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Item Tipagem molecular, detecção de genes de virulência e determinação do potencial patogênico de isolados de Escherichia coli de patos (Cairina moschata), perus (Meleagris gallopavo), galinhas (Gallus gallus)(Universidade Federal de Goiás, 2019-02-28) Almeida, Ana Maria de Souza; Rezende, Cintia Silva Minafra e; http://lattes.cnpq.br/5841210447886226; Jayme, Valéria de Sá; http://lattes.cnpq.br/0603234425928309; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Moraes, Dunya Mara Cardoso; Cervi, Renato Clini; Nunes, Iolanda Aparecida; Porto, Regiane Nascimento GagnoThe general objective of this work is to evaluate the phylogenetics and to detect virulence genes from Escherichia coli isolates from different bird species, as well as to investigate their pathogenic potential in fertile broiler eggs. In the present study three experiments were carried out to analyze, in different aspects, E. coli isolates from different bird species. In experiment 1, E. coli isolated from ducks and turkeys were inoculated into broiler chicks one day to evaluate the pathogenicity of the strains. Fifty isolates of E. coli originating from ducks and 50 isolates from turkeys were tested for the genes iss, iucD, papC and tsh, all commonly found in APEC. Of the 100 isolates of E.coli analyzed, 42% (42/100) detected one or more genes and only 14.2% (6/42) had the four genes studied. After PCR, the positive strains were selected for the four genes common to APEC. Three isolates from ducks and three turkeys were purified separately and six inoculants were inoculated at 107 CFU / mL and inoculated via air sac in one - day - old chicks to evaluate the pathogenicity of these six positive isolates for the four genes. The chicks were evaluated twice a day for ten days in relation to mortality rate, clinical signs and macroscopic findings. The challenged chicks presented respiratory signs (sneezing, nasal discharge and rales) and macroscopic lesions such as aerossaculitis, persistence of the yolk sac, pericarditis and exudate in the nasal sinuses. In view of the results, it is concluded that E.coli originating from both ducks and turkeys are potentially pathogenic for day-old challenged cuttlefish chicks. In the experiment 2, embryos of broiler chickens were challenged with E. coli isolates positive for the iss, tsh, papC and iucD genes from hen, broiler, duck and turkey to analyze the pathological and hematological conditions , besides the performance of broiler chickens kept up to 42 days of age. Embryos with 18 days of incubation were challenged, via air chamber, with 0.1 mL of four different inoculants, according to the treatment, (T2 - E.coli of hen chicken, T3 - E.coli of broiler, T4 - E.coli of duck and T5 - E. coli of turkey) with concentration of 107 CFU / mL and with sterile saline solution (T1 - control group). Embryonic and post - hatch mortality rates, clinical signs, hematological alterations, interference of zootechnical performance and macroscopic findings during the incubation period and rearing up to 42 days of life were evaluated. At 14, 28 and 42 days, one bird from each of the four replicates of the five treatments. Regardless of the species of origin, E.coli isolates were not able to cause significant mortality in embryos challenged at 18 days of incubation. At birth and up to 42 days of age, broiler chickens developed respiratory disease, worsened zootechnical performance and there were no significant haematological changes. In the experiment 3, 33 E. coli isolates from ducks, turkeys, chickens and pigeons were evaluated by PFGE and antimicrobial susceptibility test to characterize their respective phylogenetic profiles and susceptibility to antimicrobials commonly used in poultry farming. E. coli originated from ducks, turkeys, chickens and pigeons grouped into a group with 41.1% similarity. Resistance to different antimicrobials tested (ampicillin, amoxicillin, ceftiofur, doxycycline, enrofloxacin, neomycin, sulfametazole, sulfonamide, tetracycline, cotrimoxazole and chloranfericol) was observed among the 33 isolates of E. coli, mainly tetracyclines, followed by fluoroquinolones and sulfonamides. It is concluded that E.coli originating from birds have different profiles and behaviors.Item Detecção de genes de virulência e suscetibilidade a antimicrobianos de escherichia coli isoladas de ovos de pata (cairina moschata)(Universidade Federal de Goiás, 2014-08-07) Almeida, Ana Maria de Souza; Jayme, Valéria de Sá; http://lattes.cnpq.br/0603234425928309; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Barbosa, Silvia Minharro; Café, Marcos BarcellosDuck is the most important species of waterfowl reared in Brazil, however, there are no studies about the risk of duck eggs in the food chain. The main objective of this study was to outline the main characteristics of waterfowl farms, integrating the risk factors associated with E. coli infection in birds and humans, verify the presence of E.coli in 38 and 25 dozen of duck eggs from markets and subsistence farms in Federal District and Goiás State, determining the pathogenic profile and the antimicrobial resistance profile. Each dozen eggs accounted for three samples: a sample pool of eggshell, pool of albumen and a pool of yolk, totaling samples.The information obtained by questionnaires applied during the visit to the farms revealed no vaccination program, or supply of balanced feed for ducks were employed. Duck were reared with chicken and the contact between the animals occurred since incubation. Through bacteriology, we isolated and identified E.coli in 17.10% (97/567) of samples of duck eggs. In PCR for the detection of the virulence genes papC, tsh, and eae and of the resistance gene iss, of 97 positives samples to E. coli, 35 samples were positive for virulence genes, 31.8% (14/44) came from farms and 39.6% (21/53) from markets. The search of genes in E.coli isolates revealed 15.4% positive to papaC, (15/97) , 21.6% (21/97) positive to tsh, 17.5% (17/97) positive to iss, and 2% (2/97) positive to eae .It conclusion, there are pathogenic strains for both birds and humans, circulating in anseriformes eggs. The investigation of antimicrobial resistance combined with the research of virulence and resistance genes in E. coli isolates obtained from eggs of ducks is an important tool to determine the risk these birds may bring to both poultry health and of public health.Item Utilização de ácido butírico encapsulado no controle de Salmnoella enterica sorovar enteritidis em frangos de corte experimental inoculados(Universidade Federal de Goiás, 2014-02-25) Araújo, Angélica Ribeiro; Stringhini, José Henrique; http://lattes.cnpq.br/8505634095383289; Porto, Regiane Nascimento Gagno; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Jardim Filho, Roberto Moraes; Leandro, Nadja Susana MojicaThe present study aimed to evaluate the encapsulated butyric acid in the control of Salmonella enterica serovar Enteritidis, performance and systemic health of broiler chickens experimentally inoculated. 576 one-day-old male chicks were distributed in a completely randomized design with eight treatments and six replications each. The poultries were inoculated orally at one-day-old with 0.3 ml of phosphate buffered saline, containing approximately 2.0 x 10 6 CFU/ml of Salmonella enterica serovar Enteritidis. Treatment 1 (T1) was the control group (Placebo); T2 - received butyric acid at a dose of 0.03%; T3 - received butyric acid at a dose of 0.075%; T4 - received the butyric acid at a dose of 0.15%; T5 -group inoculated orally (Positive Control SE); T6 - inoculated orally treated with 0.03%; T7 - inoculated orally treated with 0.075%; T8 - inoculated orally treated with 0.15%. At 22 days of age, the chickens were reinfected with 0.5 ml of phosphate buffered saline containing concentration greater than 1.0 x 10 9 CFU/ml of Salmonella. Butyric acid favored the performance up to 21 days. The acid reduced the intestinal population E. coli. The intestinal pH did not differ by butyric acid, however Salmonella caused a reduction. At 14 days, the acidification effected the growth of the villi of the jejunum and Salmonella caused a reduction in the villous and greater relation villi : crypt in the duodenum and jejunum. No Salmonella was isolated from cloacal swabs collected from poultries that received the dose of 0.03%. Salmonella reduced intestinal weight. The colonization of Salmonella in organs at eight and 28 days of age was reduced in the presence of acid. Liver weight was smaller in the inoculated poultries and larger in the non-inoculated poultries and treated with 0.03% at 15 days. The spleen was smaller than in inoculated poultries which received the dose of 0.03%, at eight days. Salmonella reduced the bursa weight at 15 days.Dietary acidification reduced the lymphoid depletion in the inoculated group. Salmonella influenced the antibody production against Newcastle Disease and Salmonella enterica serovar Enteritidis. The dose of 0.15% increased the antibody production against Newcastle Disease in the inoculated groups and antibodies against Salmonella enterica serovar Enteritidis in the non-inoculated group at 42 days. The mortality of poultries did not differ between treatments. We conclude that, among the investigated dosages, the inclusion of 0.03% yielded the best results in performance and beneficial effects on intestinal health and organ biometrics. The dose of 0.15% improved humor immunity of the poultries.Item Aditivos fitogênicos para frangos de corte experimentalmente inoculados com Salmonella enterica sorovar Enteritidis(Universidade Federal de Goiás, 2012-02-24) Barnabé, Ana Caroline de Souza; Rezende, Cíntia Silva Minafra e; Stringhini, José Henrique; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Racanicci, Aline Mondini Calil; Mascarenhas, Alessandra GimenezIn this study, 300 1 day-old male Cobb chicks were used to evaluate, with the objective of evaluating the effects of phytogenic additives on performance, gut integrity and immune system of broilers inoculated with Salmonella enterica serovar Enteritidis (SE). The birds were divided into five treatments with six repetitions each. Treatment (Ttm) 1 - group inoculated orally with 0.1 mL of sterile buffered saline 0.85% (placebo), Ttm 2 - phytogenic received additives in the feed at a dosage of 0.10 kg/ton feed of the initial age until 35 days (Contr. FA); Ttm 3 - group inoculated orally with 0.5 mL of buffered saline 0.85%, containing approximately 5.0X105 CFU/0.5mL Salmonella enterica serovars Enteritidis (Contr. positive SE); Ttm 4 - group inoculated orally with 0,5 mL of buffered saline 0.85%, containing approximately 5.0X105 CFU/0.5mL of SE and received as antimicrobial growth promoter (AGP) bacitracin methylene disalicylate at a dosage of 55 ppm in the feed until 35 days of age (SE + AGP); Ttm 5 - group inoculated orally with 0.5 mL of buffered saline 0.85%, containing approximately 5.0X105 CFU/0.5 mL SE received phytogenic additives in the feed at a dosage of 0.10 kg/ton of feed of 1 to 35 days old (SE + FA). Performance was evaluated and six birds for treatment were weighed, sacrificed and the pH of the gastrointestinal tract was measured. Fragments were collected duodenum and jejunum to histomorphometric analysis, morphometric lymphocyte count. There was significant difference in the period 1-35 of the experiment the average weight and weight gain of groups without microbial challenge, and feed conversion in the group feed with phytogenic. We observed greater higher villus height to 14 days in the duodenum and greater villus:crypt to the group that received phytogenic The pH was influenced by the AGP in the colon and caecum. There was no significant effect of treatment on biometry of organs and Salmonella groups. There was lymphoid depletion in spleen, bursa and caecal tonsils was variable, with a predominance of mild and moderate depletion. It is concluded that the additives phytogenic control the effects of SE in broilers as expressed in the performance, especially in the feed conversion, and histomorphometric of the intestine, although it has not prevented the migration of the SE for the lymphoid organs, as well as not stimulate the proliferation of lymphoid cells in broilers.Item Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás(Universidade Federal de Goiás, 2018-02-28) Calaça, Karine Louise; Jayme, Valéria de Sá; http://lattes.cnpq.br/0603234425928309; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Sola, Marília Cristina; Pôrto, Regiani Nascimento GagnoThe current study was developed to investigate the presence of Escherichia coli and Salmonella sp., to determine the antimicrobial susceptibility profile and to detect virulence genes of Escherichia coli isolates. For the study, 50 establishments were selected in Goiânia and municipalities in the metropolitan region, in which 141 samples of excreta were collected, 141 of feed, and 141 drinkers' swabs of psittacine kept in cages for resale. Samples were submitted to conventional bacteriology and PCR and Escherichia coli was identified in 9.7% (41/423) of the samples, 12% (17/141) in excreta, 8.5% (12/141) in the feed and 8.5% (12/141) in the drinkers' swabs. In the determination of antimicrobial susceptibility of Escherichia coli samples isolated, was found resistance to ciprofloxacin 4.9% (2/41), gentamicin 17% (7/41), doxycycline 34.1% (14/41), florfenicol 34.1% (14/41), trimethoprim 39% (16/41), tetracycline 41.5% (17/41), enrofloxacin 43.9% (18/41), amoxycillin 48.8% (20/41 ), neomycin 61% (25/41) and sulfonamide 90.2% (37/41). The iss gene was detected in three isolates, tsh in three, papC in two and the traT and eae genes were not detected. Salmonella sp. was not isolated in the samples. It concludes that Escherichia coli isolates show resistance to the most commonly used antimicrobials and the possibility exists that these birds are carriers of pathogenic strains.Item Caracterização do ovo de ema (Rhea americana), qualidade físico-química, microbiológica e proteômica(Universidade Federal de Goiás, 2018-05-16) Cervi, Renato Clini; Café, Marcos Barcellos; http://lattes.cnpq.br/9860968235125158; Bailão, Alexandre Melo; http://lattes.cnpq.br/5415221996976886; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Gonçalves, Maria Assima Bittar; Reis, Ângela Adamski da Silva; Rezende, Cíntia Silva Minafra e; Jaime, Valéria de SáThe commercial production of large birds in Brazil began to become significant in the 1980s. The development of emas farms for egg production represents an alternative for the diversification of farming activities, coming in line with the projects established by the FAO for the Sustainable Management of Natural Resources, which establishes the introduction of an agroecological production matrix for social and environmental sustainability. With the growing interest in the creation of wild bird species, there is an opportunity to use their eggs for consumption in food and industrial processing. Compared with laying hens eggs, knowledge of the physicochemical and microbiological properties of rat eggs is scarce, and as a consequence, the parameters of poultry eggs are often used as a model to describe eggs of ostriches, emus and greater rhea. In order to add knowledge about greater eggs for commercial use, this work was carried out with the determination of the physico-chemical composition, the profile of natural and experimental bacterial contamination with Salmonella Gallinarum, the study of albumen and gem proteins and evaluation of rheological characteristics of the eggs of emas stored for 28 days at a temperature of 10ºC and analyzed in periods of one, seven, 14, 21 and 28 days. The results obtained in the experiments determined the characteristics of the egg composition of greater, aspects of natural and experimental microbiological contamination, composition and protein degradation. The results obtained in the present experiment indicated a product differentiated in the aspects studied and corroborate for the promising growth of raticulture as a sustainable livestock activity, new raw materials for the food industry and production of high quality animal protein for human consumption.Item Pesquisa de bactérias com determinação do perfil de sensibilidade em vísceras comestíveis de frango de corte, penas e camas de aviários(Universidade Federal de Goiás, 2013-11-26) Corrêa, Fernando Augusto Fernandes; Jayme, Valéria de Sá; Nicolau, Edmar Soares; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Bueno, Cláudia Peixoto; Santin, Ana Paula IglesiasTherefore the study aimed to investigate the presence of these bacteria in edible offal, feathers and poultry litter. Relate the presence of these bacteria in different scores macroscopic changes and determine the sensitivity of E. coli and Staphylococcus antimicrobials of importance in veterinary medicine. For it was collected 13 samples of manure directly in the sheds, abattoir were collected in 12 lots of feathers, plucking directly into 12 lots of heart and liver, the inspection line, totaling 740 samples 740 samples of heart and liver. We established three scores for injuries to the heart and six for the liver. From these samples was carried out the isolation of the bacteria E. coli, coagulase positive Staphylococcus, Pseudomonas spp. and Salmonella sp.. Positive for E. coli and Staphylococcus co were tested for sensitivity to the following antibiotics: ciprofloxacin (5 mg), ceftiofur (25 mg), chloramphenicol (30 mg), erythromycin (15 mg) , tetracycline (30 mg) , trimethoprim-sulfamethoxazole (25 mg) , enrofloxacin (5 mg), oxacillin (1 mg) and gentamycin (10 mg). In addition to those for E. coli was also used colistin (20 mg) and Staphylococcus coagulase positive bacitracin (10 mg). In any sample analyzed was identified Salmonella sp. were found in hearts, 33.1%, 6.6% and 28.2% of E. coli, coagulase positive Staphylococcus and Pseudomonas spp. respectively. In livers 45.4%, 8.0% and 23.1% of E. coli, coagulase positive Staphylococcus and Pseudomonas spp. respectively. To feather the results were 29.2%, 16.7% and 35.8% of E. coli, coagulase positive Staphylococcus and Pseudomonas spp. respectively. In the samples of poultry litter was not isolating Staphylococcus isolates of E. coli and Pseudomonas spp. were 92.3% and 7.7% respectively. In antimicrobial susceptibility testing on samples of E. coli and Staphylococcus coagulase positive bacteria showed the following percentage of antimicrobial susceptibility respectively: ciprofloxacin (36.7% and 58.8%), ceftiofur (8.1% and 64.7%), chloramphenicol (81.0% and 76.5%), erythromycin (2.9% and 41.2%), tetracycline (30.9% and 41.2%), Trimethoprim -sulfamethoxazole (43.8% and 82.3%), enrofloxacin (18.6% and 47.1%), oxacillin (3.8% and 47.1%) and gentamicin (37.1% and 64.7%). For isolates of E. coli tested for sensitivity to colistin 96.7% were sensitive. And for Staphylococcus tested for sensitivity to bacitracin, no specimens were susceptible to this antimicrobial agent.Item Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte(Universidade Federal de Goiás, 2014-08-07) Figueira, Samantha Verdi; Rezende, Cíntia Silva Minafra e; http://lattes.cnpq.br/5841210447886226; Strighini, José Henrique; http://lattes.cnpq.br/8505634095383289; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Barbosa, Silvia Minharro; Café, Marcos BarcellosThe present study was developted with the main object to evaluate the invasive ability, persitence and behavior of Salmonella Schwarzengrund in embryos and broiler chicks with one day of birth. Initially, 260 fertile eggs from broiler chicken were incubated e randomly distributed onto three different incubators. In the nineteenth day post incubation, the eggs were submited to candling and at the time 40 eggs were eliminated. The remaining eggs were distrubuted and 50 eggs were inoculated with 0,2 mL of sterile buffered saline 0,85% trough the air chamber and 50 eggs were inoculated with 1,5 x 104 UFC/0,2mL of Salmonella Schwarzengrund trough the air chamber, 100 were incubated without treatment. After the eclosion, the one day-old chicks were equally distributed in four treatments, discriminated well below: T1 – feed control – 30 chicks from incubator number 1, and received 0,1 mL of sterile buffered saline 0,85% per g of feed; T2 – feed inoculated - 30 chicks from incubator number 1, received feed with 5,3 x 10 4/0,1mL/g of Salmonella Schwarzengrund in buffered saline 0,85% per g de of feed until the seventh day-old; T3- inoculated control trough air chamber -30 chicks from inoculated embryos with 0,2 mL of sterile buffered saline 0,85% trough the air chamber; T4- inoculated trough air chamber - 30 chicks from inoculated embryos with 1,5 x 104 UFC/0,2mL de Salmonella Schwarzengrund in the air chamber. Salmonella Schwarzengrund was able to cause embryo mortality, enteric colonization, negatively influenced the weight gain, septicemic and enteric signs, with no correlation between the inoculation way. The bidrs shown clinical signs seen as feces adered to cloaca,diarrhea, prostration, decreased appetite, growth retardation and sleepness. The contamination determined higher weight and length of intestinal and liver with 25 days.Therefore is conclude that Salmonella Schwarzengrund is able to invade and persist in organs of inoculated air chamber embryo and one day old chicks contaminated feed.Item Detecção dos genes spvC e prot6E e avaliação da infecciosidade de Salmonella sp. em poedeiras comerciais(Universidade Federal de Goiás, 2019-03-06) Figueira, Samantha Verdi; Rezende, Cíntia Silva Minafra e; http://lattes.cnpq.br/5841210447886226; Moraes, Dunya Mara Cardoso; http://lattes.cnpq.br/9632713129112672; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Faria, Adriana Marques; Rocha, Fernanda Rodrigues Taveira; Stringhini, José Henrique; Teixeira, Weslen Fabrício PiresThe pathogenesis of Salmonella includes different factors, such as plasmidial genes, which because they are mobile genetic mechanisms can increase the bacterial genetic diversity contributing to the modification of virulence and adaptation to the hosts. The present work was developed to detect the presence of the virulence genes spvC and prot6E in different serovars of Salmonella enteric present in the metropolitan region of Goiânia and to verify the ability of these serovars to express their pathogenicity in embryonated eggs, neonates and laying hens and to cause contamination in eggs in experimental models. In Experiment 1, isolates of Salmonella Enteritidis, Gallinarum, Heidelberg, Infantis, Schwarzengrund and Typhimurium were obtained from organs of sick bird, eggs and environment of poultry chain and were investigated the presence of spvC and prot6E genes by real-time PCR. Of the 47 isolates, 15 (31.9%) were positive for the spvC gene and five (10.6%) for the prot6E gene. It is concluded that Salmonella Enteritidis, Typhimurium and Gallinarum, isolated from the bird and eggs contain the spvC genes. The prot6E gene was detected in the serovars Salmonella Enteritidis, Heidelberg and Typhimurium from egg samples. The serovars of Salmonella Heidelberg, Infantis, Schwarzengrund and Typhimurium from environmental samples do not have the plasmid genes spvC and prot6E. In experiment 2, two isolates of Salmonella Gallinarum, one positive and one negative for the spvC gene, were inoculated into embryos and neonates of laying birds to investigate whether the presence of the spvC gene in the Gallinarum serovar is able to determine embryonic mortality, affect the quality of the neonate and chick, production parameters and cause systemic disease in young birds. It was observed high mortality of the groups inoculated with Salmonella Gallinarum in alantoid route with one and 14 days of incubation, independent of the presence of the spvC gene. No embryonic mortality, changes in neonatal weight / egg weight and changes in neonatal quality were observed for birds inoculated through a 19-day in air chamber. Salmonella Gallinarum without and with the spvC gene was recovered from the heart, spleen, liver and ceca of birds inoculated in the air chamber, whereas for the oral inoculated group, the pathogen without the gene was recovered only in the ceca and the pathogen with the gene was recovered from spleen and cecum. Salmonella inoculation led to decreased weight gain and altered biometry of the heart and intestine. It is concluded that Salmonella Gallinarum is capable of causing embryonic mortality in embryos inoculated with one and 14 days in the allantoic cavity. The presence of the spvC gene in Salmonella Gallinarum does not affect embryo mortality, incubation parameters and systemic dissemination for birds inoculated in the air chamber. For birds inoculated by the oral route, the presence of the spvC gene determines enteric and systemic infection, whereas the isolate without the gene remains restricted to the cecum. In Experiment 3, the Salmonella Heidelberg isolate positive for the prot6E gene in experiment 1 was inoculated in laying hens in the oral, intravaginal and intravenous routes in order to investigate their ability to cause clinical disease, egg and gastrointestinal tract contamination. The pathogen was isolated in the excreta, only 12 hours after inoculation in 33% of the birds inoculated from oral route and 66% of the birds inoculated intravaginal route. In the eggs, the pathogen was isolated at 24 and 48 hours, seven and 15 days. It is concluded that the presence of the prot6E gene in Salmonella Heidelberg is not sufficient to cause clinical salmonellosis in laying hens, but it determines contamination of the eggs and the gastrointestinal tract of chickens, regardless of the route of inoculation, oral, intravaginal and intravenous.Item Detecção de Salmonella sp., Mycoplasma spp. e Escherichia coli de aves sinantrópicas da região metropolitana de Goiânia-Goiás(Universidade Federal de Goiás, 2013-04-12) Hidasi, Hilari Wanderley; Linhares, Guido Fontgalland Coelho; Jayme, Valéria de Sá; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Felippe, Paulo Anselmo Nunes; Meireles, Marcelo Vasconcelos; Duarte, Sabrina Castilho; Rezende, Cíntia Silva Minafra eAves sinantrópicas aproximam-se de atividades humanas em busca de abrigo, água e alimento, podendo percorrer grandes distâncias para tanto. Em busca de informações acerca da importância dessas aves na transmissão de agentes patogênicos de importância na avicultura comercial, foi realizado estudo com 260 aves de comportamento sinantrópico, sendo 200 pombos comuns (Columba livia) e 60 urubus de cabeça preta (Coragyps atratus), na região metropolitana de Goiânia - Goiás. Foram colhidas amostras de fezes, soro e suabes traqueais que foram submetidos a testes para detecção de Salmonella sp. por bacteriologia convencional e Reação em Cadeia da Polimerase em Tempo Real (rPCR), Mycoplasma galisepticum e M. synoviae pela Soroaglutinação Rápida em Placa (SAR) e rPCR, além de isolamento de Escherichia coli pela bacteriologia convencional com detecção de genes de virulência de E. coli patogênica para aves (APEC) pela PCR e perfil de suscetibilidade a antimicrobianos dos isolados. Os resultados observados, na pesquisa de Salmonella sp. das amostras de pombos, 13% (26/200) foram positivas no exame bacteriológico e 27% (54/200) das amostras positivas no rPCR. Do total de 60 amostras obtidas dos urubus, nenhuma amostra foi positiva no bacteriológico convencional e 8,3% (5/60) foram positivas no rPCR. Para a pesquisa de Mycoplasma, das amostras colhidas dos pombos, 7,5% (15/200) amostras foram reativas no teste sorológico, sendo 4,5% (9/200) positivas para M. galliisepticum e 3% (6/200) para M. synoviae. Já no rPCR, 2,5% (5/200) foram positivas para M. gallisepticum. Das amostras colhidas dos urubus, nenhuma foi positiva nos dois testes aos quais foram submetidas. Foi isolado E. coli das excretas e detectado pela PCR os genes de virulência papC, tsh, iuc e iss, com resultado para amostras de pombos de 11,23%(20/178) para iuc, 2,24% (4/178) papC, 11,79% (21/179) tsh e 6, 17% (11/178). Para urubus 8,16% (4/49) iuc, 14,28% (7/49) tsh, 6,12% (3/49) iss, e nenhuma positiva para papC. Adicionalmente, os isolados de E. coli foram submetidos a teste de perfil de resistência à antibióticos em que se obteve: sulfametazina122/178 (68,53%), ampicilina 130/178 (73,03%), ciprofloxacina 40/178 (22,47%), apramicina 57/178 (32,02%), sulfametropin 110/178 (61,79%), enrofloxacina 71/178 (39,88%), tetraciclina 119/178 (66,85%), sulfonamida 123/172 (69,10%), neomicina 59/178 (33,14%), doxaciclina 67/178 (37,64%), oxitetraciclina 51/178(28,65%), gentamicina 42/178 (23,59%), ceftiofur 79/178 (44,38%), amoxicilina + ac. clavulânico 92/178 (51,68%) de resistência nas amostras isoladas de pombos, e em amostras isoladas de urubus: sulfametazina 36/49 (73,46%), ampicilina 39/49 (79,59%), ciprofloxacina 12/49 (24,48%), apramicina 9/49(18,36%), sulfametropin 30/49 (61,22%), enrofloxacina 7/49 (14,28%), tetraciclina 27/49 (55,10%), sulfonamida 32/49 (65,30%), neomicina 12/49 (24,48%), doxaciclina 11/49 (22,44%), neomicina 9/49 (18,36%), oxitetraciclina 11/49 (22,44%), gentamicina 10/49 (20,40%), ceftiofur 12/49 (24,48%), associação de amoxicilina e ácido clavulânico 31/49 (63,26%) de resistência. Os resultados sugerem que essas aves de comportamento sinantrópico, são potenciais veiculadores de agentes causadores de perdas na produção avícola e preocupantes para a saúde pública. Além disso, podem constituir em suporte de transferência de fenótipos de E.coli resistentes.Item Utilização do extrato etanólico de jabuticaba (Myrciaria cauliflora) no controle de Salmonella Heidelberg na produção de frangos de corte(Universidade Federal de Goiás, 2018-05-23) Leonídio, Angélica Ribeiro Araújo; Pôrto, Regiani Nascimento Gagno; http://lattes.cnpq.br/6367040339353532; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Moraes, Dunya Mara Cardoso; Leite, Paulo Ricardo de Sá da Costa; Brainer, Mônica Maria de Almeida; Carvalho, Fabyola BarrosThe objective of the present study was to evaluate the use of the ethanolic extract of of bark and seeds of Myrciaria cauliflora in the incubation of fertile eggs and in the feeding of broilers inoculated with Salmonella Heidelberg, observing possible beneficial and or protective effects of this phytogenic on health systemic effects of birds. Three experiments were carried out at the Experimental Center for Bird Diseases and Laboratory of Bacteriology of the Department of Veterinary Medicine of the Veterinary and Zootechnical School of the Federal University of Goiás. In the first experiment was evaluated the antimicrobial in vitro activity was evaluated and the minimum inhibitory concentration of ethanolic extract of M. cauliflora on Salmonella enterica different strains. In the second experiment, 320 fertile eggs distributed in randomized design were used in four treatments with 10 replicates each: CN - negative control group; EJ - group that received only the extract in the shell; SH - group that received only the bacterial inoculum in the shell; and SH + EJ - group that received the bacterial inoculum and the extract in the shell. The eggs incubated non-hatched were submitted to embryodiagnosis and processed for Salmonella in the embryos. The chicks born were housed until 10 days of age, during which time the collection and processing of samples for Salmonella research, serum biochemistry and hemogram were performed. In the third experiment, 336 one - day - old male chicks were distributed in randomized design in four treatments with seven replicates each: CN - negative control group; EJ - group that received only the vegetal extract in the diet; SH - group that received the inoculum bacterial in crop; and SH + EJ - group that received the bacterial inoculum in crop and the extract in the diet. Performance indexes, fecal excretion and presence of Salmonella in organs, organ biometry, intestinal histomorphometry, goblet cell counts and immune cells in organs and blood were evaluated. In the first experiment, the extract presented low inhibitory action on the strains of Salmonella enterica,, Salmonella Typhi, Salmonella Heidelberg and Salmonella Typhimurium. In the second experiment it was verified that the extract promoted less hatchability and higher embryonic mortality. The Salmonella isolation was higher in the embryo and meconium samples of the group that received the extract. At 10 days of age the extract reduced the excretion and colonization of the organs by Salmonella and the hematocrit and serum cholesterol level. In the third experiment it was verified that the extract increased the excretion of Salmonella in the inoculated birds and reduced the number of lymphocytes in the blood. It is concluded that the use of the ethanolic extract of bark and seeds of M. cauliflora presented weak antimicrobial activity in vitro for the strains of Salmonella enterica tested. In embryonated eggs its use is not feasible, however when added in the diet, it can stimulate the production of lymphocytes in lymphoid organs.Item Investigação bacteriológica e molecular de salmonella sp. em granjas de postura comercial(Universidade Federal de Goiás, 2014-04-11) Moraes, Dunya Mara Cardoso; Jayme, Valéria de Sá; http://lattes.cnpq.br/0603234425928309; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Stringhini, Maria Luiza; Duarte, Sabrina Castilho; Linhares, Guido Fontgalland Coelho; Mesquita, Albenones José deThe purpose of this research was to investigate presence of Salmonella sp. by conventional bacterial identification and real-time PCR on eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs; in samples of flooring material from transport crates (meconium); raising environment (swab of cages and drinking fountains); cloacal swab; food and insects from growing, rearing and production phases in a commercial group of laying hens. Also, was examinated the susceptibility profile of Salmonella isolates to 14 antimicrobials: sulfamethoxazole (25μg), trimethoprim-sulfamethoxazole (25μg), enrofloxacin (5μg), tetracyclin (30μg) sulphonamides (300μg), ciprofloxacin (10μg), amoxicillin and clavulanic acid (3μg), ampicillin (20μg), ceftiofur (30μg), gentamicin (10μg), oxytetracycline (30μg), neomycin (30μg), doxycycline (30μg) and apramycin (15μg); and evaluated the presence of virulence gene spvC and resistence genes intl1, sul1 and blaTEM. A total of 68 dozens of white eggs and 61 dozens of brown eggs were randomly collected in poultry houses and 30 dozens and 34 dozens of white and brown eggs, respectively, were collected in egg-storage room (on the farm). Each collected dozen corresponded to three sample units: eggshell, albumen and yolk, totaling 387 samples. Salmonella spp. was detected by conventional bacteriology in 5.4% of analyzed samples and in 16% by qPCR. Salmonella Agona represented 18.2% of identified serovars, Salmonella enterica subs. enterica O:4.5 18.2%, Salmonella Schwarzengrund 18.2%, Salmonella Cerro 13.6%, Salmonella Anatum 13.6%, Salmonella Enteritidis 9.1%, Salmonella Johannesburg 4.5%, Salmonella Corvallis 4.5%, Salmonella Livingstone and Salmonella Senftenberg. From 864 samples collected (248 samples originated from growing, 392 from rearing and 224 from production phase), 2,8% where positives in bacteriologic technique and 15,3% in qPCR. Contamination was higher in growing and rearing phases and declined in production phase. Twenty four isolations of Salmonella where typified as Salmonella Agona (41,7%), Salmonella Livingstone (33,3%), Salmonella Cerro (16,7%), Salmonella Senftenberg (4,2%) and Salmonella Schwarzengrund (4,2%). The highest resistance percentage observed were to sulfamethoxazole (91,0%), sulphonamides (51%) and ceftiofur (28,9%) and 0% to ciprofloxacin. Only Salmonella Johannesburg and Salmonella Corvallis showed resistance to only one antibiotic and others serovars were resistant to at least two antimicrobials, noting that Salmonella Schwarzengrund presented resistance to 13 of them. The gene spvC was dectected only in serovar Enteritidis, while intl1 was present in six of ten analyzed serovars and the gene sul1 in three of them, always in association with intl1. The gene blaTEM was not identified. In conclusion, this research points numerous serovars circulating in commercial egg structures and phenotypic antimicrobials resistance investigation, along with antimicrobial resistance genes in isolates of Salmonella sp. are important investigation tools to determinate genetic profile of these bacteria.Item Efeitos do alho (allium sativum) in natura sobre salmonella infantis experimentalmente inoculada em frango de corte(Universidade Federal de Goiás, 2015-07-15) Mota, Bárbara de Paiva; Café, Marcos Barcellos; http://lattes.cnpq.br/9860968235125158; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Hidase, Hilari Wanderley; Stringhini, José HenriqueThis study aimed to evaluate the effects of different doses of in natura garlic on the control of Salmonella Infantis and their consequence on performance, fecal excretion of Salmonella and blood parameters in experimentally inoculated broiler chickens. In this experiment we used 256 one-day-old male chicks, distributed in a completely randomized design with eight treatments, four replications with four replicates of eight birds each. The birds were orally inoculated at one-day-old with 0.5 ml of buffered saline, containing approximately 5.0 x 103 CFU / ml of Salmonella Infantis. Treatment 1 (T1) was the control group (Placebo); T2 - received garlic at a dose of 1.5%; T3 - received garlic at a dose of 3.0%; T4 - received garlic at a dose of 4.5%; T5 – was orally inoculated (Positive Control SI); T6 – was orally inoculated and treated with 1.5%; T7 – was orally inoculated and treated with 3.0%; T8 – was orally inoculated and treated with 4.5%. The performance was measured at seven, 21 and 35 days. At the days eight, 15 and 29 four birds for treatment were submitted to six hour fasting, and then they were weighed, euthanized, necropsied, and the organs were collected for weight determination and detection of Salmonella. Cloacal swabs were collected at three, 13, and 28 days and blood samples were collected at 14 and 36 days of age, after fasting for eight hours to evaluate blood parameters. At seven and 21 days, weight gain and final weight were lower in birds fed 4.5% of garlic, and there was no significant difference in the period of 1-35 days. The weight of the intestine and liver at 29 days was higher in birds inoculated and treated with 3.0% of garlic. Spleen weight was higher in birds inoculated at 29 days. Garlic did not inhibit Salmonella invasion in the spleen at the doses used. We found garlic did not reduce the intestinal E. coli population, but it reduced Salmonella. We observed garlic influenced cholesterol levels. In conclusion, fresh garlic in natura at the doses used does not alter the performance, as well as it does not inhibit the migration of Salmonella to the spleen; however, it influences serum cholesterol levels and reduces Salmonella fecal excretion of.Item Efeitos do açafrão (Curcuma longa L.) em frangos de corte inoculados experimentalmente com Salmonella Typhimurium(Universidade Federal de Goiás, 2016-05-31) Nascimento, Gisele Mendanha; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Rocha, Fernanda Rodrigues Taveira; Gomes, Natali Almeida; Moraes, Dunnya Mara Cardoso; Café, Marcos BarcellosIt was aimed to evaluate the effects of different doses of Curcuma longa in Salmonella Typhimurium control on performance, intestinal integrity, fecal excretion of Salmonella, lymphocyte count in secondary lymphoid organs, histopathology, biochemical profile and Salmonella research in organs of experimentally inoculated broilers. A total of 672 male chicks were distributed in a completely randomized design, in a 4 x 2 factorial arrangement (doses of Curcuma longa x inoculation with Salmonella Typhimurium), distributed in eight treatments and six replications. The birds were orally inoculated, on the first day of age, with 0.5 ml of buffered saline solution containing 1.2 x 104 CFU/mL of Salmonella Typhimurium. Treatment 1 consisted in the control group, with no addition of Curcuma longa and no inoculation of Salmonella typhimurium; treatments 2, 3 and 4 consisted in the groups receiving Curcuma longa at doses of 1%, 2% and 3% in the feed, respectively. Treatment 5 is the group which received only Salmonella Typhimurium inoculation; treatments 6, 7 and 8 received Curcuma longa at doses of 1%, 2% and 3% in the feed, respectively, besides inoculation of Salmonella typhimurium in the crop. It was noted statistical difference in performance, with better results in birds supplemented with 1% of Curcuma longa, and not inoculated with Salmonella Typhimurium, since the regression analysis of the final weight and weight gain showed the best dose for the two variables was 0.82%. At 21 days old, the regression showed a linear effect on weight gain and feed intake. At 35 days old, the regression showed a quadratic effect for weight gain and feed intake. The dose of 1% turmeric prevented intestinal colonization by Salmonella.Dosage and inoculation also influenced the histomorphometry and the pH of the intestinal segments. It was found, at seven days, Salmonella was isolated from the liver of birds in the control group and birds in the group treated with 1% of Curcuma longa. Salmonella Typhimurium was not isolated in the spleen, in any of the studied ages. At seven, 21 and 35 days old, birds orally inoculated with Salmonella Typhimurium had higher lymphocyte counts in the spleen when compared to not inoculated birds. There was no significant difference in histopathological evaluations of liver, kidney, and cecal tonsils. At 21 days old, there was a higher frequency of mucous in the duodenum of birds supplemented with 1 and 3% of turmeric. It was found in biochemical profile, at 14 days old, changes in levels of aspartate aminotransferase (AST), cholesterol and triglycerides, and regression analysis showed a linear effect for creatinine and aspartate aminotransferase (AST). At 36 days old, in glucose levels, uric acid, aspartate aminotransferase (AST), gamma glutamyl transferase (GGT), total protein and globulin, regression analysis showed a linear effect for total protein and globulin, and quadratic effect for uric acid. In conclusion, the inclusion of 1% of Curcuma longa in the feed improves the final weight, weight gain, feed intake and feed conversion, as well as inhibited the intestinal colonization of Salmonella Typhimurium and preserved intestinal integrity. The inclusion of 1% has also altered the biochemical profile, with a reduction in cholesterol levels.Item Ocorrência de Salmonellasp. e características físicas de poedeiras em fase de descarte(Universidade Federal de Goiás, 2014-03-25) Oliveira, Édilon Sembarski de; Santana, Eliete Souza; Café, Marcos Barcelos; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Parente, Leila Maria Leal; Stringhini, José Henrique(Sem resumo)Item Genes de virulência em Escherichia coli isolada de frangos de corte de criações industriais e alternativas(Universidade Federal de Goiás, 2013-12-13) Rocha, Tatiane Martins; Stringhini, José Henrique; Nunes, Iolanda Aparecida; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Meireles, Marcelo Vasconcelos; Coelho, Karyne Oliveira; Café, Marcos Barcelos; Linhares, Guido Fontgalland(Sem resumo em outra língua)Item A vigilância de primatas não humanos e a febre amarela silvestre no Brasil(Universidade Federal de Goiás, 2012-09-19) Romano, Alessandro Pecego Martins; Jayme, Valéria de Sá; Marinho, Maria Lucia Gambarini; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Resende, Cintia Silva Minafra e; Linhares, Guido Fontgallad Coelho; Araújo, Wildo Navegantes; Obara, Marcos TakashiThis study sought to describe the use of surveillance of non-human primates in surveillance of Sylvatic Yellow Fever and evaluate the usefulness in the prevention of human cases in Brazil. The epizootic surveillance began its records in 1999 and expanded the activity and the number of notifications after 2006, when the deaths or illnesses of nonhuman primates came to be considered an event of epidemiological relevance and therefore compulsory notification, as currently defines the ordinance 104/GM/MS/2011. 2748 epidemics were recorded in non-human primates in Brazil. The confirmation of yellow fever occurred in 8.8% of notifications. It was characteristic of yellow fever during the study period, two epizootic epidemic periods, with dispersal occurring from areas of Northern and central west with subsequent spread to the South and Southeast regions. During the study period yellow fever hit areas unvaccinated populations where the activity of epizootic surveillance helped to identify affected areas preventing human cases were recorded. The surveillance of NHP revealed the involvement of other infectious agents possibly involved in epizootics reported and highlights the potential contribution of this surveillance strategy for other infectious causes of public health importance and has been identified as seropositive for other arboviruses in primate populations in free life, demonstrating that this surveillance strategy animal may be useful to other map areas of occurrence of other arboviruses. The surveillance strategy allowed animals to detect areas of circulation of YF virus and direct vaccination campaigns, especially in outbreak situations, preventing human cases of the disease and demonstrating its utility and contribution to the monitoring of YF. This monitoring strategy can be enhanced by broadening their purposes as may possibly contribute to the surveillance of other diseases as an important interface of human and animal health.Item Inoculação experimental de Salmonella Enteritidis em perus e controle da infecção com o uso da lactulose na dieta(Universidade Federal de Goiás, 2011-09-12) Santana, Eliete Souza; Stringhini, José Henrique; Pôrto, Regiani Nascimento Gagno; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Luna, Antonio Muñoz; Parente, Leila Maria Leal; Rezende, Cintia Silva Minafra e; Café, Marcos BarcellosThree experiments were performed aiming to elucidate aspects concerning the pathogenesis, clinical, pathological and immunological changes in turkeys inoculated with Salmonella Enteritidis, as well as the control and performace variables, with the use of lactulose. Experiment 1 consisted of three treatments and a control group. A second group inoculated with 6.0x102 CFU/mL and a third one inoculated with 7.0x105 CFU/mL of Salmonella Enteritidis. Within 1, 3, 6, 12, 18 and 24, and three, four, 38 and 49 days post-inoculation, two birds per treatment were sacrificed and vitelline sacs, spleen, bursa of Fabricius and ceca were coleted for Salmonella research, lymphocytes levels, and immunohistochemistry. The pathogen was isolated in organs studied in both tests and the lymphocytes levels decreased. Was observed that the dose influences the penetration, dissemination, and persistence of the pathogen elimination in turkeys. In Experiment 2, 160 turkeys were distributed into four treatments within 6.0x102CFU/mL, 7.0x105 CFU/mL, 8.0x109 CFU/mL of Salmonella Enteritidis and a control group. With 1, 3, 6, 12, 18 and 24 and three, four, 38 and 49 days were sampled blood, liver, spleen and bursa of Fabricius of two birds per treatment for electrophoretic, bacteriological and histopathological analysis, and also lymphocyte levels. There were differences in total serum protein data, as well as in bacterial isolation and tissue changes from 6 hours to 4 days before. The decrease of lymphocytes levels were registered from 3 hours post-inoculation. High mortality levels were observed in all groups in the first week, of which the main clinical and histopathological changes, as well the mortality levels were more evident in the group that received the highest dose. Was observed that serum proteins values, liver histopathological lesions, and decrease of lymphocytes levels occurs primarily in the first days post-infection. Experiment 3 consisted of 280 turkeys distributed in four treatments: placebo, control of lactulose; inoculated with 7.0x105 UFC/mL of Salmonella Enteritidis and inoculated with 7.0x105 UFC/mL of Salmonella Enteritidis and treated with lactulose. At seven, 21, 42 and 60 days were performed analisis of accomplishment. One bird of each quota was sacrificed and collected duodenum and jejunum from each for histomorphometric analysis, and ceca and inglúvios for enumerations of Enterobacteriaceae, Lactobacillus, Salmonella, pH measurements and biometric examination of intestines. Still, were collected liver, spleen, bursa of Fabricius and the gastrointestinal tract for bacteriological, histopathological, lymphocyte levels, and biometric examination. Were observed a lower weight gain in the group that received only the pathogen and increased only in patients that receiving lactulose. Were observed a higher correlation villous: crypt in the duodenum and jejunum of lactulose and placebo groups at seven and 21 days. Higher levels of UFC of Lactobacillus and Enterobacteriaceae were observed in the groups that receivied lactulose. Salmonella Enteritidis infections determine histopatological changes, decrease of lymphocyte levels and mortality. Lactulose increases beneficial bacteria, reduces the colonization of Salmonella, Promotes intestinal development and improved the animal performance, minimizes lymphocyte and tissue changes, reducing mortality and carrier state.Item Caracterização molecular de genes isolados de Escherichia coli de salpingites e dermatoses em aves ao abate(Universidade Federal de Goiás, 2016-07-17) Silva, Jayane Ricardo Monteiro da; Rezende, Cíntia Silva Minafra e; http://lattes.cnpq.br/5841210447886226; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Barbosa, Sílvia Minharro; Oliveira, Cairo Henrique de SousaAs infecções causadas por Escherichia coli patogênicas para aves são apontadas como uma das principais causas de impactos econômicos para a indústria avícola pelo retardo de crescimento e condenação de carcaças de aves nos matadouros. O presente trabalho foi desenvolvido com o objetivo de identificar Escherichia coli de amostras de salpingite e dermatose de poedeiras e frangos de corte, respectivamente, bem como detectar os genes de virulência de estirpes de APEC e identificar sorogrupos. Para tanto, 620 amostras de frangos de corte e poedeiras, com e sem lesões, condenadas parcial ou totalmente ao abate foram coletados em dois matadouros. As amostras coletadas foram submetidas inicialmente a bacteriologia e isolados de E. coli foram processados por PCR para detectar a presença de genes de virulência e resistência, e posteriormente submetidas a análise sorológica para cinco sorosgrupos. Os resultados mostraram um total de 18,06% (112/620) isolados de APEC, sendo 61,60%(69) para salpingite e 38,40%(43) para dermatose. Na pesquisa dos genes, não foram encontradas presença do gene sfa e hly em nenhuma das amostras (0/112), enquanto que o gene traT foi identificado em 80,36% (90/112) e o iss em 55,36% (62/112). Detectou-se a presença simultânea dos genes iss e traT nos isolados. Para a sorotipagem, observou-se uma frequência baixa para E. coli enteropatotgênica (EPEC), E. coli enteroinvasora (EIEC), E. coli O128 e E. coli O167, e um número considerável de E. coli enterohemorrágica O157:H7, 3% para salpingite e 5,9% para dermatoses. Conclui-se que houve um índice relevante de isolados de E. coli das amostras estudadas bem como a presença dos genes iss e traT.