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Item Administração transdérmica de cloridrato de raloxifeno encapsulado em nanopartículas lipídicas: estudos de pré-formulação para seleção dos lipídeos e tensoativos(Universidade Federal de Goiás, 2017-09-26) Alves, Guilherme Liberato; Taveira, Stephânia Fleury; http://lattes.cnpq.br/0382450621383005; Taveira, Stephânia Fleury; Marreto, Ricardo Neves; Gelfuso, Guilherme MartinsIntroduction: Raloxifene hydrochloride (RLX) is a widely prescribed drug for the treatment of osteoporosis and breast cancer. However, RLX presents a low oral bioavailability (2%), being an excellent candidate for transdermal drug delivery. The aim of this work was to develop nanostructured lipid carriers (NLC) containing RLX for transdermal drug delivery. In order to choose proper excipients, a compatibility study was performed. Methodology: Compatibility study was performed with differential scanning calorimeter (DSC), thermogravimetric anaysis (TGA), derivative thermogravimetry (DTG), isothermal stress testing (IST) and solubility study. RLX quantification was performed by high performance liquid chromatography (HPLC). After choosing the excipients, CLN were obtained by the microemulsion technique. Characterization was performed for mean diameter, polidispersivity index (PdI), zeta potential, entrapment efficiency (EE%) and stability. In vitro release and permeation studies were performed in Franz diffusion cells. Results and discussion. Conclusions: Drug-excipient compatibility studies allowed the development of stable and monodisperse CLN, capable of controlling drug release and increase its permeation in the skin when compared to the non-encapsulated drug in the control formulation.Item Estratégia associando métodos substitutivos à experimentação animal para avaliação da toxicidade ocular, categorização e rotulagem de formulações agrotóxicas comercializadas no Brasil(Universidade Federal de Goiás, 2018-11-20) Andrade, Wanessa Machado; Valadares, Marize Campos; http://lattes.cnpq.br/615775524316701; Valadares, Marize Campos; Costa, Renata Mazaro; Paula, José Realino de; Gomes, Thaisângela Rodrigues Lopes e Silva; Gomes, Marcelo do NascimentoIntroduction: Different strategies for categorization and labeling (C & L) of ingredients and finished products have been considered by the Regulatory Agencies. Regarding agrochemical formulations, the lack of global harmonization for C & L is the main problem for the implementation of substitute methods. On the other hand, in vitro and ex vivo methods are successfully adopted to assess the potential toxicity of chemicals. The standard Bovine Cornea Opacity and Permeability (BCOP) test provides information on permeability and opacity, but histomorphometric analysis of the cornea has been recommended as an additional parameter to better categorize irritant products for the eyes. Objective: To suggest a platform, associating in vitro and ex vivo methods, based on mechanistic parameters of ocular toxicity evaluation for C & L of agrochemical formulations commercialized in Brazil. As well as measuring the depth of bovine corneal injury induced by eye irritants from all categories of the United Nations Globally Harmonized System for Classification and Labeling of Chemicals (UN GHS) and associating it with the severity of the damage. Material and Methods: For the platform, 86 agrochemical formulations of different categories of ocular toxicity were selected in the categorization system of the National Agency of Sanitary Surveillance (ANVISA). First, the potential for ocular toxicity was assessed through the cytotoxicity endpoint using the Short Time Exposure (STE) test. In the second step, the non-classified samples and those classified as non-irritating by the STE were evaluated for the opacity, permeability and histopathological changes of the cornea through the BCOP test. The third step was to evaluate the potential for induction of vascular damage through coagulation and hemorrhage outcomes using the Hen egg Chorion-allantoic Membrane test (HET-CAM). For proficiency, 12 substances belonging to the categories UN GHS (Category 1, 2A and 2B) were selected. The BCOP assay was performed to determine the in vitro irritation index (IVIS). After the fluorescein permeability assay, the corneas used were sectioned in a 5 μm thick cryostat, stained with Hoechst 33342 and analyzed by fluorescence microscopy. Five measurements of depth of permeation of fluorescein were obtained in relation to the total thickness of the epithelium. Results and Discussion: From the palataform, of the 86 formulations of agrochemicals, after the STE test, 68 were highly cytotoxic and were categorized as Category 1 by UN GHS; 13 not classified and 5 non-irritating. These 18 agrochemical formulations were evaluated at the second level of the platform by the BCOP test followed by histopathological analysis, and the irritation profile of these agrochemical formulations was confirmed by the HET-CAM assay. Thus, 7 presented opacity, damage to the cornea in the epithelial portion and in the stroma, coagulation and hemorrhage, and therefore were categorized in Category 2 A of the UN GHS. 11 caused only coagulation and hemorrhage and were categorized into Category 2 B of the UN GHS. In the proficiency, the results showed that the permeation depth of fluorescein was directly proportional to the degree of irritation of the substances tested. Conclusion: Since C&L of agrochemical formulations in Brazil is not based on the UN GHS, this tier platform could be useful to harmonize the categorization of agrochemical formulations in Brazil, providing regulatory subsidies for ANVISA. As well as, refinement of BCOP by analysis of lesion depth through epithelial permeation of fluorescein can be considered a useful quantitative parameter to better categorize eye irritants.Item Perfil epidemiológico e fatores associados à síndrome respiratória aguda grave em Goiás, no período de 2013-2018(Universidade Federal de Goiás, 2020-09-02) Araujo, Kamilla Lelis Rodrigues de; Silva, Lara Lívia Santos da; http://lattes.cnpq.br/3374527511007494; Ternes, Yves Mauro Fernando; http://lattes.cnpq.br/3417780592064501; Silva, Lara Lívia Santos da; Reis, Angela Adamski da Silva; Siqueira Júnior, João BoscoSevere Acute Respiratory Syndrome (SARS) is a condition that, in addition to classic symptoms of influenza, shows signs of worsening with high morbidity and mortality. Thus, is a major concern for public health, being a disease of epidemiological importance, thatmust be notified and investigated. Therefore, the aim of this study was analyze the epidemiological profile and associated factors of SARS reported in the state of Goiás, between 2013 to 2018. This is a retrospective cohort study, with data obtained from the National disease notification system - Influenza Web. Logistic regression analysis was performed to verify the association between death and ICU admission and exposure variables by using the SPSS 25.0 statistical package program. Between 2013 and 2018, 4,832 cases of SARS were reported in Goiás. During the analyzed period, the majority of cases was attributed to Influenza virus (A or B), mainly Influenza A (22.3%), with predominance of pandemic H1N1 Influenza 2009 subtype H1N1pdm09 (17,9%). Among the variablesrelated to ICU admission, longer time to start antiviral treatment in days wasassociated with an increase in hospitalization while the absence of previousinfluenza vaccination, longer time to start antiviral treatment and older age wereassociated with deaths. Thus, this study showed a high frequency of SARS in the state of Goiás between the years 2013 and 2018, with the Influenza A virus(H1N1pdm09) being the main etiological agent. Among the factors related toadmission to the ICU and death, the time of initiation of treatment with antiviral, vaccination status and age of the patientwere observed, which highlights theneed for constant monitoring of the epidemiological situation of this disease, inorder to adopt public health measures for prevention and control.Item Utilização do óleo obtido de resíduos agroindustriais de goiaba (Psidium guajava L.) na nutrição de poedeiras comerciais(Universidade Federal de Goiás, 2014-10-31) Barbosa, Nathalia Pedroso; Stringhini, Jose Henrique; http://lattes.cnpq.br/8505634095383289; Mello, Heloísa Helena de Carvalho; http://lattes.cnpq.br/5510965166352073; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510; Conceição, Edemilson Cardoso da; Bara, Maria Teresa Freitas; Café, Marcos BarcellosPsidium guajava L. is a plant originated in Mexico, belonging to mytarceae family. The guava fruit is growth in economic value for the production of derivatives in the Brazilian agribusiness. However the use of guava pulp generates waste discarded in the environment, without the correct use, ie an environmental liability. The guava residue consists of pulp, peel, but mainly seeds. Yet is not described in the literature depth studies on its chemical composition or a suitable waste recovery. Thus, this study aimed to characterize the dust of organic residues of guava (donated by Food Predilecta ® - Matão, São Paulo), followed by obtaining the ethanol extract of the percolation method. The ethanol extract was characterized as the fatty acid profile, density and acid number and came to be known as: Powder oil waste processing Guava (OWG) from the OWG obtained the volatile fraction by hydrodistillation and characterization was carried out by gas chromatography coupled to mass spectrometry (GC / MS), followed by characterization of β-caryophyllene marker for high performance liquid chromatography (HPLC) and the identification of ORG constituents by GC / MS. We evaluated the antioxidant potential by free radical trapping methods 2,2-diphenyl-1- picryl hydrazyl (DPPH) and the capture of ferrous sulfate (FRAP). And finally, evaluated the implementation of the OWG, in laying hens Bovans White to verify the performance, posture, egg quality, digestibility and chemical composition of eggs and lipid oxidation of gems. The analysis of the volatile fraction of the OWG by GC / MS resulted in the identification of 25 compounds having as the major, the β-caryophyllene with 21.15%. Thus, an analytical HPLC method was revalidated in accordance with the criteria of Resolution 899/03 of ANVISA for quantification of β-caryophyllene (26.26%) in the OWG, to then be used for monitoring and characterization of OWG. Moreover, GC / MS, were identified in OWG, vitamin E and phytosterols such as α- tocopherol and the β-tocopherol, β-sitosterol and campesterol. OWG showed potential antioxidant activity, with EC50 31.24 mg / mL for the capture of DPPH and a content of 3,67.10-6 mM of ferrous sulfate per gram of capture OWG. Performance reviews and posture did not differ between treatments. The eggs of the birds supplemented with ORG and BHT had favored characteristics in relation to egg weight, yolk weight, White egg weight, suggesting the replacement of BHT, a commercial antioxidant with potential carcinogen by OWG. In supplementation in the diet digestibility analysis of OWG and BHT favored the absorption of protein and feed lipids, suggesting that because the oil antioxidant potential in preserving the laying of the power of nutritional constituents as well as to preserve the moisture content, low value caloric albumen and the lipid content in the yolks. The results showed an egg production with superior quality compared to conventional production. The work generates natural antioxidant additive application alternatives that results in improved quality, productivity and reduction in production costs. Moreover favors the export in view of the new requirements of importing countries products of Brazilian poultry.Item Avaliação da eficácia do nusinersena (Spinraza®) no tratamento de atrofia muscular espinhal: uma revisão sistemática(Universidade Federal de Goiás, 2019-11-01) Borges, Stéfani Sousa; Itria, Alexander; http://lattes.cnpq.br/7278290457268315; Itria, Alexander; Reis, Angela Adamski da Silva; D’Alessandro, Walmirton Bezerra; Suzuki, Karina; Tobias, Gabriela CamargoSpinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by mutations or deletions of the survival motor neuron 1 (SMN1) gene, which leads to degeneration of motor neurons of the spinal cord. Nusinersen, commercially known as Spinraza®, has been approved and incorporated into the Brazilian health system as the first pharmacological treatment for SMA, however, a number of ethical issues have been raised, indicating the need for further studies to confirm the drug’s efficacy. In view of this, the objectives of this study were to assess the efficacy of nusinersen (Spinraza®) in SMA’s treatment through a systematic review of randomized controlled trials (RCTs) and cohort studies, and to evaluate the methodological quality of the included studies. The identification and selection of the studies were conducted by two reviewers, blinded and independent in following databases: Cochrane Library, Embase, Lilacs (via Virtual Health Library) and Medline (via Pubmed). An additional search in references of the included articles and in the international database Clinical Trials was conducted to capture as many publications as possible. As inclusion criteria, were selected the full published full articles of human studies whose objective(s) was to evaluate the efficacy of nusinersena in SMA’s treatment. For quality assessment and bias identification, included RCTs were assessed according to the CONSORT (Consolidated Standards of Reporting Trials) Recommendation and cohort studies in accordance with STROBE (Strengthening the Reporting of Observational Studies in Epidemiology) Recommendation. The search in the databases returned 427 publications, of which 112 were removed by duplication and 309 excluded because they did not meet any of the inclusion criteria, totalizing six publications included in this review, of which two RCTs and four cohort studies. The total population of the analyzed articles yielded 461 both sexes individuals and diagnosed SMA Types I, II or III. The most used motor function improvement rating scales among the studies were HINE-2 and CHOP INTEND. The efficacy of nusinersen (Spinraza®) was compared between studies taking into account the research design, population included, and outcomes identified based on the progress of motor scale scores. The validity of the outcomes was discussed according to the quality of each publication and the risks of biases identified under the CONSORT and STROBE Recommendations. Financial support from the drug-producing industry was identified as the main bias in some of the studies. Scientific evidence has shown a significant improvement in motor function in patients treated with nusinersena, although the combination of the outcomes discussed in this review are not considered sufficient to confirm an increase in patient survival. It was identified that an improvement in the patterns of conduct and reporting of studies included in the review are important attitudes to be adopted.Item Extrato líquido padronizado em capsantina a partir de Capsicum chinense Jacq. (Variedade Biquinho) e suas aplicações no desenvolvimento de um produto cosmético anti-aging(Universidade Federal de Goiás, 2018-12-13) Brito, Lucélia de Sousa; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510; Conceição, Edemilson Cardoso da; Borges, Leonardo Luiz; Silva, Luis Antônio DantasIntroduction: The pepper Capsicum chinense Jacq., popularly known as "biquinho pepper", has been outstanding in Brazil for presenting low pungency and a strong aroma. Its fruits are composed of several secondary metabolites, especially carotenoids, with important antioxidant properties. Objective: Develop a cosmetic product from the liquid extract of Capsicum chinense Jacq. standardized on capsanthin, evaluate its anti-aging efficacy in vivo and antioxidant activity. Methodology: The fruits of the "biquinho pepper" were sanitized, weighed, dehydrated, ground in a knife mill and hammers and stored at room temperature. The physico-chemical characterization of the pulverized vegetable raw material was carried out using the methods of microscopic analysis of the powder, determination of the volatile content, total ash, granulometric distribution and swelling index. Afterwards, the raw material was applied in the preparation of the liquid extract, using the techniques of maceration, percolation and concentration by forced ventilation, using the ethyl alcohol 96o GL as solvent extractor. The liquid extract was then characterized for solids content, pH, density and viscosity. Subsequently, total phenol concentration was determined by the technique of complexation of the phenolic compounds with ferric chloride solution, and also, the assay of total flavonoids and carotenoids by spectrophotometric methods. In addition, an analytical method by HPLC was validated for the quantification of the capsanthin in the concentrated liquid extract. Two cosmetics formulations were developed to perform comparative efficacy tests, a phytocosmistry developed from the liquid extract standardized on capsanthin, and another placebo formulation composed only of the galenic base of the product, without the addition of the extract. The cosmetics formulations were then evaluated for their accelerated stabilities and clinical efficacy anti-aging in vivo, evaluated in humans. The antioxidant activity was determined for the vegetable raw material, standardized liquid extract in capsanthin and the cosmetic formulation, using the DPPH radical capture, β-carotene / linoleic acid capture and ABTS radical capture assays. Results and discussion: For the physical-chemical characterization of the pulverized vegetable raw material, the microscopic analysis of the powder demonstrated the intimate nature of the sample as well as its purity, presented a volatile content of 4.62% (m / m), total ash content of 6.341%, the determination of the granulometric distribution classified the sample as coarse powder and the intumescence index was 1.27 ml ± 0.12 using alcohol 96o GL as an intumescent agent. In the physical-chemical characterization of the liquid extract the solids content was 19.16%, pH 3.7 ± 0.06, density 1.040 ± 0.0014 g / mL and viscosity of 3.22 mPas. The total phenolics, flavonoids and carotenoids contents for the raw material and concentrated liquid extract samples were respectively: 18 mg / 100 g and 168 mg / 100 g phenols, 10 mg / 100 g and 90.7 mg / 100 g flavonoids, 10mg / 100g and 83,70 mg / 100g of carotenoids. The validation of the analytical method by HPLC used in the quantification of capsanthin proved to be selective, linear (in the range of 0.48 to 7.2 μg / ml for the capsanthin standard and 26.93 to 404 mg / ml for the liquid extract), accurate, exact and robust. The content of capsanthin found in the liquid extract of C. chinense Jacq. was 1.126% (m / m). Both the standardized liquid extract and the cosmetic formulations were stable against accelerated stability tests, with results of organoleptic and physico-chemical characteristics within the parameters established for the tests. The analysis of the clinical anti-aging efficacy in vivo for cosmetic formulations has demonstrated the potentiation of efficacy for the cosmetic formulation consisting of the standardized liquid extract of Capsicum chinense Jacq., Compared with the placebo cosmetic, showing improvements in wrinkles, expression lines and sagging skin. The antioxidant activity in vitro for the vegetable raw material, standardized liquid extract in capsanthin and cosmetic formulation were respectively: 194.6μg / ml, 73.7μg / ml and 100μg / ml for capturing the DPPH radical relative to the IC50, 33.03%, 42.08% and 19.35% for inhibition of β-carotene oxidation, 22.87 μM Trolox / g, 51.81 μM Trolox / g, 900 μM Trolox / g for the ABTS radical capture. The values obtained for evaluations of antioxidant activities in vitro indicated that all the samples showed to be effective by antioxidant action, although the results have been lower if compared to their respective positive controls. Conclusions: The cosmetic product developed showed antioxidant activity and intended anti-aging efficacy, proving of potential use of the standardized liquid extract of biquinho pepper (Capsicum chinense Jacq.) in technological and innovative phytocosmetic formulations.Item Avaliação do processo eletroquímico na degradação de amoxicilina e determinação de toxicidade dos subprodutos gerados(Universidade Federal de Goiás, 2019-03-22) Caetano, Marcos Pereira; Gil, Eric de Souza; http://lattes.cnpq.br/3218622824233303; Gil, Eric de Souza; Garcia, Luane Ferreira; Comalti Júnior, FlávioIntroduction: Amoxicillin (AMX) is one of the world's most widely prescribed antibiotics for the treatment and prophylaxis of bacterial infections, in both human and veterinary medicine. The incomplete removal of this drug during the treatment of wastewater has attracted the attention of several researchers due to the risks of its bioaccumulation and consequent bacterial resistance. The electroemediation of drugs is of great value in the environmental scope, since electrochemical methods have several advantages when compared to the tools commonly used in the treatment of pharmaceutical and urban effluents. Objective: To evaluate the efficiency of the electrochemical process in the degradation of AMX using different electrolytic media and electrodes, as well as to investigate the adverse effects of the antibiotic before and after the electrochemical remediation. Methodology: The electrochemical tests were performed in three electrolytic media (tap water, Na2SO4 or NaCl solutions) and three anodes (carbon [C], titanium [Ti] and carbon modified with titanium oxide [TiO2 @ C]). The samples analyzed before and after the electro-oxidation were monitored by UV-visible, mass spectrometry and differential pulse voltammetry. After the electrooxidation process, the treated AMX solutions were analyzed by the ecotoxicity test with Danio rerio (zebrafish; paulistinha) and the minimum inhibitory concentration was calculated to determine the antimicrobial potential after treatment. Results and discussion: The titanium oxide modified carbon anode [TiO2 - C]) showed high efficiency, leading to the complete removal of amoxicillin in less than 10 minutes, in the order: [TiO2 @ C]> [Ti]> [C]. The dose of the electrolytes is supported in the following order: 0.1 M NaCl> Na 2 SO 4> 0.01 M NaCl> tap water. Amoxicillin does not pulse cardiac impressive of zebrafish during long term exposure. Conclusions: The electrooxidation of amoxicillin using TiO2 electrode in 0.01 M NaCl solution was able to decrease the antimicrobial activity of amoxicillin. At a high concentration of electrolyte, there is an increase in the rate of drug removal, there is why it is not the habitat that the zebrafish has become a toxic environment. The technique has been serous in the degradation of amoxicillin and can be applied to other organic pollutants.Item Estudo da porosidade de comprimidos de Cloridrato de Propranolol a partir do processamento de imagens de microscopia eletrônica de varredura por elétrons retroespalhados(Universidade Federal de Goiás, 2021-12-07) Camargo, Henrique Santiago de; Gonçalves, Cristhiane; http://lattes.cnpq.br/3935775322457150; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510; Conceição, Edemilson Cardoso da; Silva Júnior, Arnóbio Antônio da; Marreto, Ricardo Neves; Soares, Luiz Alberto Lira; Costa, Roseane Maria RibeiroThe porosity of solid pharmaceutical forms plays a fundamental role in the drug's disintegration the dosage form and consequently in the bioavailability of the active, ensuring its therapeutic action in the desired time, in addition, the porosity influences other aspects such as the mechanical strength of the tablet. Understanding and having methods to study and manipulate the porosity of pills is a crucial factor to have a quality pharmaceutical form. Objectives: Thus, the objective of this work was to study the porosity of Propranolol Hydrochloride tablets from 5 (five) laboratories by processing images obtained in a scanning electron microscope and to correlate these results with dissolution studies and the composition of each tablet. Methodology: The tablets were characterized by thermal techniques, Scanning Differential Calorimetry (DSC) and Thermogravimetry (TGA), and were submitted to hardness, friability and dissolution tests. Electron backscatter SEM images were obtained and processed in order to obtain an estimate of the surface and fracture porosity. Results and discussion: The tablets showed variations in their internal porosity and fracture, all had friability and hardness data that meet current regulatory standards, for the dissolution study, all laboratories presented data within the acceptance criteria of regulatory standards. Conclusions: Through the processing of scanning electron microscopy images obtained from propranolol hydrochloride tablets, it was possible to estimate the surface and fracture porosity of these tablets, thus, this technique is shown as an alternative for the study of the porosity of solid dosage forms (tablets) , being possible to observe the morphology and characteristics of the pores.Item Aplicação do modelo microbiano do metabolismo animal ao estudo da biotransformação de um potencial agente cardioativo(Universidade Federal de Goiás, 2011-02-28) Carneiro, Emmanuel de Oliveira; Oliveira, Valéria de; http://lattes.cnpq.br/6300240031300604; Oliveira, Valéria de; Porto, André Luiz Meleiro; Andrade, Carolina HortaDrug metabolism, or biotransformation, is the enzymatically catalyzed conversion of a drug to a chemically distinct product, known generically as metabolite. The biotransformation impact on drugs biological fate makes essential to study it in the early stages of drug development. Traditionally, in vivo metabolism are conducted in animal models, whose biological fluids are examined for metabolites identification. The observation that simple microorganisms can mimic most of the phase I and some phase II reactions performed in mammals has shown they represent an alternative to produce high amounts of metabolites in vitro. The main organisms used are filamentous fungi, with particular attention to Beauveria bassiana and the genus Cunninghamella. The aim of this work is the application of B. bassiana as a microbial model to study LASSBio-294 biotransformation profile in parallel to an animal model. This substance has demonstrated significant positive inotropic and vasodilatory properties, turning it a potential cardioactive prototype. A capsule with LASSBio-294 was administered to a dog in a pilot study and analyzed by High Performance Liquid Chromatography with Ultraviolet detection (HPLCUV). B. bassiana ATCC 7159 incubation supernatant samples were taken and analyzed by HPLC- UV. At the end of the process, formed metabolites were extracted and purified. One product was characterized by nuclear magnetic resonance (NMR) and mass spectrometry (MS). According to the HPLC-UV analysis, B. bassiana ATCC 7159 produced a major metabolite detected in higher concentration after 24 hours of incubation. This metabolite was the same detected in dog serum samples. The biosynthesis of this metabolite resulted in a yield of 6%. The spectral data show the produced metabolite by dog and B. bassiana ATCC 7159 is a LASSBio-294 sulfoxide derivative. In vitro biosynthesis allowed to obtain, in considerable yield, the main mammalian metabolite detected in the in vivo method.Item Bioconversão e degradação da venlafaxina em seu metabólito ativo(Universidade Federal de Goiás, 2010-03-03) Carneiro, Wilsione José; Oliveira, Valéria de; http://lattes.cnpq.br/6300240031300604; Oliveira, Valéria de; Nóda Perez, Caridad; Cunha, Luiz Carlos da; Menegatti, RicardoThe venlafaxine, 1-[2-dimethylamino-1-(4-methoxyphenyl)-ethyl] cyclohexanol, is a antidepressant drug of second generation. This is one of the most potent reuptake inhibitor of serotonin and noradrenaline, and its therapeutic effect is attributed to this activity. Venlafaxine is biotransformed in the liver to O-desmethylvenlafaxine (desvenlafaxine), N, O-desmethylvenlafaxine, N-desmethylvenlafaxine. Enzymes CYP2D6, CYP2C19 and CYP2C9 metabolize venlafaxine and major metabolite is the O desmethylvenlafaxine. This is pharmacologically active and contributes significantly to the pharmacological effect of venlafaxine, as is found in plasma at high concentrations. The investigation of the formation of degradation products is of great importance, because the products formed may be less active, more active or toxic. Bioconversion or the application of microbial models is a strategy to mimic the mammalian metabolism produces significant quantities of metabolites for studies of pharmacological activity and toxicological. This work represents a forced degradation study of venlafaxine extended release capsules in different stress conditions (acid and alkaline hydrolysis, oxidative and thermal) and select strains of filamentous fungi, to identify those able to metabolize venlafaxine and produce in a semi-preparative major metabolites. The filamentous fungi used were: Aspergillus candidus ATCC 2023, Beauveria bassiana ATCC 7159, Cunninghamella echinulata ATCC 9244, Cunningamella elegans ATCC 6169, Mortierella isabelina ATCC 1757 and Rhizopus arrhizus ATCC 11145. Was developed and validated method stability indicating HPLC using reverse phase for the analysis of venlafaxine in pharmaceutical formulation. The metabolites prepared by bioconversion were used for structural elucidation and later as a reference chemical in the analysis of stability studies and future studies of pharmacological and toxicological activity. The fungus Cunninghamella elegans ATCC 6169 was selected and produced O desmethylvenlafaxine (desvenlafaxine) and dihydroxy-venlafaxine, similar to those found in mammals, reinforcing the application of microbial models for the study of animal metabolism. The study indicated the stability of the acid condition of venlafaxine, formed two degradation products, the products found were similar to those obtained by bioconversion. The O-desmethylvenlafaxine, corresponds to the active metabolite of venlafaxine in humans and was recently approved for the treatment of major depressive disorder. Those studies, one can get the O-desmethylvenlafaxine in bioconversion reactions by Cunninghamella elegans ATCC 6169 and forced degradation studies of venlafaxine. The method developed and validated HPLC method was considered indicative of stability analysis of venlafaxine extended-release capsules, it is sensitive, specific (interference < 2%), precise (RSD < 2%), linear (r > 0.99), accurate (98.0 to 102.0%) and reproducible (RSD < 2%).Item Eletroanálise aplicada a ensaios de compatibilidade e estabilidade de fármacos(Universidade Federal de Goiás, 2020-08-31) Carvalho, Murilo Ferreira de; Garcia, Luane Ferreira; http://lattes.cnpq.br/6624146379323596; Gil, Eric de Souza; http://lattes.cnpq.br/3218622824233303; Garcia, Luane Ferreira; Leite, Karla Carneiro de Siqueira; Alecrim, Morgana Fernandes; Marreto, Ricardo Neves; Oliveira, Thiago Levi SilvaIntroduction: The pharmaceutical industry faces a major challenge to improve the solubility of some active ingredients. Carvedilol (CRV) is the drug of therapeutic choice in cardiovascular diseases. CRV presents low water solubility and low bioavaliability. Several researches were performed in order to increase the oral bioavailability, where selfemulsifying appeared as viable alternatives. Objective: The objective of this work was to evaluate interactions between CRV and its excipients, detecting the oxidative potential of these substances, thus, providing predictive data for the oxidation that may affect the stability and bioavailability of the drug system. Methodology: In the carbon pastes preparation were used 70 mg (graphite) and 30 mg (oil). Six excipients, oleic acid, canola, capmul, safflower, sesame and Plurol isoestearique. The following proportions were used in the making of the paste: 30:0, 20:10, 15:15, 10:20 and 0:30 of liquid excipient (sample): mineral oil (mg:mg) and 1.5:68.5, 3:67 and 7:63 of solid excipient (sample): graphite (mg:mg). CRV was incorporated in the oil moiety for solubilization and after used in solidstate analysis in the carbon paste. The techniques used were cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy. Results and Discussions: The Isostearique Plurol® was found to be electro-active in the studied potential, however, this oil oxidizes in different potentials from CRV. The results show that Isostearique Plurol®, liquid excipient, isoestearique acid and the solid excipient presented the greatest variations of anodic potential and were considered the best excipients for the CRV formulation. CRV presented higher stability at room temperature (25 ºC), whereas, it presented higher stability at 50 ºC when mixed with 7% estearique acid. Conclusions: Evidences of incompatibility were not found, relative to the increase of oxidation vulnerability, when in the presence of these excipients. The employment of electroanalysis for CRV compatibility studies show promising viability.Item Glicosilação regiosseletiva da hesperetina catalisada por Cunninghamella echinulata ATCC 9244, em biorreator(Universidade Federal de Goiás, 2019-03-19) Carvalho, Pedro Henrique de Oliveira; Oliveira, Valéria de; http://lattes.cnpq.br/6300240031300604; Oliveira, Valéria de; Arruda, Evilanna Lima; Bara, Maria Teresa Freitas; Santiago, Mariângela FontesIntroduction: Hesperetina is a flavonoid, belongs to flavanones class, with several pharmacological activities, the most cited being the antioxidant potential. However, it presents low solubility and consequently low bioavailability, making it difficult to apply it in a systemic pharmaceutical formulation. Glycosylation of hesperetin effectively modifies its pharmacokinetic properties, increasing its antidiabetic, antitilipidemic activity and efficacy against helicobacter pylori. Filamentous fungi are used in biotechnological processes to increase the activity of molecules, being able to glycosylate, regio and stereoselective derivatives, with less cytotoxicity. Cunninghamella belongs to the class of filamentous fungi capable of catalyzing glycosylation reactions. With the demand of scale-up the production of bioactive compounds, bioreactors are a promising strategy, providing a controlled environment. Aim: The objective of this work was to scale-up the regioselective glycosylation of hesperetin catalyzed by Cunninghamella echinulata 9244 in a bioreactor, with pharmacological activities improved. Methods: Hesperetin was incubated with the microorganism in PDSM medium on two scales, semi-preparative (100 mL) and preparative (2 L). HPLC-MS was used to monitoring and the caracterization of the product formed was confirmed by 1H13C NMR. Tests of anticancer and cytotoxicity were realized. Results and Discussion: The glycosylation yield in the semi-preparative scale was 0.8%, while in the preparative scale the result was 22.9%. The increase in yield can be explained by the automated control of the main parameters such as pH and oxygenation. The ideal time for biosynthesis of the glycosylated derivative in bioreactor was 72 hours. The purified product glycosylated was identified by NMR in hesperetin 7-O-β-D-glucopyranoside, not exhibiting anticancer activity, but with reduction of cytotoxicity, with IC 50 9.5 times lower than the starting compound. Conclusions: Regioselective glycosylation of hespertin was performed in a single step in bioreactor, significantly increasing the yield. The chromatographic procedure proposed by the HPLC-MS allowed a fast and efficient identification and characterization of the derivative.Item Estudo clínico de segurança não controlado do uso de drogas vegetais em pacientes atendidos em um ambulatório público de fitoterapia(Universidade Federal de Goiás, 2020-03-02) Cirilo, Hérica Núbia Cardoso; Cunha, Luiz Carlos da; http://lattes.cnpq.br/6349547031976679; Bara, Maria Teresa Freitas; http://lattes.cnpq.br/3914164125498267; Bara, Maria Teresa Freitas; Santos, Thalyta Renata Araújo; Alves, Suzana Ferreira; Paula, José Realino de; Soares, Amanda QueirozIntroduction: The use of medicinal plants has been increasing worldwide, so as the reports of adverse events (AE) related to them. The World Health Organization Toxicity Grading Scale for Determining the Severity of Adverse Events (WHO-TGS) establishes the toxicity criteria for laboratory tests in clinical trials with humans, allowing to evaluate the safety of a treatment. Additionally, the Naranjo Algorithm establishes the causal relationship between the observed AE and the product under investigation. Main objective: To verify the safety of the use of 12 medicinal plants in subjects attended at a phytotherapy outpatient clinic of the Unified Health System, using the WHO-TGS and the Naranjo Algorithm. Methodology: This is an open and prospective uncontrolled "before and after" clinical study. The selected participants were submitted to laboratory tests before and after 30 days of use of the prescribed medicinal plants. The results were analyzed according to WHO-TGS. The medicinal plants were suspended for 30 days in those who presented laboratory alterations indicative of toxicity, the exams were repeated, and the results analyzed. The observed AE had their causality established through the Naranjo Algorithm. Results and discussion: There was a higher prevalence of female individuals, mean age of 46 years, graduated level, who sought the health service to treat depression and anxiety. The majority of them are non-users of tobacco and illicit drugs, while half regularly consume alcoholic beverages, and are characterized mainly by the daily use of medicinal plants, plant drugs and herbal medicines for therapeutic purposes. 42 subjects were selected to use the medicinal plants prescribed for 30 days, of which 17 (40.5%) did not present AE, whereas 25 (59.5%) presented Grade 1 toxicity (GT1) AE, mainly the increase in amylase concentration (n=9). After the suspension of the medicinal plants use, 14 participants continued to present GT1 AE, mainly hypomagnesemia and hematuria. The Naranjo Algorithm established a possible causal relationship between hyperamylasemia; hypomagnesemia; hematuria; AST, ALT and GGT increase; hyperglycemia; hypocalcemia; hypercalcemia; thrombocytopenia; prothrombin time elevation; proteinuria and the medicinal plants used. Conclusions: The results obtained revealed the socio-demographic, clinical, life habits and pharmacotherapeutic profile of the participants. They demonstrated the occurrence of AE after the use of Bauhinia forficata, Curcuma longa, Cynara scolymus, Equisetum arvense, Erythrina mulungu, Matricaria chamomilla, Melissa officinalis, Passiflora edulis, Phyllanthus niruri e Zingiber officinale, evidencing a possible causal relationship to the use of these medicinal plants. Such relationship corresponds to a low level of probability of occurrence of an AE by the products under investigation, that is, it demonstrates safety in the use of these medicinal plants, in the experimental conditions employed.Item Biossorção de micropoluentes em fibra de sabugo de milho e detecção analítica usando fluorescência de raio x, espectroscopia Raman e análise quimiométrica(Universidade Federal de Goiás, 2021-04-07) Golveia, Jhéssica Cavalcante de Souza; Schimidt, Fernando; http://lattes.cnpq.br/1884442831412446; Santiago, Mariângela Fontes; http://lattes.cnpq.br/7143224488081563; Col, José Augusto da; Zang, Warde Antonieta da Fonseca; Morais, Mariana Cristina de; Sanches, Renato; Schimidt, FernandoThe presence of micro-pollutants in the waters has led to growing concern due to the impact on human health. Among the most important molecules is Bisphenol-A (BPA), pesticides, hormones and dyes. On the other hand, micro-pollutant removal processes that make use of agro-industrial waste have aroused the interest of researchers. This is because the accumulation of lignocellulosic materials is also an environmental problem. The adsorption capacity was tested using the molecules BPA, diuron, cyproterone acetate, caffeine, erythrosine red and tartrazine yellow. The detection of these pollutants requires the use of sensitive and easy-to-handle techniques. In this context, the X-ray Fluorescence (FRX) and Raman spectroscopic methods are highlighted.The objective of this work is to use corncob agroindustrial residue (CC) as an adsorbent in the removal of micropollutants of aqueous matrix and detect them using FRX and Raman Spectroscopy. The values was treated using Chemoface Software and PLS (Partial Least Square) Model of calibration. The adsorption studies were carried out in reaction media containing the ground vegetable fiber and different concentrations of molecules, as well as at different pHs. Isotherms models and kinetic models were evaluated. The results showed that CC is a potent adsorbent in aqueous solution, leading to about 90% of removal for BPA. The adsorption kinetics followed the pseudo-second order model and isotherm that best suited the Lagmuir model, and the maximum adsorption capacity was 51.25 mg per gram of adsorbent fiber, after 20 minutes of contact. The chemometric analyze showed hat PLS model allows to determine a calibration model capable to reach high values of R2 and low values of error. The thermogravimetric analysis allowed to conclude that it is a material resistant to high temperatures, due to the presence of considerable amount of lignin. Finally, the best results of BPA removal efficiency by CC compared to activated carbon corroborate its high biosorption capacity.Item Dispositivo de liberação intravítrea para tratamento de degeneração macular relacionada à idade(Universidade Federal de Goiás, 2023-04-04) Guerra, Maria Carolina Andrade; Fialho, Sílvia Ligório; http://lattes.cnpq.br/6277013725246341; Cunha Júnior, Armando da Silva; http://lattes.cnpq.br/8278273904187275; Cunha Júnior, Armando da Silva; Lima, Eliana Martins; Dias, Marina França; Dourado, Lays Fernanda Nunes; Fialho, Silvia LigórioAge-related macular degeneration, AMD, is a chronic eye disease which damages the macular area. It is the main cause of adult blindness worldwide. The pharmacological treatment of choice for the neovascular form of AMD consists of intravitreal injections of anti-VEGF agents that, occasionally, might be combined with corticosteroids. Intravitreal injections are the preferred route for drug delivery to the posterior segment of the eye but have limited therapeutic success due to the quick elimination of substances, requiring frequent injections that bring complications to the patient and low adherence to treatment. Intravitreal implants are capable to maintain therapeutic levels of drugs for a long period. Biodegradable nanofibers can modulate drug release in addition to allowing the incorporation of fragile bioactive substances. The present work aimed the development of biodegradable intravitreal implants containing dexamethasone coated with polymeric nanofibers containing bevacizumab as a therapeutic proposal for the treatment of AMD. Nanocoated implants were successfully developed. The characterization of the system, the study of degradation and efficiency of the coating process were evaluated by scanning electron microscopy. The drug release profile was determined by high performance liquid cromatography and the Bradford method. The antiangiogenic activity was determined by the chicken embryo chorioallantoic membrane model. The safety of the formulation was evaluated in rabbit eyes. So far, the system was successfully developed. The study degradation study confirmed the presence of nanofibers on the implant surface for up to 35 days. The degradation study demonstrated the presence of nanofibers on the surface of the implant for up to 35 days. The release profile showed that 68% of dexamethasone was released within 35 days and 90% of bevacizumab within 48 hours. The formulation presented activity in reducing vessels and was safe for the rabbits’ retina. No clinical or histopathological changes were observed, neither alteration in retinal thickness by optical coherence tomography during the 28 days. The developed implants, therefore, may be considered as a new alternative for the treatment of AMD.Item Modelos de aprendizado de máquina para avaliação preditiva de toxicidade dérmica aguda de compostos químicos(Universidade Federal de Goiás, 2020-12-16) Hall, Steven Umualê Silva; Alves, Vinicius de Medeiros; http://lattes.cnpq.br/7314022014345242; Andrade, Carolina Horta; http://lattes.cnpq.br/2018317447324228; Andrade, Carolina Horta; Scotti, Marcus Tullius; Neves, Bruno JuniorIntroduction: Acute dermal toxicity is a collection of adverse effects that a substance can cause in the first 24 hours of dermal exposure to this chemical. This toxicological property using animals by estimating the lethal dose (LD50), the dose required to kill 50% of individuals of a test population. However, due to public and political pressure on issues related to animal testing, alternative methods are becoming essential to reduce the costs and number of test animals. Computational methods such as machine learning methods have been presented as a reliable alternative to animal testing for hazard assessment. Objectives: The main goal of this study was to develop machine learning models capable of predicting acute dermal toxicity of chemicals, and to make these models available in an online server for the scientific community. Methodology: Acute dermal toxicity datasets where compiled from literature and rigorously curated. Classificatory and multi-classificatory QSPR (Quantitative Structure-property Relationships) models were developed using molecular descriptors and machine learning algorithms and where then used to screen the CosIng library as a case study. Results and discussion: After data curation, 2,622 compounds were kept in the dataset (384 toxic and 2238 non-toxic). To avoid developing biased models, the dataset was balanced, and 768 (384 toxic and 384 non-toxic) were kept for the modeling. The best classificatory models were generated with random forest algorithm and achieved CCR of 79%, SE of 80%, SP of 78%. The best models were used in an integrated hierarchical strategy that obtained ACC= 74% and Recall =74%. In total 33 compounds from CosIng were correctly predicted as toxic. Conclusions: The developed QSPR models were robust and predictive and are capable of predicting acute dermal toxicity efficiently. These are thde first models developed for this endpoint which passed by a rigorous data curation and validation process, being valuable in the prediction of toxicity from new untested compounds. These models are available in a web server through the address https://stoptox.mml.unc.edu .Item Preparação e uso de eletrodos de frafite nanoestruturados de vanadato de bismuto na degradação fotoeletrocatalítica de radamina B isotiacianato(Universidade Federal de Goiás, 2023-02-10) Isecke, Bruna Guimarães; Teixeira, Guilhermina Ferreira; http://lattes.cnpq.br/5992362464264556; Gil, Eric de Souza; http://lattes.cnpq.br/3218622824233303; Gil, Eric de Souza; Oliveira, Sérgio Botelho de; Colmati Júnior, Flávio; Oliveira, Mayk Teles de; Prado, Lara Barroso BritoPhotoelectrocatalysis is a promising way to treat water contaminated by negative organic compounds. The use of BiVO4 nanoparticles supported on a conductive substrate allows the degradation of pollutants to be carried out under less energetic conditions. In this work, we report the degradation of Rhodamine B Isothiocyanto dye in a BiVO4/graphite (BVO@C) electrode performed under visible light irradiation through a photoelectrocatalytic process. The BiVO4 particles passed through ultrasonic irradiation by coprecipitation and then deposited on the surface of a graphite by the impregnation method. The 23 factorial design was used to optimize electrode fabrication. The electrode was characterized using SEM/EDS, XRD and DLS techniques. For the electrochemical characterization of the electrode, cyclic voltammetry (VC) and electronic impedance spectroscopy (EIS) were performed. The evaluation of the degradation of RhB isothionate was carried out in the processes of electrooxidation and photoelectrocatalysis, controlling or not controlling the temperature using the unmodified electrode and the BVO@C electrode. The characterization of the electrode confirmed the impregnation of the graphite with a BiVO4 particle through SEM/EDS, revealing specific peaks referring to vanadium (V), bismuth (Bi) and oxygen (O). The XRD analysis bought peaks related to the monoclinic phase of BiVO4, this fact being important due to the smaller band gap value, which implies activation of the semiconductor through light in the visible range. DLS and zeta potential analysis revealed negative charges on the surface of BVO@C, PDI values that indicated that the solution used for impregnation had monodispersive characteristics, which corroborated to a less regular impregnation, since this factor does not contribute to a deposition independent. Electrochemical analyzes confirm a considerable improvement of the modified electrode through a decrease in resistivity that may be associated with the presence of BiVO4, in addition to a decrease in hysteresis in relation to the emission/reduction pair. After 30 minutes, the degradation results in the electrocatalysis (EC) and photoelectrocatalysis (PEC) system, the results were considered relevant, reaching almost 100% of the dye being degraded, with a higher degradation rate for the photoelectrocatalysis experiments in relation to electroxidation, and higher values in experiments in which temperature was not controlled. To analyze the electrode reuse cycles, several reuse cycles were performed and the electrode efficiency (BVO@C) was observed. After nine cycles of reuse of the BVO@C electrode, it was observed that it presented more than 80% of RhB efficiency. and photoelectrocatalysis of dyes from industrial effluents.Item Avaliação do potencial antioxidante e atividade vasorrelaxante de Cinnamomum sp.(Universidade Federal de Goiás, 2023-05-16) Moreno, Emily Kussmaul Gonçalves; Macêdo, Isaac Yves Lopes de; http://lattes.cnpq.br/7801802836007488; Gil, Eric de Souza; http://lattes.cnpq.br/3218622824233303; Macêdo, Isaac Yves Lopes de; Garcia, Telma Alves; Rocha, Matheus Lavorenti; Carvalho, Murilo Ferreira de; Machado, Fabio BahlsThe great interest in the study of antioxidants is mainly the prevention of physiological damage caused by oxidative processes. Cinnamon (Cinnamomum sp.), is a spice used worldwide in cooking and has gained prominence for its pharmacological properties, such as anti-inflamatory, antibacterial, antifungal, cardiovascular, among others. Given the importance of determining the antioxidant capacity in relation to therapeutic methods, this study aims to evaluate the antioxidant profile of commercial cinnamon samples by spectrophotometric, electrochemical and vasorelaxant methods. The spectrophotometric methods performed were 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azinobis (3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) and Folin-Ciocalteu. For the electrochemical experiments, a three-electrode system was used, consisting of carbon paste electrode, platinum wire, and Ag/AgCl/KClsat, representing the working, auxiliary, and reference electrodes, respectively. The electroanalytical methods used were differential pulse, square wave, and cyclic voltammetries. Vascular reactivity was evaluated in aortic artery rings from male Wistar rats, using arteries with intact vascular endothelium and without endothelium. The endothelial nitric oxide synthase (eNOS) pathway and the inhibitor N-nitro-L-arginine-methylester (L-NAME) were used in this analysis. The correlation between the electrochemical approach and total phenols by the ABTS, DPPH, and Folin- Ciocalceu methods were 0.63, 0.70, and 0.73, respectively, with 1 being an ideal correlation. The correlation between spectrophotometric methods was 0.83. For the electrochemical results, a similar profile was observed, with anodic peak a.c Epa1= 0.1 V, indicative of polyphenolic compounds with high antioxidant power. In addition, a biosensor on a carbon paste electrode was applied to the selected extracts using laccase enzyme, obtained by the fungus Marasmiellus sp. and a signal improvement of up to 4 times in the antioxidant profile was observed when compared to the electrode without modification. The samples were analyzed by mass spectrometer, and the main chemical markers found were coumarin, cinnamaldehyde, and eugenol. Pharmacological assays showed that these samples also promoted a significant vasorelaxant effect associated to the antioxidant potential. Thus, cinnamon showed a high antioxidant capacity, in agreement with the results obtained in other studies, emphasizing its importance as a functional food.Item Desenvolvimento de nanopartículas lipídicas e associação de iontoforese para administração transdérmica do lopinavir(Universidade Federal de Goiás, 2020-03-30) Moura, Rayssa Barbary Pedroza; Andrade, Lígia Marquez; http://lattes.cnpq.br/9862318690104720; Taveira, Stephânia Fleury; http://lattes.cnpq.br/0382450621383005; Taveira, Stephânia Fleury; Marreto, Ricardo Neves; Gelfuso, Guilherme MartinsIntroduction: Lopinavir (LPV) is one of the antiretrovirals used to combat the human immunodeficiency virus (HIV). It has low oral bioavailability, a complex therapeutic regimen, and adverse effects, which affect the efficiency of the therapy. Transdermal administration can circumvent or reduce these problems. However, it requires strategies that facilitate the permeation of the drug. It is believed that nanostructured lipid carriers (NLC) combined with iontophoresis can increase the permeation of LPV to the deeper layers of the skin to be absorbed systemically. Objective: Develop NLC containing LPV and evaluate the permeation of the drug with and without iontophoresis. Methodology: The analytical method was adapted and validated. The solubility of the drug in surfactants was evaluated to select those that most solubilize LPV. NLC with different concentrations of LPV (0.5 - 1.5 mg/mL) were produced. Full characterization was performed (mean diameter, polydispersity index (PdI), zeta potential, encapsulation efficiency (EE), recovery (REC), drug loading (DL), and morphology). Electrical stability studies of the drug and NLC were carried out, as well as studies of electronic paramagnetic resonance (EPR), release and permeation in vitro, with and without electric current. Results and discussion: The method was linear, precise, and accurate. The NLC was spherical (145.83 ± 5.229 to 179 ± 2.551 nm). The PdI and zeta potential indicated good homogeneity and stability of the NLC. EE was around 80%, and, therefore, particles with 1.5 mg/mL of LPV were selected for subsequent studies. The stability studies of NLC-LPV concerning electric current demonstrated that the application of iontophoresis significantly reduced EE. The EPR spectra showed a significant increase in the nanoparticles' stiffness when an electrical current was applied. The electrical current also significantly increased the drug release in 6 h study compared to studies without electrical current. These data demonstrate that the electric current is a trigger in the drug release since it increases the rigidity of the lipid matrix. Iontophoresis also significantly increased the permeation of LPV. With an electrical current, LPV quantification was 14,49 ± 3,98 and 21,85 ± 2,28 μg after cathodic and anodic iontophoresis. These permeation data are significantly higher than the drug's EC50 values for various strains of the virus, reported in the literature. Conclusion: The combination of iontophoresis with NLC enabled higher permeation of LPV and proved to be a promising strategy for transdermal drug administration. However, in vivo, and pharmacokinetic studies are needed to assess the effectiveness of the strategy used in this study.Item Produção de lacases por Pleurotus ostreatus e o uso da biorremediação e adsorção para tratamento de poluentes via biomassa residual(Universidade Federal de Goiás, 2022-02-24) Nunes, Elaine Sousa; Santiago, Mariângela Fontes; http://lattes.cnpq.br/7143224488081563; Santiago, Mariângela Fontes; Paula, José Realino de; Morais, Mariana Cristina de; Zang, Warde Antonieta da Fonseca; Faria, Fabrícia Paula deThe contaminants of effluents with domestic and industrial releases have been proven in several regions of the planet. In the face of outbreaks caused by flaviviruses the use of repellents has grown significantly, as well as synthetic dyes, because they are widely used in the textile industry and bisphenol A, because it is found in different commercial polymers, are commonly found in pollutants keeping present in waters generating possible harmful effects. Conventional effluent treatment processes have low chemical removal efficiency and are expensive. In view of this, the objective of this study was the development and evaluation of methodologies for the treatment of effluents contaminated with the active ingredient of repellents, icairidina, red textile dye Drimaren CL-5B and bisphenol A (BPA) by using açaí residue as adsorbent material and/or bioremediation with the lacase produced from the white decomposition fungus Pleurotus ostreatus using as enzymatic inducer the solid residues of acai, pineapple, mexerica and pequi. The enzymatic production with the residues of açaí and pineapple obtained an activity of 1139 UmL-1 and 1031 UmL-1 respectively. Remediation was efficient for BPA, being able to bioremediate up to 100 % of the compound, but it was ineffective for icariride. However, açaí revealed high adsorptive capacity, reaching to fully adsorb bisfinel A, more than 50 % of iridin and more than 70 % of the dye when in a synthetic sample and 40 % in an industrial sample, demonstrating a promising methodology.