Doutorado em Genética e Melhoramento de Plantas (EA)
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Navegando Doutorado em Genética e Melhoramento de Plantas (EA) por Por Orientador "Soares, Thannya Nascimento"
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Item Variabilidade genética de Dipteryx alata Vogel (Leguminosae): novos marcadores microssatélites, fluxo de pólen in situ e ex situ e genética da paisagem em escala local(Universidade Federal de Goiás, 2018-02-23) Guimarães, Rejane Araújo; Telles, Mariana Pires de Campos; http://lattes.cnpq.br/4648436798023532; Soares, Thannya Nascimento; http://lattes.cnpq.br/5590256762396056; Soares, Thannya Nascimento; Mansano, Vidal de Freitas; Borba, Tereza Cristina de Oliveira; Chaves, Lázaro José; Diniz Filho, José Alexandre FelizolaDipteryx alata is a Neotropical tree widely distributed throughout the Brazilian Cerrado. Popularly known as baru, it is one of the most promising Cerrado species for domestication and cultivation due to its wide potential use. The general goal of this work was to assess reproductive system and pollen-mediated gene flow patterns of in situ and ex situ conditions and to evaluate how landscape may influence intrapopulation genetic structure. New microsatellite markers were developed using high performance sequencing technologies, then the ten most polymorphic were selected and used in this work. The evaluation of the reproductive system and pollen dispersal was carried out in a germplasm collection (ex situ) located at the Universidade Federal de Goiás and in one natural population (in situ) in Orizona-GO, central Brazil. The natural population was also used to evaluate landscape influence on intrapopulation genetic structure in adult and juvenile trees. In situ and ex situ conditions showed a high cross fertilization rate (tm = 0.815 and tm = 0.934), respectively, confirming that the D. alata presents a mixed-mating system, predominantly allogamous. The number of seeds sharing the same pollen donors was high, indicating a low number of pollen donors per tree under both conditions. This may be related to the proximity between groups of trees, considering that there is less cross-pollination between groups of more distant trees. Another factor that may have influenced cross-pollination over long distances is the heterogeneous landscape due to habitat fragmentation. The results indicate the presence of a positive and significant spatial genetic structure (SGS) in both stages of life (adults and juveniles) with the highest Sp value in juveniles. Bayesian cluster analysis showed the formation of two groups in both adults and juveniles. Juvenile individuals presented greater interference from landscape compositions. Thus, the inclusion of the landscape features brought an explanation gain to the pattern of intrapopulation genetic structure in the D. alata species. These results are important to support more efficient conservation strategies for this species.Item Filogeografia de Pterodon emarginatus e Pterodon pubescens (Leguminosae)(Universidade Federal de Goiás, 2019-12-06) Lima, Stefany Lorrayny; Soares, Thannya Nascimento; http://lattes.cnpq.br/5590256762396056; Soares, Thannya Nascimento; Telles, Mariana Pires De Campos; Rocha, Dulce Maria Sucena da; Mansano, Vidal de Freitas; Jardim, Tatianne Piza Ferrari AbreuThe species Pterodon emarginatus and P. pubescens, known as sucupira, occur in the Cerrado and have potential for medicinal and wood use. The comparative phylogeography of these species can help in understanding the historical pattern of distribution of genetic diversity, generating information for use and conservation. The objective of this work was to identify the phylogeographic structure of P. emarginatus and P. pubescens, and the comparative phylogeography of these species. The sample consisted of eleven populations for P. emarginatus and nineteen for P. pubescens, distributed in the states of Goiás, Tocantins, Bahia, Mato Grosso and Minas Gerais. The chloroplast regions sequenced were trnLE-trnLF and psbA-trnH, and the nuclear ribosomal was ITS101-102. In P. emarginatus, haplotypic and nucleotide diversity are high in chloroplast, as there is a high polymorphism in this region, with a high number of indels. The population structure is relatively low (FST = 0.10). In this species the populations Alvorada do Norte-GO, Cariri-TO, Luiz Eduardo Magalhães-BA and Monte Alegre de Goiás-GO stand out for genetic diversity and are located in an area of climate stability, influencing the evolutionary dynamics of the Cerrado. The dispersal has long distance potential (500 km). The demographic pattern has shown population growth for less than 150 a thousand years. In P. pubescens the haplotypic and nucleotide diversity are high in chloroplast and nucleus, with a high number of haplotypes. The population genetic structure is considerable (FST = 0.24), with a phylogeographic pattern marked by the divergence of populations of the Araguaia basin region, an area of influence on the Cerrado evolutionary dynamics, mainly Araguapaz-GO and Araguaiana-MT. Demographic history has shown population growth from 1.67 million years ago. Finally, P. emarginatus showed a higher diversity pattern in chloroplast, while in P. pubescens there was a high complexity of the phylogeographic pattern, and the population of Araguaiana-MT showed a high genetic distance. Despite the species separation, there is a highlight for populations that exhibit high haplotype sharing, mainly Araguaiana-MT and Araguapaz-GO. The species tree showed no distinction between species, corroborating the hypothesis of recent diversification. Comparative analysis of species suggests ancestral introgression or polymorphism, considering the high sharing of haplotypes. These species are possibly in the process of recent separation, and P. emarginatus is the species in the process of speciation.