Programa de Pós-graduação em Biologia da Relação Parasito-Hospedeiro
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Navegando Programa de Pós-graduação em Biologia da Relação Parasito-Hospedeiro por Por Orientador "Fiaccadori, Fabíola Souza"
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Item Investigação molecular de vírus em crianças com infecção do trato respiratório e crianças asmáticas em Goiânia-Goiás(Universidade Federal de Goiás, 2015-04-23) Castro, Ítalo de Araújo; Fiaccadori, Fabíola Souza; http://lattes.cnpq.br/0993842409303174; Fiaccadori, Fabíola Souza; Souza, Menina Borges de Lima Dias e; Costa, Paulo Sérgio Sucasas daAcute respiratory infection (ARI) is a major cause of morbity and mortality worldwide, particularly among children, and most of these infections are caused by viruses. Respiratory viral infections can cause symptoms ranging cough, coryza, sneezing, fever and airflow obstruction. Furthermore, the infection poses as an important trigger of asthma exacerbation, frequent clinical condition in children, and its prevalence has been rising in the last years. There are few epidemiologic studies analyzing the relationship between ARIs and asthma in Brazil. Based on this background, the aim of the study was investigate the occurrence of viral respiratory infections in pediatric patients with and without asthma in Goiânia – Goiás. Between august, 2012 and august, 2013 225 nasal aspirates and/or nasal swab samples were obtained from children with four to 14 years old. The samples were screened by Multiplex Nested-PCR for detection of 16 common respiratory viruses. From 225 samples, 42 had at least one virus detected. Samples from four different patients had more than one virus detected. The viral detection rate in ARI patients (25%), exacerbated asthma (16.3%) and stable asthma (14.8%) showed no significant difference. The most frequent viruses detected were Rhinovirus (28.6%), FLUA (11.9%), Adenovirus (11.9%), HBoV (11.9%) and RSVA (9.5%). The monthly detection rate was higher during the rainy season, period marked by great rainfall and high relative air humidity. Among the positive samples, RSV were detected during the great rainfall months and high air humidity, while the FLU and HBoV were detected during the winter months, period with low air humidity in the Mid-West region. The seasonal profile from the other viruses was unclear. The obtained results reinforces the importance of the viral pathogens in the pediatric population. Although the viral detection rate was not statistically significant, the presence of these pathogens in children is an important matter for consideration, especially to delineate control and prevention measures concerning ARIs and its impact on preexistent asthma. Hence, this study is the first of the kind in the region, and the data provided tried to fill the knowledge gaps about seasonality and circulation of these pathogens.Item Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás(Universidade Federal de Goiás, 2015-04-17) Cunha, Marielton dos Passos; Fiaccadori, Fabíola Souza; http://lattes.cnpq.br/0993842409303174; Fiaccadori, Fabíola Souza; Coelho, Alexandre Siqueira Guedes; Cardoso, Divina das Dôres de PaulaDengue viruses (DENV) serotypes 1, 2, 3, and 4 have been causing yearly outbreaks in Brazil. Nevertheless, the population structure of the viruses transmitted in Goiás state is not well understood. In this study, we investigated the phylogenetic pattern of DENV samples identified in Goiânia, Goiás, Brazil during the 2012/2013 epidemic. Therefore, the entire region of the gene of the envelope (E) protein (1485bp) of 16 DENV-1 samples as well as partial region of this gene (363bp) of seven DENV-4 samples were sequenced. Phylogenetic analysis showed that DENV-1 belongs to the genotype V and presents itself divided into two clades, suggesting co-circulation of two distinct lineages in this region. Still, the molecular analyzes indicated a significant change in the amino acid level E348 position. For DENV-4, the sequences were segregate in a monophyletic group and are classified as genotype II American subclade. The molecular and phylogenetic analysis showed that the region suffered multiple introductions by dengue virus. This is the first report of co-circulation of two lineages of DENV-1, and the circulation of DENV-4 in Goiás state.Item Identificação e caracterização molecular do vírus dengue em indivíduos sintomáticos atendidos na rede pública de saúde de Goiânia – Goiás, durante o período epidêmico 2012-2013(Universidade Federal de Goiás, 2014-07-18) Guimarães, Vanessa Neiva; Fiaccadori, Fabíola Souza; http://lattes.cnpq.br/0993842409303174; Souza, Menira Borges de Lima Dias e; Cardoso, Divina das Dores de Paula; Fiaccadori, Fabíola SouzaDengue is a major challenge to public health in Brazil and worldwide. Dengue virus (DENV) is an arbovirus of the family Flaviviridae, genus Flavivirus, classified into four antigenically distinct serotypes DENV1-4. Since the introduction of DENV1 in Goiás, the current situation consisted of successive epidemics of dengue fever with change among prevalence serotypes. One of the factors that have been associated with the pathogenesis of this infection is the occurrence of a cross-serotype immune response in secondary infections. Thus, the identification of circulating serotypes and also the characterization of the genomic variants have been considered important on disease surveillance, since the introduction of new variants can be a risk factor for severe dengue. In this context, the present study aimed to investigate the occurrence of DENV infection in individuals treated at health centers in Goiânia, Goiás in the epidemic period 2012-2013, using serological and molecular methods for determining the rate of positivity and identification of serotypes/genotypes in the circulating virus. For this, 278 samples from suspected individuals of DENV infection, which were submitted to the research of serological markers NS1, IgM and IgG using a commercial kit, as well as viral RNA detection and serotype identification by RT-PCR for region C-prM. The positivity for infection was 43.9%, with rates of 30.5%, 13.6% and 20.5% for NS1, IgM and RNA, respectively. Considering the RNA-positive samples, 31.5% were DENV1 and 68,5% were DENV4, which were subjected to nucleotide sequencing of the E/NS1 junction to characterize the viral genotype. Phylogenetic analysis classified the DENV1 samples as genotype V, that differentiated into two clades, which may reflect the occurrence of genetic variants with differences in evolutionary history. For DENV4 samples, classified as genotype II, was observed a phylogenetic proximity between the strains analyzed and others strains of the country and Latin America. These results demonstrate the effectiveness of the combination methods in the diagnosis of DENV infection. Still, the molecular evaluation has proven crucial to establish a complete data base to be used for surveillance studies, collaborating with investigations of the dynamics of the virus and the expected profile for future epidemics.Item Avaliação da exposição ao vírus chikungunya em população de Goiânia-Goiás(Universidade Federal de Goiás, 2019-03-29) Morais, Paulo Henrique de; Fiaccadori, Fabíola Souza; http://lattes.cnpq.br/0993842409303174; Fiaccadori, Fabíola Souza; Souza, Menira Borges de Lima Dias e; Féres, Valéria Christina de RezendeThe Central-West region presents wide circulation of different arboviruses, such as Zika virus (ZIKV), Dengue virus (DENV) and Chikungunya virus (CHIKV). Chikungunya fever is a disease of sudden onset, transmitted by mosquito vectors, intermediate hosts of CHIKV. This pathology represents a significant health problem for the infected person, and the disease can cause symptoms that can be prolonged for more than two years, such as polyarthralgia and myalgias, requiring greater recovery care than other arboviruses. The present study aimed to investigate the seroepidemiological profile of chikungunya virus infection in the population of the city of Goiânia-GO, in the years 2016 and 2018. The study population consisted of 174 individuals attended by the public health network of the municipality, with a clinical picture suspected of CHIKV infection in the years 2016 and 2018. The collected serum samples were submitted to the enzyme-linked immunosorbent assay for the detection of specific antibodies against CHIKV class G (anti-CHIKV-IgG), using commercial kit. A global positivity index for CHIKV of 2.3% was identified. Of the individuals investigated, four cases of IgG marker positivity were identified, two male and two female, being described in both the symptomatic picture of fever, myalgia and multiple arthralgias. Studies evaluating seroprevalence for CHIKV infection are still scarce in the country and the data obtained in the present study demonstrate the need for continuous monitoring and investigation of the circulation of this agent in the region.Item Investigação molecular de vírus respiratórios em população pediátrica em Goiânia, Goiás(Universidade Federal de Goiás, 2016-04-08) Oliveira, Anniely Carvalho Rebouças; Fiaccadori, Fabíola Souza; http://lattes.cnpq.br/0993842409303174; Fiaccadori, Fabíola Souza; Souza, Menira Borges de Lima Dias e; Martins, Regina Maria BringelThe acute respiratory infections (ARIs) are an important cause of morbidity and mortality worldwide. Viruses have an important role in the etiology of the respiratory infections. Children less than five years of age have about four to six ARIs per year and this is a common cause of hospitalization, mainly in developing countries. In Brazil, particularly in the West-Central region, studies that evaluated the circulation of respiratory viruses in the pediatric population are scarce. Thus, this study aimed to investigate the occurrence of respiratory viruses in the pediatric population, from Goiânia-Goiás. Between May/2014 and May/2015, samples of nasal swabs were collected from children from zero to six years of age presenting or not respiratory symptoms, attended at the Materno Infantil hospital in Goiânia. For the molecular screening, 16 viruses were investiged using three Multiplex Nested-PCR protocols. It two hundred and fifty one samples were collected, of wich 90 (35,9%) were positive for at least one respiratory agent, being rhinovirus (31%), respiratory syncytial virus (27,4%) and parainfluenza virus (13,3%) the most prevalent. Children under two years of age had greater overall positive, although not statistically significant. Similar detection rate was observed among the groups symptomatic (37%) and asymptomatic (34,5%). For the symptomatic group, children whose samples were collected during the dry period (p<0,05) and those with up to two years of age were most affected (p<0,05). In the asymptomatic group no statistical difference was observed. It was observed rate of co-detection of 6,4% (16/251), mostly in samples of symptomatic patients (13/16) (p<0,05). The evaluation of the temporal profile showed a higher detection rate during the dry season, less rainfall, relative humidity and cooler temperatures. The results reinforce the importance of respiratory viruses in children and contribute to further understanding of the epidemiological and temporal factors associated with different pathogens in our region. Thus, they open the way to new studies in the state of Goiás, also providing information to assist in the construction of control measures and more effective prevention of these infections.Item Investigação de Zika vírus em gestantes exantemáticas: identificação de positividade viral prolongada(Universidade Federal de Goiás, 2018-10-25) Oliveira, Thais Santana de; Fiaccadori, Fabíola Souza; http://lattes.cnpq.br/0993842409303174; Fiaccador, Fabíola Souza; Souza, Menira Borges de Lima Dias e; Silva, Marcelle Figueira Marques daZika virus (ZIKV) has recently emerged causing epidemics that impacted in the Americas, Africa and Asia and, despite transmission by arthropod vectors, sexual and maternal-child transmission have already been reported. Infection during pregnancy has been associated with congenital malformations, including microcephaly. Although we have advanced in ZIKV study, there are still gaps regarding the pathogenesis of this infection. The present study objected to investigate the occurrence of ZIKV in cases of exanthematic pregnant women evaluating prolonged viral detection in urine and blood samples. The population consisted of 68 pregnant women, of whom 57 were followed up through periodic consultations, and blood and/or urine samples were collected at each visit. Samples were subjected to detection of viral RNA by real-time RT-PCR using the commercial kit, primers and probe specific for the target region to structural protein E. Positivity rate of 51.5% for ZIKV was observed (35/68). The samples colected of follow up patients, different patterns of prolonged viral detection in the blood and/or urine were identified in 19.3% of pregnant women. In urine samples, viral RNA was detected up to 147 days after onset of symptoms and in the blood up to 187 days after the onset of rash. Among the pregnant women with prolonged detection profile, the majority presented the exanthematic episode at the first and second gestational trimester. Prolonged detection for ZIKV has been reported in other clinical specimens although many questions remain. In this scenario, the information obtained in the present study contributes to the knowledge about the process of the pathogenesis of Zika virus infection in infected pregnant women.Item Investigação da ocorrência do vírus chikungunya em gestantes com quadro exantemático em Goiânia-Goiás(Universidade Federal de Goiás, 2018-11-30) Silva, Lucélia Barbosa de Queiróz; Féres, Valéria Christina de Rezende; http://lattes.cnpq.br/8089054699896454; Fiaccadori, Fabíola Souza; http://lattes.cnpq.br/0993842409303174; Fiaccadori, Fabíola Souza; Carneiro, Megmar Aparecida dos Santos; Fonseca, Simone Gonçalves daThe chikungunya vírus (CHIKV) is an alphavirus transmitted by mosquitoes of the genus Aedes associated with an infection characterized by fever, myalgia, rash and persistent arthralgia. In 2014 with the arrival of the zika virus in Brazil and its association with vertical transmission and consequent microcephaly in newborns, many questions were postulated regarding the transmission of other arboviruses. In this context, several studies point to the perinatal transmission of CHIKV with encephalitis in neonates. In view of these facts, the present study aimed to investigate the occurrence of chikungunya virus in exanthematic pregnant women, in the city of Goiânia, from January 2017 to July 2018. The study population consisted of 70 pregnant women, from which samples were collected which were subjected to the screening of IgM / IgG serological markers using commercial kit, as well as the detection of viral RNA through RT-qPCR. A CHIKV seroprevalence index of 2.8% was identified. Of the pregnant women investigated, a case of positive IgG marker was identified (Case 1) and another showed positive positivity (IgM-IgG-RNA) (Case 2), being described in both cases the symptomatic picture in the first gestational trimester. In these cases, the newborns were positive only for the IgG marker. This is the first study to investigate the occurrence of CHIKV in the state of Goiás, showing a low circulation of CHIKV in our region, especially in the pregnant population. The information obtained reinforces the importance of studies in this population, in order to better understand the profile of this infection in pregnant women, as well as to contribute to the understanding of aspects associated with the pathogeny of this agent.Item Investigação do Metapneumovírus em população pediátrica atendia em um hospital da rede pública de Goiânia - Goiás(Universidade Federal de Goiás, 2017-11-13) Sousa, João Paulo Gomes de; Fiaccadori, Fabíola Souza; http://lattes.cnpq.br/0993842409303174; Fiaccadori, Fabíola Souza; Costa, Lusmaia Damaceno Camargo; Souza, Menira Borges de Lima Dias e; Carneiro, Megmar Aparecida dos Santos; Matos, Marcia Alves Dias deThe human metapneumovirus, first discovered and described in 2001, has been related to infections in the upper and lower respiratory tract, in all age groups, with severe cases frequently associated with children under five years old, elderlies and immune-compromised people. The present study aims to investigate the occurrence of hMPV in the pediatric population attended in the pediatry referral hospital, in the city of Goiânia - Goiás, evaluating symptomatic and asymptomatic patients. During the period of may/2014 and may/2015 251 nasal swab samples were collected from kids with age up to six years old, with respiratory infection symptoms or asymptomatic. The viral RNA was extracted using the QIAamp cador pathogen mini kit, and the extraction products were submitted to the reverse transcription reaction. The cDNA was submited a nested-PCR using specific primers to the amplification of a conserved region corresponded to the M gene. It was observed a global positivity index for the hMPV of 14.7% (37/251). In the group of children with respiratory infection, the positivity index was 16.6%, however, a significant index was also observed among asymptomatic children (12.3%). Analysis regarding the sample collection period did not show a statistically significant difference, reinforcing literature data that describe the circulation of this pathogen all year long. In Brazil there are few studies regarding the epidemiology of hMPV infections, being this the first report of hMPV occurrence in midwest, contributing to a better understanding of the role played by hMPV in the context of respiratory tract infections.