Avaliação da produção de BMP2 no intestino de camundongos infectados com Trypanosoma cruzi
Nenhuma Miniatura disponível
Data
2020-03-12
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
One of the main late complications of Chagas' disease (CD) is the megacolon, affecting
approximately 10% of symptomatic patients. However, studies are needed to understand
mechanisms involved in the progression of this condition in the chronic phase of CD.
Myenteric plexus neurons are known to be essential for the control of intestinal motility.
Through infection by Trypanosoma cruzi (T. cruzi), the inflammatory profile that sets in is
involved in neural destruction. One of the proteins related to the maintenance of nerve cells in
the myenteric plexus is the type 2 morphogenetic protein (BMP2), produced mainly by muscle
macrophages. The homeostasis of the BMP2/macrophage ratio is directly involved with
intestinal motility and the maintenance of organ function. Thus, the aim of this study was to
correlate the production of intestinal BMP2 with the production of cytokines and
histopathological changes in C57Bl / 6 mice. infected by the T. cruzi Y strain in the periods of
30 and 90 days of infection. The mice were infected with 1000 blood trypomastigote forms.
After the infection period, the mice were euthanized and the spleen and intestine were
collected. The intestine was divided in two, one fragment was used for histological analysis
and the other for quantification of TNF-α, IFN-γ, IL-10 and BMP2, as well as the spleen.
Infection with strain Y induced an increase in the production of TNF-α, IFN-γ and BMP2 in the
intestine after 30 days of infection, as well as an increase in the inflammatory infiltrate and a
decrease in the number of neurons in the myenteric plexus. Collagen deposition increased
gradually throughout the infection, demonstrated at 90 days of infection. It was observed that
the increase in BMP2 after 30 days of infection has a positive correlation with the increase of
IFN-γ and TNF-α in the intestine. However, BMP2 and IFN-γ showed a negative correlation
with the number of neurons in the myenteric plexus in the same period in the organ. As a first
report of the alteration of BMP2 production after infection by T. cruzi, it is suggested that this
imbalance may represent a new pathway in maintaining the intestinal pro-inflammatory
profile, as well as being related to the neuronal damage that the infectious process
establishes.
Descrição
Palavras-chave
Citação
CARMO NETO, J. R. Avaliação da produção de BMP2 no intestino de camundongos infectados com Trypanosoma cruzi. 2020. 62 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2020.