, 2021-06-14) Silva, Rodrigo de Souza; Faria, Josias Correa de; http://lattes.cnpq.br/5616359873902028; Souza, Thiago Lívio Pessoa Oliveira de; http://lattes.cnpq.br/9650183308779143; Souza, Thiago Lívio Pessoa Oliveira de; Melo, Patrícia Guimarães Santos; Pinheiro, Patricia Valle; Vianello, Rosana Pereira; Oliveira, Bruna Mendes deAmong the diseases that affect the common bean (Phaseolus vulgaris L.), viruses deserve to be highlighted, since they are difficult to control and cause partial or total yield and grain quality losses. In Brazil, the main viruses of beans are: the common mosaic, caused by the Bean common mosaic virus (BCMV), the golden mosaic, whose causal agent is the Bean golden mosaic virus (BGMV) and the soybean stem necrosis disease, caused by Cowpea mild mottle virus (CPMMV). The symptoms of CPMMV emerged in common beans more recently in agronomic performance tests with the transgenic cultivar BRS FC 401 RMD, which presents effective resistance to BGMV (event Embrapa 5.1). The symptoms of CPMMV were confused or hidden by the more severe symptoms of BGMV, and both viruses occur simultaneously in the field and are transmitted by the whitefly (Bemisia tabaci), mainly in Central region Brazil. Thus, two studies were carried out with the objectives of: i) develop, evaluate and select transgenic common bean progenies, second generation, with carioca market class, good agronomic performance and with multiple resistance to viruses BCMV, BGMV and CPMMV, and ii) to investigate genetic inheritance of the CPMMV and to genetically map loci of resistance to CPMMV in the carioca seeded cultivar BRS Sublime. In the first study, elite progenies were developed from crosses using the carioca seeded cultivars BRS Estilo and BRS Sublime (both showing resistance to CPMMV and BCMV), with the transgenic line CNFCT 16206 (event Embrapa 5.1; effective resistance to BGMV) and resistant to BCMV. The recovery of elite progenies with a greater proportion of parents was performed through the analysis of backcrosses assisted by SSR and SNP markers. Thus, 39 elite progenies were evaluated in field condition, in the rainy (2016) and dry (2017) growing seasons, and in an insect-proof screenhouse. The evaluated traits were the seed yield and mass of 100 seeds, plant architecture and tolerance to lodging, seed appearance, and virus disease severity (VS). The elite progenies in addition to the controls were mechanical inoculated at eight days after planting with the strain (CPMMV: BR:GO:14 – GenBank MK202583), and were evaluated, at 35 days after inoculation, for severity of CPMMV. These progenies and the controls were also evaluated for the presence of the event Embrapa 5.1 (marker Ahas) and gene I (marker SCAR SW13). The result of the joint analysis showed variability between the progenies for all characters evaluated, especially SV, except for ARQ, considering the two environments, with P × E interaction (P ≤ 0.01). All elite progenies showed effective resistance to BCMV and BGMV, with the exception of progeny 336-3.1 (BGMV), whereas the conventional controls were susceptible to BGMV, and the transgenic control (cv. BRS FC401 RMD,) and the progenies were susceptible to CPMMV. Thus, the severity of CPMMV was assessed in progenies and transgenic controls. Twelve elite progenies showed mean scores ≤ 3.0 for VS. Of these, the progenies 184-12.1, 356-5.1, 398-3.1, 417-22.1 and 422-39.1 showed resistance to BCMV, BGMV and CPMMV. Therefore, the use of conventional breeding strategies and marker-assisted selection (SAM) enabled the development and selection of elite transgenic carioca-seeded cultivars, second-generation, with carioca-seeded market class, better plant architecture and allowing direct mechanical harvest, with multiple resistance to viruses and presenting potential to be evaluated in agronomic performance tests. In the second study, the genotyping of SNP and SilicoDArT markers was performed using the DArTseq technology, in a mapping population in the F2 generation obtained from the crossing between the cultivar BRS Sublime (resistant) e transgenic line CNFCT 16207 (susceptible). All F1 plants were tested with molecular marker linked to the event Embrapa 5.1 to confirm the hybrid nature. The plants were conducted in an insect-proof screenhouse. Thus, a total of 180 F2 individual plants and 180 F2:3 progenies (2160 seedlings; 12 seedlings/progeny), in addition to the parents, were mechanically inoculated with the same CPMMV isolate, at eight days after planting. At 35 days after inoculation, the plants were evaluated for severity of CPMMV. The phenotypic data were subjected to the chi-square test (ꭕ2) (P ≤ 0.05), considering the expected mendelian segregation ratio for each generation. The complete genetic map was obtained with 1.695 markers segregated according to the expected frequency and distributed in the 11 chromosomes of the bean, with total length of 2.864 cM and average distance between marks of 1.8 cM. The results indicated that a single dominant gene controls the inheritance of CPMMV resistance in the cultivar BRS Sublime. The QTL – single Gene (CPMMV. Pv08) was identified in the final region of chromosome 8, associated with resistance to CPMMV and explaining ~77% of the phenotypic variation. This is the first report to study genetic inheritance and genetically map locus to CPMMV resistance in BRS Sublime. The construction of the genetically map and the QTL analysis approach generates new perspectives for common bean breeding programs, with the potential for development and validation of molecular markers to be used through SAM for reaction to CPMMV in common bean genotypes.Item Melhoramento de feijão preto para resistência à murcha de fusário: identificação de marcadores moleculares e seleção de populações segregantes e linhagens(Universidade Federal de Goiás, 2020-12-10) Torres, Mário Henrique Rodrigues Mendes; Souza, Thiago Lívio Pessoa Oliveira de; http://lattes.cnpq.br/9650183308779143; Pereira, Helton Santos; http://lattes.cnpq.br/0729719587905292; Pereira, Helton Santos; Melo, Patrícia Guimarães Santos; Melo, Leonardo Cunha; Vianello, Rosana Pereira; Bruzi, Adriano TeodoroFusarium wilt, caused by the fungus (Fusarium oxysporum f. sp. phaseoli) is one of the most important diseases affecting bean culture (Phaseolus vulgaris L.). Among the methods of controlling this disease, genetic resistance is the most efficient. However, there is little information on the genetics of the fusarium wilt reaction (FOP). Three studies were carried out with the objectives of: i) selecting promising parents and the segregating populations for reaction to fusarium wilt, grain productivity and 100 grain mass; ii) estimate genetic parameters and select breeding lines resistant to fusarium wilt, with high productivity and greater mass of 100 grains; iii) check if there is a genetic relationship between these traits; iv) study genetic control and identify SNP molecular markers linked to QTLs for reaction to fusarium wilt in the bean cultivar BRS FP403. In the first study, 25 segregating populations obtained from crosses in the partial diallel scheme between two groups of the five parents were evaluated: group I with breeding lines resistant to fusarium wilt and group II with susceptible breeding lines, but with superior agronomic characteristics. The populations were evaluated in Santo Antônio de Goiás-GO in an area with natural infestation of the pathogen, in the winter / 2016 (generation F2), winter / 2017 (generation F3) and winter / 2018 (generation F4). The evaluated traits were the reaction to fusarium wilt, productivity and mass of 100 grains. Significant differences between populations were detected for all traits. The joint diallel analysis showed the existence of additive and non-additive effects, with a predominance of additive effects, for the three traits studied. The gi estimates revealed that the parents BRS Esplendor (-0.13) and CNFP 15207 (-0.76) are indicated to form populations with greater resistance to fusarium wilt. The CNFP breeding lines 15194 is indicated to form populations with greater resistance to fusarium wilt (-0.59) and greater mass of 100 grains (0.69). The cultivar BRS FP403 is indicated as the parents for increasing productivity (218) and mass of 100 grains (1.46). Among the susceptible parents, CNFP 11995 stood out for forming populations with high productivity and a mass of 100 grains, simultaneously. The populations BRS FP403 / CNFP 11995, CNFP 15194 / CNFP 11995 and CNFP 15194 / CNFP 11976 are the most promising for the extraction of superior breeding lines for the three traits simultaneously. In the second study, breeding lines from two populations were evaluated (BRS Esplendor / BRS Expedito and BRS Expedito / CNFP 15867), selected for their high resistance to fusarium wilt, high productivity and greater mass of 100 grains. Two experiments were conducted in the winter crops of 2015 and 2016, in Santo António de Goiás-GO, with 116 breeding lines from these two populations and five witnesses in a triple 11x11 latex experimental design, in an area infested with the pathogen. Reaction to fusarium wilt, yield and mass of 100 grains were evaluated. Analysis of variance and estimated genetic parameters were performed. Differences between lineages were identified in all environments for all traits. The estimates of heritability and expected gain with direct selection indicated the possibility of obtaining genetic gains for each traits in isolation. In the simultaneous selection of the breeding lines for the four traits, based on the joint analysis, the expected gains with the selection of the 31 best breeding lines were 22.1% for reaction to fusarium wilt, 6.6% for productivity and 7.7% for mass of 100 grains. Five breeding lines have high potential to be indicated as new cultivars, as they showed resistance to fusarium wilt, high productivity and a mass of 100 grains, being superior to the cultivars available on the market today. In the third study, genotyping of SNPs and SilicoDArT markers was performed using the DArTseq technology of a population mapping in the F2 generation obtained from the crossing between the cultivars BRS FP403 (resistant) x BRS Horizonte (susceptible). 165 progenies were obtained and evaluated in generations F2: 4 and F2:5, together with four controls. The experiments were installed in a 13x13 triple lattice design, in an area infested with the pathogen, in the winter/2016 (F2:4) and winter/2017 (F2:5) harvest in Santo Antônio de Goiás - GO. Evaluation of the reaction to fusarium wilt was performed. A genetic map was obtained comprising 702 markers with a total length of 3069 cM and an average distance between marks of 4.9 cM. The analysis of phenotypic data showed the presence of variability between the progenies and the possibility of success with the selection, with high estimates of genetic variance, heritability (90%) and expected gain with the selection (37%). Considering the two years and the joint analysis, 6 different QTLs were identified associated with the reaction to the fusarium wilt and there was an interaction between the QTLs and the years. Based on the joint analysis, four QTLs were identified on chromosomes 1, 2, 3 and 4, explaining from 5.8 to 40.5% of the variation, indicating that the inheritance of the reaction to fusarium wilt is complex. The QTL FOP2.3403H stood out for explaining the greater proportion of the phenotypic variation (40.5%) and being stable in the different years. The QTL FOP3.2403H explained 6.3% of the phenotypic variation and was also stable over the years. These markers have great potential for use in assisted selection.