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Item Estudo comparativo entre dois critérios de classificação histológica, contagem de agnor’s e expressão de c-kit no mastocitoma canino(Universidade Federal de Goiás, 2013-09-27) Borges, Juliana Carvalho de Almeida; Moura, Veridiana Maria Brainezi Dignani de; Prado, Yandra Cássia Lobato do; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; Miguel, Marina Pacheco; Damasceno, Adilson DonizetiIn dogs, mastocytoma is the most common skin cancer, representing up to 21% of all skin tumors. Although the most consistent prognostic factor in diagnosis is histopathological evaluation, different classification systems for canine cutaneous mast cell tumors are used, reducing the prognostic value of histological grading. The Patnaik system, based on a three-level classification, is still the most used, despite its subjectivity. In 2011, KIUPEL and collaborators proposed a new histological classification system, sorting the tumors into two classes, low intensity and high intensity. The objective of this study was to evaluate the comparison between the methods of classification of Patnaik and Kiupel, and establish a possible relationship between the biological behavior of Kiupel method classified mastocytoma with the through the histochemical AgNOR's technique and the expression of c-KIT. Samples of canine cutaneous mastocytoma from the Laboratory of Animal Pathology, School of Veterinary and Animal Science, Federal University of Goiás were used for this purpose. In addition to highlighting the subjectivity of tumors classified as intermediate grade system by Patnaik, the analysis indicated that the Kiupel’s method brought up greater agreement among pathologists, confirming its efficiency. The proliferative index determined by AgNOR's was greater in high intensity mastocytomas. In general, the expression of c-KIT corroborated the histological classification. The results showed that the new Kiupel classification system, is more efficient for the histological diagnosis and prognosis of canine mastocytoma when coupled with immunohistochemical and histochemical study.Item Atividade citotóxica do extrato etanólico da casca de pequi (Caryocar brasiliense) em células de osteossarcoma canino in vitro(Universidade Federal de Goiás, 2016-03-04) Braga, Karla Márcia da Silva; Bianchi Filho, Cesario; http://lattes.cnpq.br/1927380763523677; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; Damasceno, Adilson Donizeti; Prado, Yandra Cássia Lobato doOsteosarcoma is a mesenchymal high mortality tumor of dogs and men. Cytotoxicity assays employing tropical plants have been performed in order to identify possible active compounds able to inhibit neoplastic cells proliferation. The fruits from the Cerrado, Center-west Brazil’s diversity-rich, predominant vegetation, are well-known by the general public but very little is known about their pharmacological properties. Thus, the aim of this study was to verify the cytotoxic activity of the ethanoic extractItem Hemostasia e reação tecidual ao implante de esponja de quitosana em lesão hepática de camundongos(Universidade Federal de Goiás, 2015-06-30) Costa, Flávia Resende Martins da; Filice, Letícia de Souza Castro; http://lattes.cnpq.br/9213076488588229; Fioravanti, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves; Carneiro, Jussara de Souza; Jacomini, José Octavio; Martins, Danieli Brolo; Santin, Ana Paula IglesiasChitosan is a polysaccharide amino derivative of chitin, and constitutes most of the insects and crustaceans exoskeletons and fungal cell wall, thus being a low-cost, renewable and abundant, biodegradable natural product. Biological characteristics, biocompatibility and biodegradability allows various uses of this biomaterial in healthcare. The present study aimed to evaluate the hemostatic contact effect and tissue response to the implant of chitosan-alginate sponge in hepatic lesion in Swiss mice, comparing to freeze-dried hydrolyzed collagen sponges. The lesions were surgically induced. The hemostatic contact effects of chitosan and hydrolyzed collagen sponges were evaluated through its mechanical compression on hemorrhagic lesions, while the effects of his implants were observed from the incorporation of fragments of those sponges in liver failures. Gauze sponges were used as control for contact study. For the implants evaluation, liver segments removed to produce hepatic injury were used as controls. Average hemostasis times for chitosan and collagen treatments were 49 seconds, and one minute and 28 seconds for gauze treatments. Grossly, omental adhesions were observed on the implants and faster integration of collagen sponge at receiver tissue. Microscopic evaluation showed inflammatory reaction to both implanted materials and collagen synthesis stimulation. Average percentage of collagen from hepatic recipients segments of the chitosan-alginate sponge implant, and collagen hydrolyzate sponge implant increased significantly between 7 and 14 days postoperatively, pointing to a stimulating effect exerted by chitosan sponges for tissue repair. In conclusion, chitosan sponges have hemostatic action and stimulating effect on the synthesis of hepatic collagen fibers.Item Análise química de extratos de Caryocar brasiliense com potencial antioxidante(Universidade Federal de Goiás, 2019-03-01) Ferreira, Claudiane Marques; Cruz, Vanessa de Sousa; http://lattes.cnpq.br/8788967925940484; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; Terezan, Ana Paula; Araújo, Luciana Batalha Miranda deThe pequi (Caryocar brasiliense) is a typical Brazilian fruit that stands out for its high nutritional value, besides its sensorial attributes like flavor, aroma and color. The fruit peel is a byproduct of industrial processing and has potential for explotation due to the presence of phenolic compounds that act in the animal body as antioxidants, capable of preventing or preventing the action of free radicals that cause degenerative diseases. The objective of this work was to analyze the presence of phenolic compounds and their antioxidant activities through chemical analyzes of crude ethanolic extract and its hexane, dichloromethane, ethyl acetate and aqueous fractions of Caryocar brasiliense bark. The identification of phenolic compounds was performed by high performance liquid chromatography coupled to high resolution mass spectrometry. Phenol content was analyzed by determination of the total phenolic profile by the Folin-Ciocalteu method and the antioxidant capacity was analyzed by the in vitro antioxidant activity method by the DPPH assay. A total of fifteen phenolic compounds were identified by comparison with the standards: gallic, ellagic, protocatechic, gentilic, vanillic, caffeic, ferulic and p-coumaric acids. In addition to catechin, epicatechin, luteolin, kaempferol, quercetin, rutin and naringerin. The dichloromethane, ethyl acetate and aqueous fractions showed thirteen of the fifteen phenolic compounds, the crude extract twelve and the hexane ten. The total phenol profile ranged from 0.6 to 48 mg / 100 mL and the in vitro antioxidant activity showed high values ranging from 26% to 99.4%. The results confirm that the crude extract of the bark of the Caryocar brasiliense fruit and its hexane, dichloromethane, ethyl acetate and aqueous fractions have phenolic compounds and antioxidant activity.Item Bases ósseas e musculares dos cortes comerciais do pescoço, tronco e cauda de jacaré-do-pantanal (Caiman yacare Daudin 1802)(Universidade Federal de Goiás, 2013-10-18) Figueiredo, Sandra Izilda Souza de; Araújo, Luciana Batalha de Miranda; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795286E3; Ferraz, Rosa Helena dos Santos; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796004J7; Araújo, Eugênio Gonçalves de; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784181D1; Araújo, Eugênio Gonçalves de; Machado, Márcia Rita Fernandes; Santos, André Luiz Quagliatto; Cardoso, Júlio Roquete; Pereira, Kléber FernandoYacare caiman (Caiman yacare) meat consumption has become a marketing trend and a commodity on the rise in Mato Grosso state in Brazil. Commercial exploitation was regulated in 1990, a fact that boosted scientific investigation on the various aspects related to this species. Since characterization of muscle and bone bases of commercial cuts from the yacare caiman has never been reported and is pivotal for the meat industry, it became the main goal of this work. To describe the bones, six boned carcasses from juvenile yacare caimans were used, as well as an adult specimen, obtained by donation after death from the Federal University of Mato Grosso Zoo. Bones were macerated, bleached and anatomical details recorded. To study the muscle, 24 juvenile specimens were obtained after slaughter and skinning, frozen and dissected on both sides immediately after thawing. At the neck region, the only commercial cut is the back sirloin, which has as main base the nine cervical vertebrae, associated to their ribs, and formed by neck muscles, except for the intertransverse cervical and external intercostal cervical muscles. Three cuts are present at the dorsal region. The sirloin cut consists of the semispinal, longissimus and iliocostalis muscles, inserted on thoracic vertebrae and ribs, as well as lumbar and sacral ribs. The meat trims cut is formed by latissimus dorsi, serratus, pectoral and abdominal (external oblique, internal oblique, transversus and rectus) muscles, based in various bones: bone ribs are the thoracic, lumbar, and sacral ribs, the gastralia, the sternum and epipubis. The filet mignon cut is formed by the internal puboishial femoral cranial (sublumbar) muscle and by the troncocaudal (ventral surface of the pelvis) muscle. The commercial meat cuts of the tail are the tail sirloin, composed of semispinal caudal, longissimus caudal, ilioischiocaudal and long caudofemoral muscles, transverse and deep of the tail, as well as the tail tip, consisting of the longissimus caudal and ilioischiocaudal muscles, based on last five or six coccygeal vertebrae. This study on the anatomy of bones and muscles of the commercial cuts from the Caiman yacare is likely to support meat cuts standardization for this species, improving product outcome.Item Propriedades citotóxicas da Beta-Lapachona em células de osteossarcoma in vitro(Universidade Federal de Goiás, 2017-03-09) Gabriel, Gabriela Hadler; Bianchi Filho, Cesário; http://lattes.cnpq.br/1927380763523677; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Oliveira, Cairo Henrique Sousa de; Cruz, Vanessa de SousaOsteosarcoma is the main primary bone tumor, with unfavorable prognosis, high mortality and high incidence of metastases. The treatment of choice is the removal of the tumor associated with combined chemotherapy, whose adverse effects allude to the increasing need to develop new drugs. The plants constitute a large natural reserve of compounds that have medicinal and therapeutic properties, such as lapachol and its derivative, β-lapachone. The aim of this study was to evaluate the cytotoxic effects of β-lapachone in osteosarcoma cells cultured in vitro. Cells were cultured and treated with β-lapachone at different concentrations and times of exposure. Tripan blue exclusion, tetrazolium reduction and cell survival assay methods were performed to evaluate the effects of the compound on the cells. Cells treated with 0,1μM β-lapachone showed lower initial cytotoxicity in the 24h time, whereas those submitted to 1,0μM showed less viability after 72h of treatment. Cytotoxicity increased as the concentration and time of exposure increased. The lowest IC50 (0,148μM) was observed in treated cells for 72h. Cell growth after treatment was lower in the 1.0μl group after 72h and the highest cell growth was observed under a concentration of 0.1μl after 24h. There was no difference between groups for cell proliferation after treatment, and the cell survival fraction was lower after 72h of exposure. It was concluded that β-lapachone has cytotoxic effects on osteosarcoma cells cultured in vitro.Item Ação citotóxica e antioxidante da beta lapachona em células endoteliais(Universidade Federal de Goiás, 2017-12-18) Gonçalves, Patricia de Almeida Machado; Cruz, Vanessa de Sousa; http://lattes.cnpq.br/8788967925940484; Pereira, Kleber Fernando; http://lattes.cnpq.br/4779531102983799; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; Santin, Ana Paula Iglesias; Porto, Regiani Nascimento GagnoOxidative stress is the overproduction of reactive oxygen species that cause cell damage and even the death. It is a mechanism related to ischemia and reperfusion, a process that affects several organs and is involved in neurodegenerative diseases, cardiac and/or renal insufficiencies, hepatic dysfunction, among others. The discovery of new antioxidant substances derived from natural sources, is important for the prevention and treatment of these diseases. Beta lapachone is a substance with antioxidant action obtained from sawdust of Ipe wood, a symbolic tree from the Brazilian cerrado. This study aimed to analyze the cytotoxic and antioxidant action of beta lapachone in endothelial cells, in vitro. EA.hy 926 lineage cells were subcultured, treated with different concentrations of beta lapachone and subjected to ischemia and reperfusion. Cytotoxicity and antioxidant action were assessed through the cellular viability analysis by the tetrazolium reduction method. The averages were verified by the Tukey test (5% of significance) and by variance analysis. There was a dose-dependent progression of cytotoxicity in the non-ischemia/reperfusion (control) group and cell viability increase in the ischemia/reperfusion group. However, there was no statistical difference between the concentrations and between groups. Considering the settings of this experiment, Beta lapachone did not present cytotoxic or antioxidant action on endothelial cells.Item O extrato etanólico da casca de pequi reduz o dano cerebral induzido em ratas submetidas à dieta hipercalórica(Universidade Federal de Goiás, 2017-03-17) Mendes, Fernanda Figueiredo; Fioravanti, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; Prado, Yandra Cássia Lobato do; http://lattes.cnpq.br/4251555870704843; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Leite, Liliana Borges de Menezes; Custódio, Carlos Henrique Xavier; Pimenta, Vanessa de Souza Cruz; Damasceno, Adilson DonizetiThe aim was to determine the effect of ethanolic extract of pequi mesocarp on induced brain damage and ERK1/2 and AMPKα active forms in rats subjected to a hypercaloric diet. 48 rats were sorted into two groups of 24 according to the diet used, hypercaloric or commercial, provided daily for 60 days. The animals were separated into two subgroups of 12, treated or not with the extract, given daily for 30 days after diet start. Quantification of triglycerides, induction of global cerebral ischemia followed by reperfusion was performed. Later, euthanasia, brains harvest and visceral fat weighing were performed. Histopathological evaluation of brain lesions, quantification of the number of viable and non-viable cells in the cerebral cortex and hippocampus, and of cells marked by the anti-pKR-ERK1/2 and p-AMPKα antibodies in the cerebral cortex were performed. Hypertriglyceridemia and a significant increase in the amount of visceral fat were observed in the group that received hypercaloric diet (p <0.05). Histopathological evaluations showed that the group that received hypercaloric diet and was treated with the extract had fewer brain lesions of ischemia and reperfusion. The extract did not influence the number of viable and nonviable cells in the cerebral cortex and hippocampus, but significantly reduced p-ERK1 / 2 and p-AMPKα cell labelling in the hypercaloric diet group (p <0.05). In conclusion, ethanolic extract of pequi peel reduces induced brain lesions in rats fed a hypercaloric diet and has a modulatory effect on the expression of ERK1 / 2 and AMPKα in the cerebral cortex.Item Efeitos do plasma rico em plaquetas na expressão de metaloproteinases MMP-2 e MMP-9 no líquido sinovial de equinos com sinovite induzida(Universidade Federal de Goiás, 2019-06-26) Peixoto, Andréia da Costa; Brandstetter, Luciana Ramos Gaston; http://lattes.cnpq.br/0184140058949751; Moura, Veridiana Maria Brianezi Dignani de; http://lattes.cnpq.br/8773201078957745; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Moraes, Júlia de Miranda; Araújo, Eugênio Gonçalves de; Noronha Filho, Antônio Dionísio FeitosaThe equine musculoskeletal system suffers contant overload, what increases its predispositon to injuries, particularly the development of joint diseases. Conventional anti-inflammatory treatments have both temporary and symptomatic effects, as well as unwanted side effects. Platelet Rich Plasma (PRP) has been studied as a regenerative cellular therapy for joint diseases as it aims the modulation of extracellular matrix degradation and regeneration (MEC) factors. Important degradation factors are the metalloproteinases (MMPs), especially MMP-2 (Matrix metalloproteinases type 2) and MMP-9 (Matrix metalloproteinases type 9) in equines. Therefore the aim of this dissertation is to review the elements involved in the arthropathies, and investigate the intra-articular effects of PRP on the MMP-2 and MMP- 9expression. Twelve healthy horses were divided into groups: Control and Treatment (CG and GT). Induction of synovitis was performed in all horses by means of lipopolysaccharide (LPS) injection into the left radiocarpal joint. GT was treated with a single intrasinovial PRP injection. PRP was obtained by double centrifugation method. The expression of MMP-2 and MMP-9 in the synovial fluid was determined by ELISA test. Density, pH, glucose, protein, red blood cells and nucleated cells were also determined. These analysis were done prior to and after induction of synovitis and six, 24 and 48 hours after PRP injection. Regarding MMPs expression, there was no difference between groups (p> 0.05), as well as in the evaluation of glucose, red blood cells and nucleated cells. However, density, pH and proteins were different in the comparison between the two groups at six hours post PRP treatment (p = 0.0057; p = 0.0233; p = 0.0152, respectively). In the experimental model of induced synovitis in horses, PRP seems to have an influence on MMP-2 levels, but not on MMP-9, although it was not statistically observed. In addition, it also influenced the physico-chemical and cytological aspects of LS.Item Propriedades citotóxicas daβ lapachona em células de osteossarcoma caninoin vitro(Universidade Federal de Goiás, 2015-03-27) Pimenta, Vanessa de Sousa Cruz; Fioravanti, Maria Clorinda Soares; Prado, Yandra Cássia Lobato do; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; Bianchi Filho, Cesario; Miguel, Marina Pacheco; Araújo, Luciana Batalha de Miranda; Damasceno, Adilson DonizetiOsteosarcoma is the most diagnosed primary bone cell tumor in dogs and humans. The need for more effective drugs with less intense collateral effect has triggered the development of plant-derived, natural-source chemotherapeutics. This study aimed to verify β lapachone intracellular effects on canine osteosarcoma cultured cells, as well as identify action mechanisms related to its antiproliferative properties. Cells were obtained from a cell line bank, sub cultivated and subjected to treatment with different β lapachone concentrations, followed by tetrazolium reduction, Tripan Blue dye exclusion assay, clongenic survival assay, Annexin V-FITC and propidium iodine double-labeling, JC-1 dye labeling and cell cycle kinetics analysis. The group treated with β lapachone for 72 hours showed the lowest cell viability, 27,74%, and the most conspicuous citotoxic effect, 64,81%, at 0,3 μM concentration; lower IC50, 0,180 μM and also the lowest cell growth - 0,50%- following treatment with 1,0 μM concentration. No statistical difference for cell proliferation was verified between concentrations after β lapachone exposure.Early apoptosis was the most frequent type of cell death considering all groups. It was less frequent in the 24-hour group treated with 0,1 μM (4,26 %) and more frequent in the 72-hour group treated with 1,0 μM (85,89 %). Mitochondrial depolarization was dose-dependent. Cell growth inhibition was carried out through cycle block at G0/G1 phase, according to exposure time. β lapachone was shown to have antiproliferative and cytotoxic effects, to induce apoptosis and to block cell cycle at G0/G1 phase on canine osteosarcoma cells.Item Permeabilidade intestinal, translocação bacteriana e ocorrência de osteomielite vertebral em frangos submetidos ao estresse entérico(Universidade Federal de Goiás, 2018-02-08) Rodrigues, Denise Russi; Café, Marcos Barcellos; http://lattes.cnpq.br/9860968235125158; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; Rocha, Fernanda Rodrigues Taveira; Gomes, Natali Almeida; Cruz, Vanessa de Sousa; Laboissiere, MicheleVertebral osteomyelitis is an emerging disease in the world poultry industry, characterized by immobility and mortality of broilers and breeders chicken due to the infectious process in the fourth thoracic vertebra (T4). The aim of this study was to develop an experimental model induced by enteric stress to better understand the pathogenesis of vertebral osteomyelitis, as well as to investigate the role of enteric stress in intestinal permeability and bacterial translocation to liver, spleen and vertebral column of broiler chickens. Enterococcus cecorum strains (11 TXs and 11 TXb) that presented the virulence genes virulence factors: capsular polysaccharide I and II, enterococcal polysaccharide antigen M and P, protein lipoate synthase and surface protein LPTXG3 were inoculated after enteric stress induced by the use of dexamethasone for seven days in the diet (DEX), dried distilled grain with solubles - DDGS (30%) in the diet and the 24-hour food restriction (RA). The macroscopic findings of lesions responsible for claudication affected 19.37% (186/960) of the birds submitted to enteric stress and 9.06% (87/960) had vertebral osteomyelitis. The group that presented enteric inflammation induced by DDGS in the diet had an increase (p<0.05) in the incidence of vertebral osteomyelitis and lameness. The intestinal permeability, as assessed by serum FITC-d levels, increased (p<0.05) in DEX at 16, 23 e 30 days of age and RA at 30 days of age. After inoculation of E. cecorum, there was an increase (p<0.05) in microaerophilic bacteria in the liver and spleen in the DEX group at 20 days. Likewise, an increase of these bacteria was observed in T4 in the RA group at 27 days of age and in the DEX group at 34 days. Bacterial isolation identified a diversity of bacteria as species of Enterococcus, Streptococcus, Staphylococcus, Lactobacillus, as well as Escherichia coli in T4, suggesting that other microbial agents besides E. cecorum may be involved in vertebral osteomyelitis lesions. Therefore, the experimental reproduction model of vertebral osteomyelitis induced by enteric stress in broilers makes it possible to study vertebral osteomyelitis lesions, which favors its use in the applied research of preventive and therapeutic strategies for this disease. In addition, it is concluded that enteric stress increases intestinal permeability and promotes the translocation of opportunistic bacteria to the liver, spleen and spine of broiler chickens, which may lead to the development of vertebral osteomyelitis in broilers.Item Detecção molecular de herpesvírus bovino tipo-1 e herpesvírus bovino tipo-5 em amostras de encéfalos bovinos incluídos em parafina(Universidade Federal de Goiás, 2014-09-04) Silva, Danilo Rezende e; Linhares, Guido Fontgalland Coelho; Cunha, Paulo Henrique Jorge da; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; Miguel, Marina Pacheco; Prado, Yandra Cássia Lobato doIdentification of herpesvirus in specimes fixed in formalin and paraffin embedded has been used in experimental analysis. Several molecular techniques have been used in research and in diagnosing of herpesviral meningoencephalitis. 80 cases of routine in veterinary pathology were reviewed on the Department of Animal Pathology of Veterinary and Science Animal School Medicine of Federal University of Goias, Goiania, GO (EVZ/UFG) performed between January 2007 to December 2012. Were used 18 cases with a total of 44 paraffin blocks (Group I - 14 blocks, Group II - 17 blocks and Group III - 13 blocks), which were grouped according to histopathological diagnosis, in Group I - samples without histological changes and no clinical history of neurological disease, Group II - samples with nonspecific encephalitis, Group III - samples with rabies encephalitis. Were extracted DNA fragments, from cuts blocks, using a commercial extraction kit of formalin and paraffin embedded tissue. Afterwards the technique standardization of conventional PCR for bovine herpesvirus-1 and bovine herpersvírus type-5 was performed. Were positive for BoHV-5 28% (5/18) of cases, with 11% (2/18) of the group with nonspecific encephalitis and 17% (3/18) of the group with encephalitis and positive for the rabies virus. In the test for checking of the bands for the BoHV-1, all samples, 100% (18/18) were negative. These results demonstrate that the conventional PCR technique is helpful in aiding the definitive diagnosis of encephalitis bovine herpesvirus type-5.Item Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro(Universidade Federal de Goiás, 2018-06-29) Silva, Nara Cristina; Cruz, Vanessa de Sousa; http://lattes.cnpq.br/8788967925940484; Damasceno, Adilson Donizeti; http://lattes.cnpq.br/3900110295277130; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Pôrto, Regiani Nascimento Gagno; Santin, Ana Paula Nascimento Iglesias; Araújo, Eugênio Gonçalves deOsteosarcoma is a highly aggressive malignant bone tumor that has an unfavorable prognosis. The treatment of choice is surgical intervention associated with systemic chemotherapy. However, the chemotherapeutic agents currently employed have several adverse effects on patients that limit their use. Thus, research aimed at new alternatives to the treatment of this cancer is of paramount importance. This study aimed to evaluate, in vitro, the cytotoxic potential of extracts from leaves of gabirobeira in osteosarcoma cells. From the leaves of this plant, the crude ethanolic extract and ethyl acetate were extracted. The cells were cultured and treated with both extracts at different concentrations and at predetermined exposure times. Cell cytotoxicity was analyzed by the Tripan blue exclusion assay and the IC50 calculated by the tetrazolium reduction method. The crude extract presented higher cytotoxicity compared to acetate, and the best result was in the 1.0μL/mL group treated for 48 hours. In addition, the IC50 value for G48 is within the range suggested as the best cytotoxicity. Finally, extracts of the leaves of the gabirobeira presented cytotoxic action in the cells of in vitro osteosarcoma.Item Efeitos dos extratos de folhas de campomanesia adamantium e hymenaea martiana hayne sobre células de osteossarcoma canino e células endoteliais humanas(Universidade Federal de Goiás, 2020-02-28) Vieira, Vanessa de Souza; Cruz, Vanessa de Sousa; http://lattes.cnpq.br/1222753642148629; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/391977757005992; Araújo, Eugênio Gonçalves; Pfrimer, Gabriel de Abreu; Pereira, Kleber Fernando; Arnhold, Emmanuel; Oliveira, Gerlon de Almeida Ribeiro deOsteosarcoma (OSA) is a very aggressive tumor in dogs, with low survival rate and ineffective treatment. In the search for alternative sources of therapy, the Brazilian biome becomes a scientific hope, presenting a diversity of medicinal plants of popular knowledge and use. However, they can have controversial biological functions. The aim of this study was to verify the bioactivity of the leaves of Campomania adamantium and Hymenaea martiana Hayne on canine osteosarcoma (OC) cells from cell cultures and human umbilical vein endothelial cells (HUVEC). As cells were cultured and subjected to treatment with C. adamantium (1 μg / mL, 10 μg / mL, 100 μg / mL, 1000 μg / mL) and H. martiana Hayne (10μL / mL, 100μL / mL, 1000μL / mL , 2000μL / mL and 5000μL / mL), in the 24h, 48h and 72h exposure periods in normoxia and 24h in cells exposed to oxidative stress, induced by hydrogen peroxide. The results were analyzed by analyzing cell viability and cytotoxicity using the tetrazolium reduction method (MTT). This study brings unprecedented results in relation to extractive effects, as it was possible to demonstrate that they have little cytotoxic action. In addition, it was observed that the higher the dosage and the longer the exposure time, the greater the proliferative activity, with increased cell viability in the 72-hour group. In endothelial cells, similarly, there was no cytotoxic activity, in addition to the occurrence of increased cell viability, even after submission to oxidative stress. C. adamantium leaf extract increased viability and did not show cytotoxic action in canine osteosarcoma cells and endothelial cells under oxidative stress. The crude ethanolic extract of the leaves of Hymenaea martiana Hayne, increased the cell viability of canine osteosarcoma cells and human umbilical vein endothelial cells, submitted to oxidative stress.