Análise da resposta imune celular de pacientes com tuberculose pulmonar ativa contra os antígenos recombinantes MPT-51, GLcB, ESAT-6, Ag 85A e a proteína do filtrado de cultura (CFP) de mycobacterium tuberculosis
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Data
2008-02-21
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Universidade Federal de Goiás
Resumo
This work characterized the specific cellular immune response of TCD4 and
TCD8 lymphocytes against recombinant Mycobacterium tuberculosis at the
Hospital Anuar Auad, Goiania Brazil, and constituted of two experimental
groups: 1) 22 active TB patients with positive acid fast aputum, X-ray indicative
of tuberculosis, smear culture positive for M. tuberculosis and HIV negative. 2)
15 sex and age matched healthy controls, tuberculin skin test and HIV negative.
Venous blood was drawn and processed to obtain PBMC that were cultivated
for 96 hours with the specific antigens (1mg/106 cells). TCD8 and TCD4 cells
were analyzed by flow citometry for IL-10 and IFN-g production. In general, the
percentage of positive TCD4 and TCD8 cells for IFN-g and IL-10 were superior
among the TB patients. Additionally, TCD4+IFNg+ (5,63±2,43) and IL-10+ (5,83±
2,19) cells were significantly higher in TB patients than in healthy controls
(TCD4+IFNg+ =1,75±0,71 and IL-10+ =1,47±0,90), (p<0,01). Regarding the
percentage of TCD8 cells, a higher percentage of IFNg+ (4,33±1,45) and IL-10+
(4,01±1,14) among TB patients than controls (TCD8+IFNg+ = 1,49±0,42 and IL-
10+ 1,62±0,59) was observed (p<0,01). TB treatment did not alter the response to the tested antigens immediately after the treatment initiation. In conclusion,
the recombinant antigens MPT-51, ESAT-6, GLcB, Ag85A were recognized by
the specific immune response of active TB patients.
Key words: Tuberculosis, Mycobacterium tuberculosis, Recombinant antigens,
Cellular immune response.
to the tested antigens immediately after the treatment initiation. In conclusion,
the recombinant antigens MPT-51, ESAT-6, GLcB, Ag85A were recognized by
the specific immune response of active TB patients.
Descrição
Citação
VASCONCELOS JUNIOR, Arioldo Carvalho. Analysis of Cellular Immune Response Against MPT-51, GLcB, ESAT-6, Ag 85A Recombinant Antigens and the Culture Filtered Protein (CFP) from Mycobacterium tuberculosis from Patients with Active Pulmonary Tuberculosis. 2008. 137 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2008.