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Item Produção e manejo de resíduos de serviços de saúde gerados em hospital veterinário(Universidade Federal de Goiás, 2010-02-12) ALVES, Cláudia Bueno; TIPPLE, Anaclara Ferreira Veiga; http://lattes.cnpq.br/4288704233343920; JAYME, Valéria de Sá; http://lattes.cnpq.br/0603234425928309; SILVA, Olízio Claudino da; http://lattes.cnpq.br/7787082855013504Usual practices in health stablishments, both human and animal, produce a considerable quantity of residues that potentially represents risks to health of the internal community and the population. These wastes demand special management, with or without treatment prior to the final destination. Consequently, the processing constitutes a vital topic to occupational safety of the employees who handling them, such as to the public health and environment. Thus, this study aimed to analyze aspects of the control of residues produced at the Veterinary Hospital of the Federal University of Goiás (HV/UFG). The waste was classified in four groups (GA, GB, GD and GE) accordling to its nature: infectious, chemical, common and drill-sharp, respectively. The evaluation of the residues management was performed using a checklist prepared in accordance with actual legislation and after its validation and application in all sectors of the HV/UFG. The qualifying and quantifying were made through the inspection and weighing of these products. The study was carried out from may 04 to July 17, 2009. As results, it was observed that the management adopted in HV/UFG wasn t fully in accordance with the sanitary regime. Among the critical points observed, it can be outstand the inappropriate separation, identification and storage of the health services waste, poor training of the professionals involved, as well as, insufficient structural conditions. From the previously waste conditioned as GA, it was observed that only 28,5% were appropriately classified as GA. The rest, 2% was GB, 0.5% was GE and 69% was GD. It can be concluded that if the segregation was performed properly in accordance of sanitary legislation, could occur a reduction up to 71% of the contaminated residues. This result can be considered relevant because it implicate in considerable reduction in costs for the treatment of the health services waste, just as in the prevention of infectious diseases and occupational accidents during the handling of these residues.Item AVALIAÇÃO DO EFEITO DA INOCULAÇÃO DE Salmonella Enteritidis EM OVOS EMBRIONADOS E EM PERUS DE UM DIA(Universidade Federal de Goiás, 2008-08-28) ANDRADE, Carla Yoko Tanikawa de; CAFÉ, Marcos Barcellos; http://lattes.cnpq.br/9860968235125158; STRINGHINI, José Henrique; http://lattes.cnpq.br/8505634095383289; ANDRADE, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467This study was divided into two experiments, referring to incubation and early phase. The incubation experiment had as principal aims evaluated incubation yield, the capacity to penetrate through the eggshell and the ability to colonize the gastrointestinal tract. Four hundred fertilized turkey eggs for BUT9 strain were distributed in four treatments: CCS and CCM (inoculation of placebo in eggshell and air cell, respectively); ICS and ICM (inoculation with 4,2 X 104 UFC/mL of Salmonella enterica subspecies enterica sorovar Enteritidis in eggshell and air cell, respectively). Incubation parameters calculated were: fertility, total hatchability, hatchability of fertile eggs and poult yield. Salmonella was examined in eggshell, inner and outer membranes, albumen/yolk, embryo and, after hatched, in meconium in all poult. Qualitative results were analyzed by qui-square test. It was verified that the agent maintained feasible in eggshell during the hole incubation and migrated to egg components. Incubation parameters were not affected by the pathogen inoculated in eggshell. It was also observed that Salmonella Enteritidis inoculated in air cell determined early embryo mortality, while the control treatment influenced the mortality periods in relation with CCS. The intestinal colonization of Salmonella occurred in poults derived from eggshell experimental inoculation. In the second experiment, 135 poults derived from inoculation through eggshell and drinking water were evaluated by the effects of Salmonella Enteritidis to colonize the gastrointestinal tract, determine systemic infection, hurt the intestinal integrity and change the performance variables. At 10, 20 and 28 days, turkeys and feed were weighted to constitute calculation of performance variables. After weighage, five turkeys from each treatment were necropsied to obtain datas of weight and bacterial isolation of intestine, liver, spleen and heart. Qui-square test was employed to qualitative variables and the Anova procedure to quantitative variables and average compared by Tukey test (5%). The intestinal colonization increase significantly during early phase in treatment inoculate through eggshell, but none in treatment inoculated in drinking water. The intestine showed maximum development at 10 days and higher weight at first, 10th and 28th when Salmonella was present. The digestive and absorptive capacity was impaired in infected birds. Salmonella was capable of colonized gastrointestinal tract and established systemic infection. Mortality rate was high during first week of age, with less clinical signs or anatomopathological lesionsItem Contribuição ao estudo epidemiológico da Leishmaniose Visceral Canina por técnicas diretas e indiretas no município de Goiânia(Universidade Federal de Goiás, 2009-08-13) AZEVEDO, Elisa Maria Rennó; JAYME, Valéria de Sá; http://lattes.cnpq.br/0603234425928309; MOURA, Veridiana Maria Brianezi Dignani de; http://lattes.cnpq.br/8773201078957745; LINHARES, Guido Fontgalland Coelho; http://lattes.cnpq.br/6261928164195145Canine visceral leishmaniasis (CVL) is a chronic zoonosis caused by a protozoan called Leishmania (Leishmania) chagasi and has as vectors, the sandflies Lutzomyia longipalpis and Lutzomyia cruzi. The domestic dog is the most important reservoir in urban areas considering the zoonotic disease form and is responsible for maintaining the parasite in endemic foci. Aiming to assist the Superintendent of Policies for Comprehensive Health Care / Health State Department of Goiás (Spais / SES-GO) and the Department of Zoonosis Control from Goiânia (GO-DCZ) in epidemiological surveillance of the disease, this work investigated possible cases of CVL by using laboratory analysis. For this study, dogs seen at the Veterinary Hospital, School of Veterinary Medicine, Federal University of Goiás (HV / EV / UFG) with clinical suspicion of disease and symptomatic and asymptomatic dogs captured by DCZ-GO were analyzed. A total of 214 dogs were evaluated, and classified into three groups. Group 1 (GI): 33 dogs from DCZ with at least three clinical signs suggestive of LVC, Group 2 (GII): 141 clinically healthy dogs from DCZ and Group 3 (GIII): 40 dogs seen at the HV / EV / UFG with clinical suspicion of LVC. The frequency of animals classified as positive with different methods of diagnosis used in this study were: 5.6% (n = 12) in ELISA test, 9.35% (n = 20) in IFAT, 2.80% (n = 6) in the direct parasitological examination of skin imprints; 2.34% (n = 5) in direct parasitological examination of lymphonode, and 3.17% (n = 7) in PCR reactions of both the genderspecific protocol and the L. chagasi-specific. Considering the results and the conditions of the present study, it is concluded that there is casuistry in CVL series of symptomatic or asymptomatic dogs clinically attended in the city of Goiânia. The occurrence of Leishmania chagasi bearer dogs captured in the municipality of Goiânia were detected and the symptoms frequently observed in illness dogs carriers of L. chagasi in Goiânia are: conjunctivitis, lymphadenopathy, skin changes, cachexia and onychogryphosis.Item Ácidos orgânicos no controle de Salmonella Enteritidis em frangos de corte desafiados experimentalmente com Salmonella Enteritidis e Eimeria tenella(Universidade Federal de Goiás, 2009-03-27) CALAÇA, Gracinda Mariana; ANDRADE, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; LEANDRO, Nadja Susana Mogyca; http://lattes.cnpq.br/3136809931691012; CAFÉ, Marcos Barcellos; http://lattes.cnpq.br/9860968235125158This experiment was carried out using 504 one-day-old Hubbard female chicks, in order to evaluate the effect of a mixture of organic acids added to the ration, on the control of Salmonella in birds experimentally confronted with S. enteritidis and S. enteritidis associated with Eimeria tenella. The birds were distributed following a totally randomized sequence in factorial way 3x2, added or not the organic acid to the ration and the presence of challenge with S. enteritidis and E. tenella, isolated S. enteritidis or without challenge at all, totalizing six treatments with six repetitions each one, using 14 birds by experimental unit. The statistic data was analyzed using the program SAS, adopted to compare the averages, the Tukey test (quantitative variables) and Kruskal-Wallis (qualitative variables) to p< 0,05. The birds that received organic acid enriched ration had improvement in the performance at 7, 21 and 28 days. The birds inoculated only with S. enteritidis and the birds inoculated with S. Enteritidis and E. tenella presented worse levels of zootecnic performance (p<0,05) at 21, 28 and 35 days of life. The spleen weight and the thin intestine and cecum pH were not influenced (p>0,05) by the addition of organic acids to the ration. All the treatments challenged with S. enteritidis and which received acids presented minor frequency of bacteria isolation in the analyzed organs. It was also observed a reduction on the number of oocists of E. tenella in the excretes and in the cecum content in the treatments which had organic acids, as well as reduction of intestinal damage scores caused by infection with E. tenella. The organic acids promoted improvement on the zootecnic performance on cut chicken, improving intestinal health with positive consequences on control of S. enteritidis together with Eimeria tenellaItem Chlamydophila spp.,Mycoplasma gallisepticum E Mycoplasma synoviae em psitacídeos (filo: Cordata, ordem: Psittaciformes) de diferentes cativeiros no estado de Goiás(Universidade Federal de Goiás, 2012-02-29) CARVALHO, Andréa de Moraes; LINHARES, Guido Fontgalland Coelho; http://lattes.cnpq.br/6261928164195145; JAYME, Valéria de Sá; http://lattes.cnpq.br/0603234425928309; ANDRADE, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467The investigation of pathogens in wild animals can be considered an important tool for conservation of species and ecosystem. Chlamydiosis was first identified and is a common disease in psittacines. It is considered the most important zoonosis spread by these birds. Due to its infectious nature, this disease is studied all around the world. The mycoplasmosis is a common respiratory disease in pigeons, chickens and birds. However, parrots can be affected if they get in contact with the agent, and they can become a source of infection for the environment and for other birds. This study aimed to identify, through the polymerase chain reaction PCR technique, bacteria Chlamydophila spp., Mycoplasma gallisepticum and M. synoviae in captivity parrots in three different facilities in Goiás state: CETAS Centro de Triagem de AnimaisSilvestres, a conservation and a comercial captivity. The results were compared in order to determine wich facility is more perserved or contaminated by pathogens. For Chlamydophilaspp, CETAS facility showed 25.6% of positive samples, while the commercial captivity showed 26.4%, and the conservation captivity had no positive samples (0%). For the presence of Mycoplasma gallisepticum, CETAS presented 21.62% of positive samples, while the commercial and conservation captivity showed 15.7% and 14% of positive samples, respectively. M. synoviae was almost absent in CETAS (2.7%) and in conservation captivity (1.3%), and absent in commercial captivity (0%). The results found were compared using Fischer s exact test, where CETAS and commercial captivity were more infected than conservation captivity. The bacteria studied were present in different species of parrots, especially on the genera Ara, Aratinga and Amazonas. The presence of Chlamydophila and Mycoplasma sp. should be investigated in captive birds, since it reveals the presence of the agents in the population, allowing its control.Item Investigação epidemiológica de epizootia de febre amarela em primatas não-humanos em Goiás, no período de 2007 a 2010(Universidade Federal de Goiás, 2011-03-30) DELFINO, Denizard André de Abreu; JAYME, Valéria de Sá; http://lattes.cnpq.br/0603234425928309; SOUZA, Aires Manoel de; http://lattes.cnpq.br/2205630749435756; ANDRADE, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467The objective of this study was to carry out and analyze the actions taken to control the yellow fever epizootic in non-human primates, recorded in November 2007 to March 2010 in Aparecida de Goiania, Goias Brazil. The primary epidemiological information on the disease was obtained from the Municipal Health Secretariat, by the local Center for Zoonosis Control. Data were analyzed from the outbreak reports. In A descriptive study, the control actions, such as blocking vaccine against yellow fever by the municipal health department and the evaluation of physical barriers to contain the virus, were verified. The inspection of 58 dead nonhuman primates, representing 100% of the animals found in the period previously mentioned, was used. From these, 34.48% (20/58) were able to have samples collected, according to the specifications of the Epizooty Handbook Of this amount (58), 27.59% (16/58) of monkeys were collected and3.45% (2/58) presented positivity for yellow fever virus. By evaluating the actions that contributed to the epidemiological assessment at the city, it is emphasized the occurrence of three deaths among genera and species of nonhuman primates, Alouatta caraya, Cebus apella and Callithrix spp, with the circulation of yellow fever virus in two cities with epidemiological distinct characteristics. The areas which showed the highest incidence of deaths of monkeys had also the greatest degree of urban growth, characterized by recent environmental changes and residual maintenance of natural areas.Item Detecção de Enterobacteriaceae e Chlamydophila spp. em psitacídeos provenientes do centro de triagem de animais selvagens de Goiás(Universidade Federal de Goiás, 2010-02-26) HIDASI, Hilari Wanderley; JAYME, Valéria de Sá; http://lattes.cnpq.br/0603234425928309; LINHARES, Guido Fontgalland Coelho; http://lattes.cnpq.br/6261928164195145; ANDRADE, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467The wild animal trafficking is the third largest illegal activity in the world, ranking only behind the trafficking of weapons and drugs. The birds are the animals most affected by the illegal trade. Besides damaging to biodiversity, trafficking can also pose risks to human health. A number of diseases can be transmitted and acquired by the birds, the most commonly detected in the bacterial etiology. Improper management, especially related to transportation and overcrowding, promotes increased susceptibility to infections of birds or even the activation of latent infection with subsequent spread of pathogens. For these reasons this study aimed to conduct research and determine the Enterobacteriaceae resistance of strains of Escherichia coli, as well as raising the frequency of Chlamydophila spp. of parrots seized in actions to combat trafficking of wild animals in Goiás, with proper authorization from the Brazilian Institute of Environment and Renewable Natural Resources (IBAMA / GO). For this, 300 parrots in a period of one year were enrolled in the Screening Center of Wild Animals (CETAS), in Goiânia, Goiás, and underwent clinical and laboratory examinations. For Research Enterobacteriaceae were collected excreta from the bottom of cages, lined with aluminum foil and packed in ice for transport to the laboratory where they were examined by conventional bacteriological method. For the presence of Chlamydophila psittaci 300 cloacal swabs and 300 tracheids were collected and analyzed by polymerase chain reaction (PCR). We have identified the following excreta bacteria of the Enterobacteriaceae: Escherichia coli, 172/300 (33.87%), Enterobacter spp. 153/300 (30.12%), Klebsiella spp. 89/300 (17, 73%), Citrobacter spp. 9 / 300 (11.71%), Proteus vulgaris 21/300 (4.23%), Providencia alcalifaciens 5 / 300 (0.98%), Serratia sp.5/300 (0.98%), Hafnia aivei 3 / 300 ( 0.59%) and Salmonella sp. 1 / 300 (0.19%). Escherichia coli isolates were tested using the sensitivity to antibiotics, which revealed: amoxicillin (10mg) (70.93%), ampicillin (10mg) (75.58%), ciprofloxacin (5μg) (69.76%), chloramphenicol (30μg) (33.14% ), doxycycline (30μg) (64.53%), enrofloxacin (5μg) (41.28%), tetracycline (30μg) (69.19%), sulfonamide (300μg) (71.51%) of antimicrobial resistance. Of samples, 11/300 (3.66%) were positive in PCR analysis for Chlamydophilla spp. The results suggest that parrots from illegal trade are potential backers of zoonotic agents, and also point to the possibility that wild birds would constitute support for transfer of resistant phenotypes of E. coli to the human microbiota and other animalsItem Parâmetros hematológicos e bioquímicos em matrizes suínas com e sem infecção urinária tatadas com ácido cítrico e cloreto de amônio(Universidade Federal de Goiás, 2009-08-14) LOPES, Débora Vieira; SOBESTIANSKY, Jurij; http://lattes.cnpq.br/0995799988828810The urinary acidifiers as ammonium chloride and citric acid have been used in the treatment of urinary tract diseases. These illnesses lead to important losses to the brazilian and worldwide swine production. In the present work the hematological and biochemical parameters of sows getting citric acid and ammonium chloride in the food were evaluated. In the present study 48 animals were distributed into 6 groups: group sc-positive control, group nc-negative control, group sac-sows with urinary infection getting citric acid in the food, group sca-sows with urinary infection getting ammonium chloride in the food, nac-sows without urinary infection getting citric acid in the food and group ncasows without urinary infection getting chloride ammonium in the food. Significant differences were observed between the group of animals with urinary infection and the group of animals without urinary infection regarding the medium values of serum creatinine, hemoglobin, hematocrit and MCHC. The medium values of creatinine, hemoglobin and hematrocit were higher in the group of animals with urinary infection. The value of MCHC was higher in the group of animals without urinary infection. Regarding the different treatments, it was observed that the citric acid was able to increase the values of serum albumin in animals without urinary infection, to reduce the value of MCV and the white blood cells counting as well as it increased the value of MCHC in the group of animals with urinary infection and only the value of MCHC in this group was not according to the reference values. The ammonium chloride reduced significantly the values of hemoglobin and hematocrit in animals with and without urinary infection, as well as it reduced the MCV of animals with urinary infection, where the values were according to the reference values. The conclusion is that the presence of urinary infection changed the values of serum creatinine and that the use of citric acid and ammonium chloride didn t change significantly the hematological and biochemical parameters of sows with and without urinary infection.Item Estudo da etiologia das principais bactérias isoladas de mastite bovina em rebanhos leiteiros de propriedades rurais de Goiás(Universidade Federal de Goiás, 2012-03-02) MARTINS, Juliana Dias; MESQUITA, Albenones José de; http://lattes.cnpq.br/3180029815183858; OLIVEIRA, Antonio Nonato de; http://lattes.cnpq.br/8261502405023882; NICOLAU, Edmar Soares; http://lattes.cnpq.br/9601723963736071The bovine mastitis consists of an inflammation of the mammary gland, with acute to chronic evolution, which occurs when an infectious agent attacks the mammary gland. It is characterized by physical, chemical and sensory alterations of the milk, caused by microorganisms that invade the mammary gland and cause pathological changes in the glandular tissue. Mastitis can be classified according to the form of presentation as clinical or subclinical. Several symptoms are observed in the clinical form of the disease such as the secretion of milk with lumps, pus or watery aspect, and edemaciated stiff and febrile teats and udder. On the other hand, no symptoms can be observed in the mammary gland and milk in subclinical cases. Mastitis is a major cause of economic losses in dairy cattle farms, mainly due to reduced milk production, changes in the composition and increased somatic cell count (CCS). The objective of this study was to evaluate the prevalence of the main bacteria that cause (clinical and subclinical) bovine mastitis, and to check the sensitivity to various antimicrobial agents of clinical mastitis. The agents most frequently isolated from clinical mastitis were Streptococcus uberis and Staphylococcus aureus and the antibiogram demonstrated greater sensitivity and efficacy of the active principles linezolid, amikacin, gentamicin and imipenen regarding the isolated agents. In subclinical mastitis Streptococcus agalactiae, Streptococcus uberis and Staphylococcus sp. Were the agents most frequently identified and that determined the highest mean values of CCS. The average CCS of cows with subclinical mastitis was approximately 743 x 103 cs/mL. The study was conducted in eight dairy farms in the State of Goiás. The results were obtained by frequency analysis. In conclusion, that the results emphasize the importance of assessing the sensitivity profile of microorganisms that cause clinical mastitis in each herd studied allowing greater precision in medication prescription for future cases of clinical mastitis and for dry cow therapy. The use of other techniques such as real time PCR is also important for the detection of the main microorganisms causin subclinical mastitis, which can be indicated as a routine method, due to its specificity, providing a precise bacteriological diagnosis.Item Fonte de infecção e do perfil de resistência a antimicrobianos de Salmonella sp. isoladas de granjas de frango de corte(Universidade Federal de Goiás, 2010-03-26) MORAES, Dunya Mara Cardoso; NUNES, Iolanda Aparecida; http://lattes.cnpq.br/2555738917312914; JAYME, Valéria de Sá; http://lattes.cnpq.br/0603234425928309; ANDRADE, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467The objective of this research to investigate the presence of Salmonella sp. in raw materials of animal origin used in the manufacture of feed for broilers, in diets collected directly from bird feeders, and in organs ceca contents and liners carry case for newly hatched chicks, in environmental samples, in samples from swabs of the hands of officials of the farm and slaughterhouse samples and classify and determine the resistance of strains of Salmonella sp. found, before the action of chemotherapeutic nine. For data analysis was descriptive frequency results. 1200 samples were collected from flour and Salmonella sp. was found in 10.5% of samples with a predominance of serovar Enteritidis. The frequency of bacteria in meat meal was 12%, 6.8% in blood, the feathers of 4.3% and 14.6% in the viscera. Were also isolated Salmonella Cerro, Salmonella Montevideo, Salmonella anatum, Salmonella Tennessee, and Salmonella typhimurium among others. Regarding the resistance of strains found in the various categories of flour was observed resistance to sulfonamides, neomycin, tetracycline, sulphamethoxazole-to trimetopim and florfenicol. Of the three strains of bacteria isolated from two diets were of a Salmonella enteritidis and S. Anatum, showing resistance to sulfonamides and neomycin. Of the 32 batches of newly hatched chicks 9.4% were positive for Salmonella and 32 batches of liners carrying case 9.4%. Environmental samples, before bed accommodation, swabs of feeder and drinker and the drinking water of birds tested negative for Salmonella sp .. Drag swabs of poultry manure, and samples Alphitobius diaperinus swabs from the hands of officials of the farms had a frequency of 12.5%, 12.5% and 6.5% respectively. In samples from swabs of drag serovar Enteritidis was the most frequent and catfishes of the samples and swabs of hands was the only serovar isolated. In samples from slaughterhouse 26.7% of the lots from crop and 33.3% of batches of ceca were positive for Salmonella sp .. Regarding the resistance of strains, there was resistance sulfonamides, the amoxicillin and enrofloxacin in samples of newly hatched chicks and amoxicillin in the samples of liners carrying case. In drag swabs and samples of catfishes, the bacteria were resistant to sulfonamides. In samples from crop, were resistant to sulfonamide and enrofloxacin and the caeca to sulfonamides, the trimetopim-sulphamethoxazole, tetracycline, amoxicillin and ampicillin to.Item Aspectos epidemiológicos da leishmaniose visceral canina nas regiões administrativas Lago Norte e Sobradinho II do Distrito Federal - DF(Universidade Federal de Goiás, 2011-08-31) NASCIMENTO, Geovani San Miguel; JAYME, Valéria de Sá; http://lattes.cnpq.br/0603234425928309; ANDRADE, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467This study aimed to determine the prevalence and epidemiological aspects of canine visceral leishmaniasis in Lago Norte and Sobradinho II, administrative regions of the Federal District. Census surveys were conducted in four localities of the Federal District where the data were collected and blood sampled from 5,551 dogs in Lago Norte and from 272 dogs in Varjão and from 836 dogs from Bananal and Engenho Velho communities in Sobradinho II. We evaluated age, sex, breed, presence of clinical signs, wooded homes, the presence of the garden, organic material, home situation and the presence of chicken in the properties. The sera samples were submitted to indirect immunofluorescence test (IFA), and samples with reagent titers greater than or equal to 1:40 were considered positive. Prevalences of 6.66% (17/255), 13.13% (44/335), 15.40% (61/396) and 22.32% (1013/4538), were found in Varjão, Engenho Velho, Bananal and Lago Norte. Regarding age, 10.90% (70/643) were aged up to one year, considered a young population, 22.40% (619/2763) were at one to six years of age, considered as a middle-aged population, and 24.00% (297/1236) were older than six years of age, corresponding to old animals. Regarding the presence or absence of clinical signs, 30.31% (238/785) had some clinical changes and 18.61% (846/4544) showed no clinical changes. One can conclude that this canine visceral leishmanias is distributed in different regions of the Federal District being the location, the age and the presence of clinical changes the highest risk factors The knowledge gained on the prevalence of leishmaniasis in dogs will allow the establishment of effective strategies for control actions.Item Aspectos físico-químicos e microbiológicos da urina, pH e consistência das fezes de matrizes suínas suplementadas com ácido cítrico e cloreto de amônio(Universidade Federal de Goiás, 2010-02-19) OLIVEIRA, Fábio Henrique de; SOBESTIANSKY, Jurij; http://lattes.cnpq.br/0995799988828810; STRINGHINI, José Henrique; http://lattes.cnpq.br/8505634095383289; NUNES, Romão da Cunha; http://lattes.cnpq.br/4335133347523404Urinary infection is a multifactorial disease, very common in swine herds worldwide, is considered a major cause of reproductive failure that adversely affect the productivity levels of swine. In control of this infection has been frequently used acidifiers the urine. The present study was to evaluate the action of two acidifying, citric acid and ammonium chloride, used to control urinary tract infection, on the physico-chemical and microbiological tests of urine, pH, color and consistency of the feces of swine females in production. We used 48 pregnant females divided into six groups: control (-): females without urinary infection; control (+): females with infection, acid (-): females without infection receiving citric acid, acid (+): infected females receiving citric acid, chloride (-): females without infection receiving ammonium chloride, chloride (+): females with infection receiving ammonium chloride. Were collected samples of urine and feces on days zero, seven and 15 of treatment to perform on the physical-chemical and microbiological analysis. The urinary variables studied were: color, odor, pH, density, protein, sediment and bacterial count. The feces samples were evaluated for pH, color and consistency. The results showed that ammonium chloride was able to reduce the urinary pH, but did not affect the other urinary parameters. Since citric acid showed effect on the color of the urine with a tendency to yellow. The citric acid reduced the bacteriology count. The two acidifiers didn t can reduce the bacterial count, demonstrating that they did not have a bactericidal or bacteriostatic action. The pH of feces increased after supplementation with citric acid and ammonium chlorideItem ASPECTOS EPIDEMIOLÓGICOS DA INFECÇÃO POR LEPTOSPIRA SPP. EM FELINOS DOMÉSTICOS (FELIS CATUS) APARENTEMENTE SADIOS DA REGIÃO METROPOLITANA DE GOIÂNIA , GOIÁS(Universidade Federal de Goiás, 2009-03-26) PARREIRA, Ivonete Maria; JAYME, Valéria de Sá; http://lattes.cnpq.br/0603234425928309In felines, the leptospirosis is described commonly as of rare occurrence, in spite of inquiries sorologic accomplished at several countries they have already registered positive reactions in these you encourage. In this research it was aimed at to evaluate the presence of antibodies anti-Leptospira spp in samples of serums of domestic felines originating from of the areas of Goiânia and of Aparecida from Goiânia, Goiás, Brazil, besides evaluating biochemical parameters and of investigating decisive factors associated to the infection. Sanguine serums of 330 domestic felines were analyzed seemingly healthy, being 113 of domiciled animals in different neighborhoods of Goiânia and 217 of individuals picked up to the Center of Zoonosis of Control (CCZ) of Aparecida from Goiânia. The research of antibodies anti-Leptospira spp. it was accomplished through the test of microscopic agglutination (SAM) with alive antigens. Parallel, dosagens were proceeded of range-glutamiltransferase (GGT), alkaline phosphatase (FA), urea and creatinine. The presence of agglutinative antibodies was detected in 23 animals (6,96%), with titles that varied from 1:100 to 1:800, with predominance of co-agglutinations (7,76%) and of the sorovars Cynopteri and Djasiman. In Goiânia they were identified 10 animals (8,84%) positive seruns, while in Aparecida from Goiânia 13 were detected (5,99%) positive seruns. Reactions were verified for the sorovars Australis, Bratislava, Butembo, Castellonis, Canicola, Cynopteri, Djasiman, Hebdomadis, Copenhageni, Icterohaemorrhagiae, Pomona, Pyrogenes, Hardjo, Patoc, with minimum title of 1:100. significant difference was not observed by the test of regression logistics, between the positive and biochemical alterations as well as between positive and age and sex of the animals. Biochemical alterations and age came related. The obtained results served as parameters for the evaluation of the infection in domestic felines in the study area, contributing to the elucidation of epidemic aspects and for the control of the zoonosis, seeking to reduce the risk to the human health and your impacts sanitary, social and economical.Item Ectoparasitofauna do híbrido tambacu (Colossoma macropomum x Piaractus mesopotamicus) oriundos de pisciculturas da microrregião de Goiânia-GO(Universidade Federal de Goiás, 2011-09-29) PEREIRA, Fabricio de Oliveira; SILVA, Paulo César; http://lattes.cnpq.br/4162443606090063; ROMERO, Rolando Alfredo Mazzoni; http://lattes.cnpq.br/0737991281916166; LINHARES, Guido Fontgalland Coelho; http://lattes.cnpq.br/6261928164195145Fish farming has many commercial losses due to diseases. Parasitic diseases account for a large portion of this losses. In Brazil there are records of various parasites causing damage, and in Goiás were not found recent data on the occurrence of parasitic infections in commercial fish farms. This study aimed to identify and establish the occurrence of ectoparasites of tambacu hybrid, raised in commercial fish farms in the microregion of Goiânia-GO. Nine fish farms were evaluated between August and December of 2010, and samples were colected in two separate visits. Fishs were examined by direct microscopy for observation of the parasites. Of the 90 tambacu fish examined 46 (51.11%) were parasitized. It is concluded that hybrid tambacu fishes raised in the Microregion of Goiânia has the following parasites: Monognea sub-class, Trichodina sp.. Piscinoodinium pillulare, copepodids of Lernaea sp. and Dolops sp.Item Avaliação de parametros clínicos, hematológicos e viabilidade econômica do uso do florfenicol na metafilaxia das afecções respiratórias inespecíficas de bovinos confinados no estado de Goiás(Universidade Federal de Goiás, 2010-11-30) REZENDE, Marcus Luciano Guimaraes; BORGES, Jose Renato Junqueira; http://lattes.cnpq.br/3586382943482522; VIANA, Rinaldo Batista; http://lattes.cnpq.br/7919022855581617; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257Producing more in less physical space and in less time, increasing the profitability and reducing sanitary and economical risks are some of the challenges of the Brazilian livestock that the intensive breeding aims to supplant. A considerable increase in the number of confining unities as well as in feedlot cattle is already observed, thus, the attention to the control of pathological alterations deriving from the production intensification that can compromise the profitability and the economical viability of such activities should also increase. Considering the context presented herein, this essay aimed to analyze the economical/sanitary viability of the usage of florfenicol (40 mg/kg) in the metaphylaxis of bovine respiratory diseases (BRD) in feedlot cattle in the state of Goiás, where two groups totalizing 125 animals were concurrently monitored during 100 days. The first group, having 32 animals, was maintained under the standard sanitary protocol of the confining groups and it was called control (G1). The second group, having 93 animals, was submitted to metaphylaxis with florfenicol (40 mg/kg) at the beginning of the confinement and it was called metaphylaxis (G2). In the both groups, the sanitary and the productive standards were evaluated and a financial analysis was performed in order to economically validate the method. The animals were examined, weighted and materials for analyses were collected at the beginning and at the end of the confinement, when the occurrence of respiratory problems in the animals was evaluated. The animals submitted to metaphylaxis with florfenicol had a daily weight gain (DWG) superior to the one of the animals pertaining to the control group, and the indexes of BRD demonstrated through the analysis of the method viability (MV) were superior to 2.7%, which justifies the investment in the prevention of BRD.Item Identificação de herpesvírus bovino em amostras de cérebro(Universidade Federal de Goiás, 2011-02-24) SILVA, Duanne Alves da; BRITO, Luiz Augusto Batista; http://lattes.cnpq.br/3212353728321020; BRITO, Wilia Marta Elsner Diederichsen de; http://lattes.cnpq.br/7605775995731168Herpetic meningoencephalitis is an infection of the central nervous system caused by bovine herpesvirus 5 (BoHV-5) despite BoHV-1 also being associated with this pathology. The course of the disease is usually fatal. Meningoencephalitis has caused significant economic burden to the Brazilian cattle industry due to its high mortality rate, especially in young animals. Because of cross-reactivity and the absence of an effective serological test that differentiates types 1 and 5 of herpesvirus, the actual prevalence of infection with both viruses is unknown. It is estimated that some animals, supposedly infected with type 1, may be seropositive for type 5. For a better understanding of the molecular epidemiology of this virus in Goiás, 110 brain samples of young cattle, that died with neurological signs, referred to the reference laboratory of the State of Goiás for rabies diagnosis in 2008, were analyzed by multiplex PCR to amplify the glycoprotein C (gC) region of the BoHV-1 and -5 DNA. Of the 110 samples, 53.6% were positive for bovine herpesvirus. Of these, 22.0% were positive for BoHV-1, whereas 52.5% were positive for BoHV-5. Furthermore, 13.6% of samples showed co-infection for types 1 and 5. Among these, one sample came from a buffalo. Positivity for both bovine herpesvirus and rabies virus was observed in 53.3% of samples. This study showed that BoHV-1 and -5 are circulating among cattle in the State of Goiás and that herpesvirus type 1 can also be encephalitogenic. Although there was no evidence of viral replication, after inoculation of all 20 brain samples in cell culture, the possibility of viruses being associated with neurological disease cannot be ruled out. This was the first study to demonstrate the presence of BoHV-1 and BoHV-5 in samples obtained from herds in Goiás.Item Controle de Salmonella Typhimurium em frangos de corte utilizando lactose(Universidade Federal de Goiás, 2009-07-25) SOUZA, Eliete Silva e; STRINGHINI, José Henrique; http://lattes.cnpq.br/8505634095383289; CAFÉ, Marcos Barcellos; http://lattes.cnpq.br/9860968235125158; ANDRADE, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467This study was conducted at the Experimental Center of Poultry Diseases and Laboratory of Bacteriology Department of Veterinary Medicine Veterinary School, Federal University of Goias to evaluate the effects of lactulose in Salmonella typhimurium in broilers. The experimental design was completely randomized, evaluating substance applied (lactulose) and bacterial challenge. We used 630 chicks of commercial strain, which were distributed in six treatments with seven replicates and 15 birds per experimental unit. The microbial challenge was provided to birds in the first days of life containing 5.0 X 102 CFU / 0.5 mL of Salmonella typhimurium. And lactulose at a dose of 0.023 mL / g of body weight up to 14 days of age. The weights of the birds and feed intake were seen weekly for performance measurement. On days seven, 14, 21 and 28, one bird per treatment was necropsied, and liver collected for biometric analysis and histopathological. The contents of ingluvie and caeca were submitted to enumeration of Salmonella and Escherichia coli and bacterial analysis, as well as the spleen, liver and heart were examined bacteriologically. Was also measured the pH of ingluvie, small intestine and cecum and blood collected for evaluation of liver enzymes. The quantitative performance data, biometrics liver, pH, bacterial count and liver enzymes were analyzed by applying ANOVA and Tukey (5%), and data resulting from analysis of fecal excretion of Salmonella were analyzed using the nonparametric chi-square . There was greater weight gain and lower feed conversion (P <0.05) in groups receiving lactulose with or without S. Typhimurium to 21 days of life and decreased fecal excretion of S. Typhimurium at 10 days of age for birds fed lactulose (P <0.05) from the first days of life. It was also found that the lactulose caused decrease in values (P <0.05) pH in the organs of the digestive system for seven days of life and that lactulose decreased (P <0.05) the CFU of E. coli in ingluvie at 21 and 28 days and S. Typhimurium in the treatments administered lactulose before the pathogen and ages. Lactulose prevented infection in the early period of life for birds, showing a better effect when administered 48 hours before inoculation. It was also that there was an increase in relative liver weight by S. Typhimurium in all ages studied and that the lactulose caused mild liver abnormalities, and it was also found that the S. Typhimurium did not cause severe clinical signs or mortality and that its recovery rate decreased with age. It can be concluded that lactulose causes greater weight gain in birds until one week after delivery, as well, was able to decrease the fecal excretion of S. Typhimurium. It also appears that the lactulose changed the pH values, except pH and reduced cecal colonization of S. Typhimurium in the cecum and the number of CFU of E. coli in ingluvie. It is also concluded that lactulose was able to prevent systemic infection while being provided, showing better preventive effect, and also ordered discrete liver, a process similar to congestive