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Item Toxoplasmose gondii: soroprevalência, isolamento e virulência de cepas obtidas de galinhas caipiras (Gallus domesticus) comercializadas em feiras livres do município de Goiânia(Universidade Federal de Goiás, 2007-02-27) Aleixo, Eduardo da Costa Alves; Castro, Ana Maria de; http://lattes.cnpq.br/9232309971000621; Soares, Joanna Darc Aparecida Herzog; http://lattes.cnpq.br/6933335902851476; Soares, Joanna Darc Aparecida Herzog; Gomes, Abraão Garcia; Bezerra, José Clecildo BarretoIn this study, 50 free-range chickens were obtained from free fairs of the city of Goiânia, its serum were tested for antibodies anti T. gondii with the modified agglutination test (MAT), its heart and brain had been processed and inoculated in groups of 6 mice. Samples of the organs of the birds and the surviving mice had been sectioned and stained with Hematoxylin-Eosin for the search of Toxoplasma-like cysts structures. The serum of the surviving mice was analyzed by the indirect immunofluorescence reaction (RIFI). The isolated ones had been submitted to the study of virulence for mice by inoculations with different concentrations of tachyzoites. 25 birds (50%) had revealed positive (MAT ≥ 1:5) getting a total of three isolated. Toxoplasma-like cysts structures had been found in histologic cuts of eight birds (16%) and organs of mice of two groups (2%). Of the 25 experiments with inoculeted of positives birds, in 11 (44%) it had the detention of IgG anti-Toxoplasma. In the samples of negatives birds, evidences of the presence of Toxoplasma had not been found. Of the three isolated, two had been lethal for mice from concentrations of a thousand tachyzoites., while another one was 100% fatal one from the concentration of ten a thousand tachyzoites.Item Avaliação clínica, laboratorial e histopatológica do efeito de drogas imunossupressoras na reativação da toxoplasmose crônica em modelo murino com a cepa ME 49 no camundongo BALB/c(Universidade Federal de Goiás, 2008-03-28) Alves, Fabiana Santiago Aleixo; Lino Junior, Ruy de Souza; http://lattes.cnpq.br/0372118837748010; Soares, Joanna Darc Aparecida Herzog; http://lattes.cnpq.br/6933335902851476; Garciazapata, Marco Tulio Antonio; http://lattes.cnpq.br/3672512339058369; Garciazapata, Marco Tulio Antonio; Lacerda, Elisângela de Paula Silveira; Carvalhaes, Mara SilvaThis work was evaluated for the potential reactivation of Toxoplasmosis in murine model similar to human immunosuppression being developed in BALB/c mice, which were 40 days infected with 20 cysts of ME 49 strain of Toxoplasma gondii and after 60 days was initiated treatment with immunosuppressant drugs, Azathioprine in dosage of 10mg/kg five times a week in days, Dexametasone in dosage 2.5mg/kg per day per mouse, three times per week on alternate days, Cortisone acetate in 50mg twice a week in subcutaneous injection of cortisone acetate alone, or associated with its proper controls. Treatment was continued for 28 days. The use of the Dexametasona or Azathioprine isolated or associated with, a factor not caused by reactivation of serological tests, clinical or histopathological but associated with cortisone acetate led to a clinical diagnostic framework of a voluntary recall because of injuries in epidermal 62.5% of the mice that lot compared to its proper controls, however mortality was not observed in any of the groups testedItem Carga epidemiológica da coqueluche e avaliação de impacto de vacinação de gestantes contra coqueluche implementada no Brasil em 2014(Universidade Federal de Goiás, 2022-08-10) Bagattini, Ângela Maria; Toscano, Cristiana Maria; http://lattes.cnpq.br/3772312631884265; Toscano, Cristiana Maria; Morais Neto, Otaliba Libânio de; Siqueira Júnior, João Bosco; Silva, Lara Lívia Santos da; Kuchenbecker, Ricardo de SouzaIntroduction: Pertussis is an acute infectious disease of respiratory transmission, with cyclical occurrence, it is endemic worldwide, represents an important global burden, particularly in children under one year of age who have more severe conditions and may progress to death. Between 2010 and 2014, a sudden and atypical increase in the number of cases of the disease was observed in several countries, including Brazil. There are several hypotheses about the factors associated with this resurgence, including disease cyclicality, replacement of whole-cell vaccines with acellular vaccines, falling vaccine coverage in children, and the effectiveness and duration of protection of vaccines in children, among others. Objectives: This study aims to characterize and estimate the epidemiological situation of pertussis in Brazil, evaluate the impact of vaccination of pregnant women with pertussis vaccine (dTpa) implemented in Brazil in 2014. Also, review the evidence about protection and duration of protection conferred by the whole cell vaccine against pertussis used for vaccination of children in the National Programs of Immunization. Methods: A study in three stages was carried out. The first stage described the epidemiological situation of whooping cough in morbidity and mortality in Brazil from 2000 to 2016. Next, the second stage with an interrupted time series ecological study was carried out with data adjusted by month and using the ARIMA model to assess the impact on cases and hospitalizations of children under five years of age with the introduction of the dTpa vaccine for pregnant women in 2014. The two stages used three independent health information systems, the Information System for Notifiable Diseases (SINAN), the Hospital Information System (SIH), the Mortality Information System (SIM) and the National Immunization Program Information System (SI-PNI), data were aggregated and evaluated by age group and federation unit. The third stage of the study was carried out through a systematic review to assess the effectiveness and duration of protection provided by the whole cell pertussis vaccine (DTPw) in children considering the vaccines currently available on the international market. Results: Pertussis showed cyclical patterns of disease burden over time between 2000 and 2016 in Brazil, in different regions with heterogeneous conditions, with a well-defined outbreak that started in 2011 and peaked in 2014, reaching mainly and more severely. children under six months, with 20,103 (54%), 19,919 hospitalizations (79%) and 565 deaths (93%). The incorporation of dTpa vaccination in pregnant women was associated with a significant reduction in pertussis cases and hospitalizations in children under six months of age when coverage is above 45%. After the introduction of dTpa, it is estimated that 2,124 cases and 1,439 hospitalizations for pertussis were avoided in children under six months of age in states with coverage above 45% between 2015 and 2016. Additionally, 12 studies with DTwP conducted between 2007 and 2020 were included for review, which have varied methodological quality and lack evidence on immunogenicity and duration of immunity indicating a short duration, less than five years. Conclusions: The analysis of different health information systems used showed consistent results throughout the period analyzed, reflecting the cyclicity of the disease and its resurgence from 2011. The incorporation of dTpa vaccination in pregnant women resulted in an impact on the reduction of hospitalizations and deaths of children when vaccination coverage above 45% is achieved. The wP vaccines currently in use have scant evidence of duration of immunity, even though they are used in over 100 countries, in most low- and middle-income countries. Relevance and Impact: The results of this work reinforce the importance of achieving and maintaining dTpa vaccination coverage in pregnant women above 45% in order to obtain a significant impact of vaccination of pregnant women in reducing hospitalizations for pertussis in children. Although wP is one of the most used vaccines globally in children in immunization programs in low- and middle-income countries, there has been an important change in the producers of these vaccines in recent decades, with large pharmaceutical companies having left the market and being replaced by producers in countries emerging technologies that today account for the totality of wP vaccines produced and used in the world. Evidence suggests that the duration of protection afforded by these vaccines in children is short. However, better quality evidence on the effectiveness and duration of immunity conferred by these vaccines is needed to support the definition of more appropriate vaccination strategies.Item Avaliação da produção de BMP2 no intestino de camundongos infectados com Trypanosoma cruzi(Universidade Federal de Goiás, 2020-03-12) Carmo Neto, José Rodrigues do; Silva, Juliana Reis Machado e; http://lattes.cnpq.br/5289363102869037; Silva, Juliana Reis Machado e; Oliveira, Milton Adriano Pelli; Campos, Helioswilton Sales de; Freitas, Aline de AraújoOne of the main late complications of Chagas' disease (CD) is the megacolon, affecting approximately 10% of symptomatic patients. However, studies are needed to understand mechanisms involved in the progression of this condition in the chronic phase of CD. Myenteric plexus neurons are known to be essential for the control of intestinal motility. Through infection by Trypanosoma cruzi (T. cruzi), the inflammatory profile that sets in is involved in neural destruction. One of the proteins related to the maintenance of nerve cells in the myenteric plexus is the type 2 morphogenetic protein (BMP2), produced mainly by muscle macrophages. The homeostasis of the BMP2/macrophage ratio is directly involved with intestinal motility and the maintenance of organ function. Thus, the aim of this study was to correlate the production of intestinal BMP2 with the production of cytokines and histopathological changes in C57Bl / 6 mice. infected by the T. cruzi Y strain in the periods of 30 and 90 days of infection. The mice were infected with 1000 blood trypomastigote forms. After the infection period, the mice were euthanized and the spleen and intestine were collected. The intestine was divided in two, one fragment was used for histological analysis and the other for quantification of TNF-α, IFN-γ, IL-10 and BMP2, as well as the spleen. Infection with strain Y induced an increase in the production of TNF-α, IFN-γ and BMP2 in the intestine after 30 days of infection, as well as an increase in the inflammatory infiltrate and a decrease in the number of neurons in the myenteric plexus. Collagen deposition increased gradually throughout the infection, demonstrated at 90 days of infection. It was observed that the increase in BMP2 after 30 days of infection has a positive correlation with the increase of IFN-γ and TNF-α in the intestine. However, BMP2 and IFN-γ showed a negative correlation with the number of neurons in the myenteric plexus in the same period in the organ. As a first report of the alteration of BMP2 production after infection by T. cruzi, it is suggested that this imbalance may represent a new pathway in maintaining the intestinal pro-inflammatory profile, as well as being related to the neuronal damage that the infectious process establishes.Item Reação imunoenzimática (ELISA) para detecção de imunoglobulina M, imunoglobulina G e imunoglobulina A contra a proteína rHsp-X (Rv 2031c) de mycobacterium tuberculosis em pacientes com tuberculose pleural(Universidade Federal de Goiás, 2009-12-18) Limong, Loanda Carvalho Sant’ Ana; Kipnis, Ana Paula Junqueira; http://lattes.cnpq.br/1252262903952987; Kipnis, Ana Paula JunqueiraPleural tuberculosis (TBP) has often spontaneous resolution, even without treatment. However, the lack of proper diagnosis and treatment can lead to the occurrence of pulmonary and/or extra-pulmonary tuberculosis in 65% of the cases, in the five years subsequent. The objective of this study was to evaluate the effectiveness of the recombinant Hsp-X antigen (rHsp-X) in an enzyme immunoassay for determining the presence of TBP in patients with pleural effusion. For this study we used 132 samples of serum and pleural fluid (PF) from patients with pleural effusion. Of these, 97 samples were from TBP patients and 35 patients with diseases other than tuberculosis (NTBP) (28 patients with metastatic cancer, 1 patient with congestive heart failure, 2 patients whit liver failure, 1 patient with systemic lupus erythematosus and 3 patients with parapneumonic effusion). The IgM levels of PF were higher in patients with PTB than NTBP, the sensitivity and specificity of the ELISA for the PF was 42% and 83%, respectively. Testing for IgG from the serum and the PF against the recombinant antigen Hsp-X were not effective in discriminating TB patients from other diseases, the sensitivity was found to be 13% and 16% using serum and LP, respectively, with the same specificity of 83%. TBP patients presented higher levels of IgA specific for the rHSP-X than NTBP, the IgA ELISA using PF presented better sensitivity (65%) than using serum (39%), while the IgA ELISA for both types of samples presented the same specificity (83%). In conclusion, the recognition of Mycobacterium tuberculosis rHsp-X by IgA antibodies from the PF of patients with TBP reveals its possible role in the development of a diagnostic test for additional cases of TBP. Further studies must be conducted to confirm this hypothesis.Item Análise das interações proteína-proteína da chaperona de cobre ATX1 em Paracoccidioides brasiliensis(Universidade Federal de Goiás, 2022-08-12) Lugo, Danize Eukales Menezes; Silva, Kleber Santiago Freitas e; http://lattes.cnpq.br/3813868830071259; Soares, Celia Maria de Almeida; http://lattes.cnpq.br/8539946335852637; Soares, Celia Maria de Almeida; Pereira, Maristela; Bailão, Alexandre MeloParacoccidioidomycosis is the most prevalent systemic mycosis in the Americas, mainly in Brazil, Colombia, Venezuela and Argentina. The disease is caused by fungi of the genus Paracoccidiodes that exhibit thermodimorphism. The fungus is present in the soil in the mycelial form at 28°C and in the yeast form in the host at 37°C. The ability to differentiate is considered a virulence factor of this pathogen. Copper (Cu) is an essential component of enzymes that carry out electron transfer reactions. Homeostasis of this metal was first described in Saccharomyces cerevisiae and homologous genes were identified in several organisms. Among these, the ATX1 gene, related to a copper metallochaperone, stands out, which transports Cu1+ from Ctr1 (transmembrane transporter) to Ccc2 (P-type ATPase) in a trans-Golgi vesicle for eventual insertion into Fet3. The latter is a highaffinity Cu-dependent iron absorption protein. Atx1 was identified in S. cerevisiae as a small 8 kDa Cu chaperone, being classified as an antioxidant molecule. Little is known about Cu homeostasis metabolism and the specific function of the ATX1 gene in Paracoccidioides brasiliensis. Thus, the identification of Atx1 interaction networks in P. brasiliensis can elucidate details of Cu metabolism in this organism. The main objective of this research was to characterize, through molecular anchoring, how ATX1 interacts with P. brasiliensis proteins, providing an understanding of the biology of the fungus and aiming to identify possible therapeutic targets as a future perspective. A group of proteins that interact with Atx1 was identified and validated through pull-down assays. These proteins are part of the maintenance of homeostasis, interacting with other proteins of copper metabolism, electron transport and detoxification proteins. These interactions indicate the importance of Atx1 for maintaining copper homeostasis in the fungus, being a potential target for alternative drugs, which may collaborate to expand therapeutic options.Item Características clínicas e laboratoriais de uma doença neurológica rara e hereditária em uma família do Brasil central(Universidade Federal de Goiás, 2016-06-09) Medeiros, Rodrigo Parente; Caixeta, Leonardo Ferreira; http://lattes.cnpq.br/9536747113677509; Caixeta, Leonardo Ferreira; Teixeira, Kim Ir Sen Santos; Mendonça, Helena Rezende SilvaA rare disease is defined by the World Health Organization as diseases that affect up to 65 people / 100,000. To evaluate these diseases it is required to be performed a lot of tests and it is often not possible to reach a definitive diagnosis. The aim is to describe the clinical and neuroimaging aspects in a countryside Goiás’ family that carries a rare neurodegenerative disease. It’s a descriptive study about five brothers. It’s conducted physical examinations, laboratory, MRI of the cervical column, dorsal and lumbar besides the skull. Made neurophysiological diagnosis, Through genetic sequencing to make exome. We were subjected to neuropsychological assessment, the MEEM and FIM. The five brothers have in common the tetraparesia of crural predominance , muscular atrophy of the lower limbs , severe mental retardation early onset , cerebellar atrophy , paravertebral atrophy and motor axonal neuropathy. Made sequencing the exome for research related genes hereditary spastic paraparesis and not found any gene related to this disease. A probable diagnosis would be the spinocerebellar ataxia type 13, due to the reported clinical picture. But it is necessary a genetic study to proof it. Continuous genetic evaluation of these patients is important because of the description of new genes.Item Saúde mental e trabalho: prevalência de morbidade psiquiátrica e fatores associados, entre servidores municipais em licença médica, Goiânia-Goiás, 2010(Universidade Federal de Goiás, 2013-09-13) Mendonça, Mauro Elias; Turchi, Marília Dalva; http://lattes.cnpq.br/3769826743537934; Turchi, Marília Dalva; Corrêa Filho, Heleno Rodrigues; Siqueira Júnior, João BoscoIntroduction: Mental disorders (MD) have high morbidity and high load in the years lived with disability, reducing quality of life and producing large socioeconomic impact. Objective: To estimate the prevalence and potential factors associated with psychiatric morbidity of municipal employees on sick leave in Goiânia, Brazil, in 2010. Methods: Cross-sectional census study linking Human Resources System with records of licenses issued by the Medical Board municipality. Socio-demographic and occupational variables and duration of the associated removal by MD licenses were analyzed. The level of significance was set at <5%. Results: Among the 20,535 servers, 5,028 (24.5%) had at least one license, with 801 of these for psychiatric morbidity. In total 9,101 licenses, of which 1,569 were granted for MD. The MD represented the greatest burden of the total period of absence (22.1%) with 75% of the servers on leave for > 15 days. The prevalence of MD was 39.0 licensed servers/1.000 active servers, with 76.4 licenses/1.000 active servers. The prevalence of MD was higher among women and servers with primary or higher education level. There were differences in the leave of absence profile caused by DM between men and women regarding age, ethnicity, marital status and salary. In relation to work, there was a higher prevalence of MD on servers with double bond, length of service <3 years (probation) and with 11-20 years of work. The highest prevalence of MD among women, was found in positions of education and health, and, among men, in surveillance jobs. Mood disorders (25.4/1,000) and neurotic (16.9/ 1,000) were clusters of higher prevalence, with high co-morbidity between both. The diagnostic category of depression was more prevalent. Discussion and Conclusions: In the present study, the prevalence of MD was more than six times higher than the one found on workers of the general social security system in the country. The results confirm the relevance of MD in health of servers, and bring subsidies to boost promotion measures, surveillance and occupational health care. The need to deepen the study of psychosocial factors associated with high prevalence of psychiatric morbidity in the public sector and differences in gender and occupation was pointed out in this study.Item Tendência da mortalidade por pneumonia em idosos no Brasil e o contexto da vacinação pneumocócica(Universidade Federal de Goiás, 2019-06-12) Miranda, Denismar Borges de; Andrade, Ana Lúcia Sampaio Sgambatti de; http://lattes.cnpq.br/7770363683068899; Morais Neto, Otaliba Libânio de; http://lattes.cnpq.br/4030124246791320; Moraes Neto, Otaliba Libânio de; Turchi, Marília Dalva; Souza, Maria de Fátima Marinho de; Bierrenbach, Ana Luiza; Sartori, Ana LúciaCommunity-acquired pneumonia is one of the most common infectious diseases and is considered a major cause of morbidity and mortality in the elderly population worldwide. Studies show the direct and indirect impact of 10-valent pneumococcal vaccine on hospitalizations for pneumonia in several countries. There is scarce knowledge regarding this impact on mortality in the elderly in the world and, to date, no evidence in the Brazilian population. This study aimed to propose models for correcting mortality rates due to pneumonia in the elderly in Brazil and to evaluate the indirect impact of PCV-10, introduced in the childhood immunization schedule, at these rates. This is a time-series study of mortality rates from pneumonia in the elderly from 2005 to 2016. For the time-series analysis, the models for predicting pneumonia rates, if the vaccine had not been implemented, were Seasonal Autoregressive Integrated Moving Average (SARIMA) in the presence of seasonality, Auto Regressive Integrated Moving Averages (ARIMA) when there was only trend, and Autoregressive Moving Average (ARMA) in the absence of trend and seasonality. Percentage difference between observed and predicted rates were calculated, considering statistical significance of 5%. There was an increasing trend of mortality due to pneumonia in the elderly in Brazil. Interrupted time series analysis showed that the estimated pneumonia mortality rates from the study were significantly lower than those predicted by the analysis models. There was probably an indirect impact of PCV-10 on the elderly. In the Southeast region there was a statistically significant difference between the rates observed and the predicted in the three age groups of the elderly.Item Identificação por imunoproteômica dos exoantígenos do complexo paracoccidioides, com potencial aplicação no diagnóstico e terapia da paracoccidioidomicose(Universidade Federal de Goiás, 2019-08-26) Moreira, André Luís Elias; Bailão, Alexandre Melo; http://lattes.cnpq.br/5415221996976886; Bailão, Alexandre Melo; Borges, Clayton Luiz; Silva Neto, Benedito Rodrigues da; Rocha, Thiago Lopes; Oliveira, Milton Adriano Pelli deIdentification by immunoproteomic of exoantigens of the Paracoccidioides complex, with potential application in diagnosis and therapy of Paracoccidioidomycosis Fungi of Paracoccidioides complex are the etiological agents of Paracoccidioidomycosis (PCM), a systemic mycosis restricted to Latin American countries. Currently, the Paracoccidioides genus is represented by P. lutzii, P. americana, P. brasiliensis, P. restrepiensis and P. venezuelensis. In some cases, oral and skin mucosal lesions caused by other pathogens may coincide with lesions caused by Paracoccidioides spp.. Moreover, even with the advances in immunological techniques used for the diagnosis of fungal diseases, false-positive results rates for PCM are present. Thus, we investigated which antigens are secreted by 4 species of the Paracoccidioides complex in order to identify and characterize new molecules, thus increasing the spectrum of antigens to be used for future diagnostic tests of PCM. Through of nanoUPLC-MSE, 79 exoantigens were identified in 4 Paracoccidioides species. In silico analysis revealed 2 exoantigens exclusive to P. lutzii species, as well as the identification of 44 unique B-cell epitopes of the Paracoccidioides complex. Thirteen exclusive epitopes distributed among Paracoccidioides species also predicted, being this excellent molecules to be employed in the future for epidemiological studies. These results demonstrate a range of epitopes exclusive to the Paracoccidioides complex as well as the identification of molecules unique to each fungal species. In addition, these analyzes allowed the identification of new candidate biomarkers to PCM diagnosis, as well as the identification of molecules to be used as future epidemiological biomarkers.Item Rastreamento sorológico da infecção pelo vírus da hepatite C em cortadores de cana-de-açúcar nos estados de Goiás e Paraíba(Universidade Federal de Goiás, 2019-02-22) Oliveira, Brunna Rodrigues de; Carneiro, Megmar Aparecida dos Santos; http://lattes.cnpq.br/8398563469665169; Carneiro, Megmar Aparecida dos Santos; Martins, Regina Maria Bringel; Teles, Sheila AraujoIntroduction: Hepatitis C virus (HCV) infection is a major problem for a global public health, with about 71 million people chronically infected worldwide, being a major cause of cirrhosis and hepatocarcinoma. The World Health Organization (WHO) set the goal of eliminating viral hepatitis as a public health problem by 2030. To do so, it is necessary to track infections in all populations. Sugarcane cutters are a population of seasonal workers, with difficulty to access to health services, which lack information on hepatitis C. Objective: This study aimed to perform the serological screening of hepatitis C virus infection in sugarcane cutters in Goiás and Paraíba. Methods: The population consisted of 937 sugar cane cutters, 636 in Goiás and 301 in Santa Rita-PB. All cutters were invited to participate in the study, and those who signed the consent form were interviewed using a standardized questionnaire. Then, blood samples were collected and screened for anti-HCV by rapid tests and ELISA. The data were analyzed in the statistical program SPSS version 17.0 for Windows. Results: The population was composed of males (100%), with a mean age of 35.4 years. Regarding schooling, 47.4% reported having up to four years of study and the monthly family income of 78.8% of the participants was less than 2,000.00 reais. After screening for anti-HCV by rapid test and ELISA, it was verified that no individual was exposed to HCV. The risk characteristics for potential viral spread reported by cane cutters were tattooing/piercing, sharing of personal use material, and use of non-injectable drugs. Rapid tests are highly sensitive to anti-HCV, so they are appropriate and feasible for hard-to-reach populations such as sugarcane cutters and other populations. Conclusion: The present study showed that there was no exposure to HCV in the sugarcane cutters of the regions of Goiás and Santa Rita-PB. To achieve WHO's goal of eliminating viral hepatitis as a public health problem, it is important that more research on HCV prevalence is conducted, especially in groups that have difficulty accessing the health system.Item Epidemiologia molecular de isolados de toxoplasma Gondii na região metropolitana de Goiânia, Goiás, Brasil(Universidade Federal de Goiás, 2018-01-12) Rezende, Hanstter Hallison Alves; Vinaud, Marina Clare; http://lattes.cnpq.br/1921551651088660; Castro, Ana Maria de; http://lattes.cnpq.br/9232309971000621; Castro, Ana Maria de; Fernandes, Everton Kort Kamp; Rocha, Thiago Lopes; Cardoso, Cléver Gomes; Soave, Danilo FigueiredoThe protozoan Toxoplasma gondii is the etiological agent of toxoplasmosis and its definitive hosts are domestic cats (Felis catus) and other wild felids. Within the cities, stray cats are responsible for the dissemination of the parasite in the environment as they release oocysts which are the infective forms for humans and other animals. Backyard chickens (Gallus gallus) are in constant contact with the environment and feed directly from the soil. Therefore they are important indicators of environmental conditions. The knowledge regarding prevalence, biological and genetic characteristics of T. gondii are of utmost importance for the comprehension of the complex host-parasite relationship. Thus the aim of this study was to evaluate the concordance of laboratory techniques used to detect the parasite; to determine the prevalence, biology and molecular epidemiology of T. gondii isolated from stray cats and backyard chickens from the metropolitan region of Goiania, in order to better comprehend the infection. This is the first performed study to determine the genetic and biologic characterization of T. gondii in the state of Goias. This study used 24 stray cats captured by the Center for the Zoonosis Control in Goiania and 50 backyard chickens from the Metropolitan Region of Goiania, Goias. The serologic triage was performed by IHA and showed positivity of 87.4% (21/24) cats and 96% (48/50) backyard chickens for T. gondii. The bioassay was performed using the brain and hearts of the cats and chickens obtained after euthanasia of the animals. After the peptic digestion the homogenate tissues was intraperitoneally inoculated in groups of three Swiss mice each which were daily observed in order to detect signs of acute toxoplasmosis. The asymptomatic mice were euthanized after 60 days followed by serologic analysis through indirect immunofluorescence. Fragments of brain from these animals were observed under optic microscopy in order to visualize tissue cysts. Part of the homogenate was submitted to DNA extraction and polymerase chain reaction (PCR) in which 75% (18/24) cats and 64% (32/50) backyard chickens were positive. The correlation between IHA and PCR from both animals was considered weak. It was not possible to obtain isolate strains from cats. From the chickens it was possible to obtain 15 isolates strains from which 8 presented tachyzoites (acute toxoplasmosis) and 7 presented brain tissues (chronic toxoplasmosis). After the DNA extraction from the isolates the RFLP-PCR was performed using the following primers SAG1, 5’-3’ SAG2, altSAG, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3. It was possible to define the genotype from 9 isolates. According to ToxoDB seven isolates corresponded to genotype #65 and two isolates have not been described previously. The genetic analysis showed high diversity and variability. The virulence essays showed that the mortality rate in mice in seven isolates that presented tachyzoites, from the same genotype, detected high virulence in 4 isolates and intermediary virulence in 3. The morphometry analysis of the tachyzoites of these isolates showed statistical difference in at least one of the analyzed variables such as length or nuclei-apical complex distance. These phenotypic differences in isolates from the same genotype show the need to the continuity of the genetic characterization of the parasite using other primers which could be related to the isolated phenotype. The study demonstrated the importance of knowledge about the molecular epidemiology of T. gondii in the metropolitan region of Goiânia, which presents high rates of seroprevalence in hosts.Item Identificação de novos fármacos antimaláricos através de estratégia de quimiogenômica por reposicionamento e validação experimental(Universidade Federal de Goiás, 2018-03-05) Rodrigues, Juliana; Cravo, Pedro Vitor Lemos; http://lattes.cnpq.br/1059199347781390; Andrade, Carolina Horta; Andrade, Carolina Horta; Andrade, Éverton Kort Kamp; Castro, Ana Maria; Neves, Bruno Júnior; Costa, Fabio Trindade MaranhãoMalaria is an infectious disease of possible chronic evolution that affects billions of people in the tropics and subtropics. P. falciparum is the most lethal malaria parasite of humans, while P. vivax is the most widely distributed. The effectiveness of the antimalarial treatment is compromised by the ability of the parasite to evolve resistance to the compounds and by the lack of new effective antimalarials, underscoring the urgent need for the discovery of new drugs. One of the strategies that has been gradually explored in the search for new therapies is the so-called "drug repurposing" approach. In this context, the goal of the present study was to use a drug repurposing-chemogenomics strategy to identify effective drugs against malaria parasites. A comparative genomics tool available from the TDR Targets Database was used through to select targets expected to be present exclusively in P. falciparum and P. vivax parasites, but absent in humans. Each of the selected targets was then used as a query in the following databases: Drugbank, Therapeutic Target Database and STITCH. The P. falciparum and P. vivax targets were aligned with their predicted homologue targets, using pairwise BLAST, to compare functionally relevant regions. Only those where ≥ 80% overlap was observed between the two sequences for the corresponding drug target were considered for subsequent studies. Thereafter, the drugs identified were submitted to a bibliographic search to find drugs that were never evaluated against malaria parasites in the past. A prediction of active compounds was performed through binary QSAR models. The selected drugs were submitted to in vitro assays using asexual stages of P. falciparum (strains 3D7, chloroquine-sensitive, and W2, multidrug-resistant). Epirubicin displayed potent in vitro activity against the 3D7 strain (IC50 = 140 nM). In addition, the drug was shown to be about twice as active against the W2 resistant strain (IC50 = 69 nM), exhibiting even greater activity than chloroquine. At present, in vitro experiments in sexual stages (ookinete conversion) and in vivo assays with P. berghei and P. chabaudi are being carried out. In conclusion, epirubicin is a good antimalarial drug candidate, although future studies are required to investigate its mechanism of action, potential toxicity, and eventually, to advance in the drug development process.Item Diagnóstico da hanseníase paucibacilar: resposta imune celular a proteínas recombinantes do Mycobacterium leprae(Universidade Federal de Goiás, 2008-02-28) Sampaio, Lucas Henrique Ferreira; Stefani, Mariane Martins de Araújo; http://lattes.cnpq.br/5581414958714905; Stefani, Mariane Martins de Araújo; Mira, Marcelo Távora; Oliveira, Milton Adriano PelliObjetives: To test T cell effector/memory immune response to M leprae recombinant proteins assessed by IFN-y production among leprosy patients and controls. Methods: The following study groups were assessed: A. Paucibacilary leprosy (PB; n=13); B. Multibacilary leprosy (MB; n=10), PB and MB were newly diagnosed untreated patients; C. Pulmonary tuberculosis, HIV negative (TB; n=10); D. Healthy endemic controls (EC; n=10); E. Multibacilary household contacts (HHC; n=13). The following M.leprae recombinant proteins were tested: ML0091; ML0276; ML0398; ML0541; ML0543; ML0840; ML0953; ML1011; ML1213; ML1623; ML2044 and the fusion proteins ML46f and ML56f at 10ug/ml using Whole Blood Assay (WBA), 450mL/well, 37oC, 5%CO2. After 24 hours incubation, plasma was collected to quantitate IFN-y by ELISA (QuantiFERON CMI, Cellestis, Australia). Results- Based on IFN-y levels, M. leprae recombinant proteins were classified into: A - “Immunogenic and Specific”: ML0276, ML1623, ML0091, ML46f, ML0840, ML2044; B - “Immunogenic and not specific”: ML0398; C -“Not Immunogenic”: ML1011, ML57f, ML0541, ML0316, ML0543, ML1213, ML0953. Conclusions- Plasma IFN-y levels among PB leprosy and healthy household contacts indicated six potential candidates for the diagnosis of PB leprosy represented by the following immunogenic and specific M. leprae recombinant proteins: ML0276, ML1623, ML0091, ML46f, ML0840 and ML2044.Item Desigualdades na epidemiologia do câncer colorretal no Brasil(Universidade Federal de Goiás, 2023-06-07) Schaedler, Anelise Camila; Oliveira, Max Moura de; http://lattes.cnpq.br/7699001066097321; Oliveira, Max Moura de; Malta, Deborah Carvalho; Iser, Betine Pinto MochleckeColorectal cancer (CRC) is among the most common cancers worldwide, affecting over 1,931,590 people annually. Social determinants of health related to the incidence and mortality of colorectal cancer have been analyzed, revealing higher incidence rates in socioeconomically favorable regions, while mortality rates are higher in low- and middle-income regions. Objective: To analyze the incidence, prevalence, mortality, and Disability-Adjusted Life Years (DALY) of colorectal cancer in Brazil and its Federative Units, correlating them with socioeconomic indicators.Methodology: This is an ecological study, with a time series analysis of incidence, mortality, DALY, and prevalence rates of CRC in individuals aged 30 years and older. The corrected and estimated data from the Global Burden of Diseases Study 19 (GBD19) for Brazil, by Federative Unit (FU) and sex, from 1990 to 2019, were used. Agestandardized rates were calculated using the GBD19 standard population. Indicator trends were estimated using the average annual percent change and 95% confidence interval (95% CI) by joinpoint regression. Pearson's correlation was used to correlate the indicators with the Human Development Index, using the Socio-Demographic Index (SDI) obtained from GBD19. Results: The analysis of age-standardized rates per 100,000 inhabitants showed an increase in all states, regardless of sex, from 1990 to 2019, with the following changes: incidence from 23.8 (1990) to 35.48 (2019), prevalence from 167.7 (1990) to 319.98 (2019), mortality from 41.74 (1990) to 49.14 (2019), and DALY from 934.4 (1990) to 1,109.35 (2019). The highest incidence and prevalence trends were observed in the North and Northeast macroregions and among males. Mortality and DALY trends showed predominantly higher rates among men in all Federative Units (except the Federal District) and remained stable among women. Correlation analysis between SDI and CRC showed a positive association with incidence and a negative association with mortality and Disability-Adjusted Life Years. Conclusion: The incidence, prevalence, mortality, and DALY rates of colorectal cancer were found to be high throughout the country, which can be explained by Brazil being a developing nation with increasing urbanization and greater access to risk factors. Rates are predominantly higher in more developed Federative Units where Westernized diets are already prevalent in the food routines of these groups. The high incidence trends in less favored regions may be due to the urbanization process, which is making previously absent risk factors more accessible over the years. Another reason could be the amplification of healthcare assistance in these regions, resulting in an increase in the reporting of new cases. The elevated mortality and DALY trends in socioeconomically disadvantaged regions may be related to the lack of public policies for prevention, screening, and treatment in these specific populations. Based on these findings, this study reinforces the importance of creating and implementing public health policies targeting highrisk groups for prevention and screening. Relevance and Impact: Colorectal cancer is a current public health issue worldwide. This study contributes to the epidemiological update of this cancer in Brazil, highlighting its relevance within the Brazilian context. The findings can contribute to prioritizing public health policies aimed at cancer prevention and control.Item Percepção e adesão de usuários de internet às medidas não farmacológicas para mitigação da Covid-19: uma análise de inquéritos repetidos nos períodos de menor e maior adesão no Brasil(Universidade Federal de Goiás, 2022-12-08) Sérgio, Luciana Madeleine da Silva; Rosa, Michelle Quarti Machado da; http://lattes.cnpq.br/4053156553973596; Toscano, Cristiana Maria; http://lattes.cnpq.br/3772312631884265; Toscano, Cristiana Maria; Oliveira, Max Moura de; Souza, Romulo Cristóvão deIntroduction: Mitigation of the Covid-19 pandemic caused by the new coronavirus (SARS-CoV 2), which started in 2020, required the implementation of a set of public health measures to reduce virus transmission in the population. Prior to the availability of specific vaccines and antiviral drugs, so-called non-pharmacological measures (social distancing, hand sanitization, and mask wearing) were the only measures with the potential to reduce the number of cases, hospitalizations, and deaths from Covid-19. Understanding and comprehension of the importance of these measures, as well as public adherence to them, are therefore key factors for their effective implementation. Little is known yet about the perception, knowledge, and behavior of society regarding the measures advocated and implemented during the Covid-19 pandemic. Objective: To characterize and evaluate, specifically for Brazil, the perception, knowledge, and behavior of Brazilian Internet users regarding Covid-19, including the population's adherence to non-pharmacological measures implemented. Also, we sought to identify factors associated with adherence to non-pharmacological measures among users in two periods identified as periods of lowest and highest adherence to non-pharmacological measures. Method: This is a cross-sectional study with repeated surveys. We used data from a sample of adult users (over 18 years) of the Facebook application who were invited to participate in an international survey conducted by the Massachusetts Institute of Technology (MIT) in collaboration with Johns Hopkins University in the United States. In Brazil the data was collected in the period July/2020 to March/2021. The source of information, knowledge, perception about Covid-19, and population behavior regarding adherence to Covid-19 protective measures were reported by a convenience sample that included recording 63,989 responses from internet users over 19 surveys. The following groups of variables were considered: 1) sociodemographics, 2) individuals' behavior regarding protective measures to Covid-19 (prevention with physical distance, hand sanitization, and mask use), 3) individuals' perception of Covid-19 (risk in the community; risk of infection; infection control; severity of infection; effectiveness of mask use, and effectiveness of hand sanitization), 4) knowledge about Covid-19 (knowledge about risk group and knowledge about transmission), and 5) source of information about Covid-19. Due to the lack of information on independence between waves in the study, two moments (lower and higher adherence of individuals regarding distancing, hand sanitization, and mask use) were selected to be analyzed. The descriptive analysis of the variables presented the frequency estimates and response percentages for the categorical variables, as well as their respective confidence intervals. In order to identify factors associated with greater adherence of the population to the measures, univariable and multivariable analysis was performed through Poisson regression models, with estimated prevalence ratio (PR) and respective 95% confidence intervals (CI). Results: The recorded number of internet user responses in the lowest adherence period (Nov 9 - 14 Nov 23/2020) was 2,339 and the highest adherence period (Mar 15 - Mar 29/2021) was 2,668. The sociodemographic characterization of the users regarding gender, age and education were similar during the two periods, lowest and highest adherence respectively, where the majority were female (53.3% and 53.7%), young (62% and 61.8%) and more than 40% had high school education (47.7% and 48.2%). Most individuals presented an important perception about the risk of Covid-19, where in the period of lowest adherence, 39.4% (330) and highest adherence 55.1% (528) claimed that the risk of the disease to the community is very and extremely dangerous. As for the understanding about the effectiveness of the measures, in the periods of lower and higher adherence respectively, 49.5% (1,094) and 52.4% (1,372) claimed that hand hygiene is extremely effective. Regarding the behavior, during the periods of lower and higher adherence respectively, considering the adoption of protective measures, it is observed that 56.8% (1,394) and 65.1% (1,869) of users reported adherence to physical distancing, 88.9% (2,139) and 94.2% (2,549) used a mask, and 83.3% (2,018) and 86% (2,391) sanitized their hands. Through Poisson regression, it is found that the prevalence in performing the protective measures (distancing, hand sanitization, and mask use) during the period of lower adherence was higher among the young (PR = 1.28; PR = 1.05 and PR = 1.07) and elderly (PR = 1.52; PR = 1.31 and PR = 1.35). For the period of higher adherence, it is observed that young (PR = 1.63; PR = 1.19 and PR = 1.05), elderly (PR = 1.25; PR = 1.33 and PR = 1.00) and individuals with knowledge about the form of transmission (PR = 1.48; PR = 1.62 and PR = 1.06) performed the protective measures more frequently. In addition, it is found that women (PR = 1.02 and 1.18) and individuals with greater understanding about the effectiveness of hand hygiene (PR = 1.16) and mask use (PR = 2.13) did more hand hygiene and used a mask more frequently. Conclusion: Throughout the Covid-19 pandemic in Brazil it is observed that internet users' adherence to non-pharmacological measures may be associated with the epidemiological picture of the disease, in which, the periods of lower (Nov 9 - 14 Nov 23/2020) and higher (Mar 15 - Mar 29/2021) adherence are characterized by lower and higher numbers of reported cases, respectively. Thus, during the periods, there was high adherence to the use of masks and hand washing. The number of individuals who adhered to physical distancing was high, although it could have been higher. A significant perception of risk was observed, as well as levels of knowledge about the effectiveness and importance of the protective measures implemented. However, these values varied in the population according to age and education level. Importantly, greater knowledge about the disease was associated with greater adherence of individuals to non-pharmacological measures implemented as public health strategies. Relevance and impact: The results showed how internet user's knowledge of Covid-19 influenced the adherence of individuals to non-pharmacological measures to face the pandemic. Continuous information dissemination and risk communication to the population are key strategies that should be instituted and prioritized during public health emergencies in order to increase the population's adherence to non-pharmacological mitigation measures. The findings of this study present important points for the Brazilian and international literature, and above all, relevant evidence to support managers and health policies during public health emergencies.Item Estudo da interação plasmodium-hospedeiro via meta-análise de dados públicos de sequenciamento duplo de RNA(Universidade Federal de Goiás, 2023-06-29) Silva, Bárbara Fernandes da; Gardinassi, Luiz Gustavo Araújo; http://lattes.cnpq.br/8922981412535108; Gardinassi, Luiz Gustavo Araújo; Matteucci, Kely Catarine; Fonseca, Simone Gonçalves daMalaria is a potentially fatal disease caused by Plasmodium parasites transmitted by Anopheles mosquitoes. Infection causes an intense inflammatory response, but the molecular mechanisms of interaction between host and parasite remain largely unknown. Although many studies have been dedicated to understanding the host immune response using RNA sequencing of whole blood from malaria patients, technological advances have enabled an integrative evaluation of both host and parasite transcriptomes. The accumulation of different datasets in public repositories provides an opportunity to uncover the interaction between host and Plasmodium via integrative data analysis. Methods. We evaluated whole blood RNA-seq data from 8 different datasets of individuals with malaria caused by infection with Plasmodium falciparum (Pf). The transcriptomes from humans were transformed to Blood Transcriptional Modules (BTM). Spearman´s correlation method was used to evaluated associations between BTMs and Pf genes. PlasmoDB platform was used to evaluate Pf genes. Results Initially, we selected correlations (p < 0.001) conserved in 60% of the datasets to generate a hostparasite transcriptional network composed, for exemplo, of BTMs related to the activation and response of dendritic cells and myeloid cells, besides of leukocyte differentiation and NK cells. Later, we selected correlations conserved in 75% of the datasets, which revealed a host-parasite transcriptional sub network, in which BTMs are related to monocytes, activation of innate immunity, as well as regulation of inflammation. Moreover, the comparison between the activity of BTMs included in this sub network between individuals with malaria and healthy controls demonstrated the downregulation of genes involved with SMAD2/3 signaling and transcriptional targets of glucocorticoid receptor, while the other BTMs were upregulated, including modules related to monocytes and signaling by TLR, RIG-I and cytosolic DNA sensors. Conclusion. In conclusion, our study identified diverse associations between genes of P. falciparum and gene modules reflecting cells and processes of the human immune response that can be prioritized in experimental assays.Item Infecção por Stenotrophomonas maltophilia em hospital terciário em Goiânia: características clínicas e microbiológicas e fatores de risco para letalidade(Universidade Federal de Goiás, 2014-07-08) Silva, Claudia Neto Gonçalves Neves da; Araújo Filho, João Alves de; http://lattes.cnpq.br/3769452101687074; Araújo Filho, João Alves de; Kipnis, André; Pereira, Milca SeverinoStenotrophomonas maltophilia is a Gram-negative bacillus found in natural and in hospital environments. S maltophilia’s high level intrinsic resistence to antibiotics and heavy metals justifies the importance of this bacteria in the nosocomial environment. The aim of this study was to describe the clinical and epidemiological characteristics, and the outcome of infection due to S. maltophilia; describe the antibiotic sensitivity and the genetic relatedness from this bacteria from january 2010 to july 2013 in Hospital das Clínicas – UFG (HC/UFG). Clinical and epidemiological data were obtained from medical records of patients; antimicrobial sensitivity was determined by the methodology Vitek 2®; and the genetic similarity was determined by Pulsed-Field Gel Eletrophoresis (PFGE). Over the study period, 75 isolates of S. maltophilia were indentified in 56 patients, with mean of 1.34 isolates per patient (range 1-8). Among the total, 34.6% were in the clinical intensive care unit, and 17.3% in the clinical infirmary. The 3 intensive care unit accounted for 56% of all isolates. The mean age of patientes was 45.75 years (range 0-83 years), and 51.8% were male. All cases of infection were considered health care-associated. The most comom coexisting condition were haematological malignancy, in 18 (32.1%) patientes. The most frequent clinical manifestations was fever in 47 (83.9%) patients, and leukocytosis or leukopenia in 31 (55.4%). Invasive devices were frequently used, and central venous cateter, and mechanical ventilation were the most. Most patients (94.6%) used broad spectrum antibiotics before the acquisition of the bacteria. The most frequent type of infection was ventilator-associated pneumonia (42.9%) followed by cateter-associated bacteremia (16.1%). Trimethoprim-sulfamethoxazole was the treatment of choice in 32 (57.1%) patients. The outcome of infection due to S. maltophilia was infection-related death in 62.5%. The risk factors related to mortality in this study were shock, acute renal failure, use of mechanical ventilation, use of urinary device and feeding tube. Trimethoprim-sulfamethoxazole resistance, the treatment of choice for infection by S. maltophilia, and levofloxacin resistence were detected in HC / UFG. Twenty isolates were assessed for genetic variability by pulsed-field gel eletrophoresis (PFGE) with observation of high variability between strains. We conclude that S. maltophilia is a nosocomial bacteria of clinical importance mainly due to high associated mortality, and few therapeutic options. Resistance to trimethoprim/sulfamethoxazole was low, but must be monitored in HC / UFG.Item Reposicionamento de fármacos para Leishmania spp: estratégias “in silico” e avaliação experimental(Universidade Federal de Goiás, 2015-08-04) Silva, Diego Cabral; Andrade, Carolina Horta; http://lattes.cnpq.br/2018317447324228; Andrade, Carolina Horta; Cravo, Pedro Vitor Lemos; Silva Junior, Floriano PaesLeishmaniases are diseases caused by more than 20 protozoan parasites belonging to the genus Leishmania, and transmitted through the bite of infected female Phlebotomine and Lutzomya. An estimated 20,000 to 30,000 deaths and 1.3 million new cases occur annually. Currently available drugs have serious limitations regarding their efficacy and especially their toxicity, side effects and costs. Thus, there is a pressing need for new therapies that are safer and more effective. Due to the high costs of traditional process of drug discovery and development, alternative strategies have been developed to speed up this process, and reducing its costs. Among them, is drug repositioning, which is the discovery of new therapeutic applications for drugs already on the market. The aim of this work was to search and identify approved and clinically available drugs with potential antileishmanial activity, using bio- and cheminformatics approaches, and experimental validation of these drugs using in vitro assays. Initially, we generated a dataset of Leishmania genes with orthologs in four species (L. major, L. braziliensis, L. infantum e L. mexicana), specific to the genus Leishmania, trying to explore potential molecular targets that could be effective against all species and essential to the process of development and differentiation of the parasite. This dataset was used to interrogate three databases of approved drugs (DrugBank and TTD) aiming to identify homologues of validated targets for other diseases. Furthermore, binary QSAR models were generated from phenotypic assay data, using different descriptors, and two machine learning methods, and then consensus models were built. Homology search allowed the identification of 36 new potential molecular targets that need to be validated experimentally, and 122 drugs. Of these 122 compounds, 28 were previously reported on the literature as actives. Five drugs not yet tested were selected for biological screening in vitro against promastigotes (lansoprazole, ibuprofen, sertaconzole, nilutamide and clomifen). Three of them showed activity at 100 μM and we determined their IC50. Ibuprofen showed an IC50 of 55.08 μM, sertaconazole IC50 < 15 μM and clomifen (IC50 5,75 μM, more potent than the standard drug (pentamidine IC50 = 7,24), suggesting a potential activity. Besides that, the QSAR models generated had adequate statistical parameters, especially for consensus models. One of the models generated by consensus was employed to predict the activity of the drugs identified by the bioinformatics approach. The best models can be used as filters in a virtual screening process. In vitro assays in the promastigote form of L. amazonensis were standardized, and used to successfully identify new potential candidates for drug repositioning.Item Soroprevalência de COVID-19 e infecção por toxoplasma gondii e fatores associados à ocorrência de lesão renal aguda em pacientes coinfectados(Universidade Federal de Goiás, 2022-06-06) Silva, Wania Gonçalves da; Amancio, Ana Laura de Sene; http://lattes.cnpq.br/8039224852182884; Castro, Ana Maria de; http://lattes.cnpq.br/9232309971000621; Castro, Ana Maria de; Storchilo, Heloisa Ribeiro; Freitas, Nara Rúbia deTitle: Seroprevalence of COVID-19 and Toxoplasma Gondii Infection and Factors Associated with the Occurrence of Acute Kidney Injury in Coinfected Patients. Introduction: Coronavirus 2019 (COVID-19) is a severe acute respiratory infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), considered one of the greatest global public health challenges, contributing to a high burden of disease and death. in different age groups, in addition to those with comorbidities, such as cardiopulmonary and renal diseases, neoplasms, among others. Faced with the global SARS-CoV-2 pandemic and the high prevalence of Toxoplasma gondii infection in the population, studying the factors associated with the occurrence of acute kidney injury in COVID-19 patients, and the seroprevalence of T. gondii infection becomes necessary, to provide support for adequate initial assessment, improvement in prognosis and prevention strategies for the control of Covid-19 and Toxoplasmosis. Objective: To epidemiologically characterize a group of inpatients in an intensive care unit with COVID-19, coinfected with T. gondii. Methods: Analytical cross-sectional study, with an analytical approach, carried out in a public university hospital in Goiânia - Goiás. A total of 314 patients participated in the study, admitted to an intensive care unit, diagnosed with COVID-19 by the RT-PCR or RTLAMP methods, and serology for toxoplasmosis by the IgG ELISA method, and IgM Results: A study of seroprevalence of toxoplasmosis in patients with COVID-19 was not identified. Seropositivity was found for the IgG class: 78.1%, and IgM/IgG: 3.5%. In the sample, there was a predominance of males, ie (55.4%). Most of them are people aged 60 years or older (49.7%), living in Goiás (98.5%), which represents the highest frequency of hospitalizations among elderly people. 20-39 years (p=0.014) and 40-59 years (p=0.027) were protective factors for toxoplasmosis in patients with COVID-19. 88.5% of mixed race, living with someone (65.6%), retired (38.6%), with income of less than 2 years minimum (51.8%), with incomplete elementary school (53.4%) and living with 3 residents (52.3%). It is observed that there is an inverse trend, the higher the level of education, the lower the prevalence of toxoplasmosis. For behavioral habits (alcohol consumption, smoking, use of illicit drugs), no statistically significant was found. As for the origin of origin (67.9%) from a Basic Health Unit. As comorbidities prevail, systemic arterial hypertension, diabetes mellitus, obesity, COPD, neoplasms, and clinical manifestations, fever, asthenia, myalgia and headache. As for death, 158.5 % died. There were no significant differences between patients and patients who were discharged or downloaded. There were no significant results associating Acute Renal Failure (AKI) with toxoplasmosis in patients with COVID-19. Patients with COVID-19 and evidenced the presence of kidney disease 9 who are hospitalized in patients, evolution of the clinical condition to hemodialysis (P) No statistically significant results were identified that show associations between variables, a factor that demands further investigation. Conclusions: The results of the study show a elevated prevalence of toxoplasmosis patients at susceptibility to COVID-19 (77%), suggesting a high susceptibility of patients with developmental disease, evidencing opportunistic opportunities. Individuals over 60 years of age are at greater risk of being co-infected. There were no ARI, associations between toxoplasmosis infection in patients with COVID-19 and patients with COVID-19, who have more kidney problems and consequently progress to hemodialysis. Relevance and impact: It is valid, due to the high frequency of Toxoplasmosis and COVID-19 cases and association with renal impairment of patients who evolved with the need for renal replacement therapy, it is important to reflect and understand the severity of the disease, establish preventive, diagnostic and effective therapies in a more targeted and faster way, as well as preventive measures for effective decision making.