Envolvimento de células ER-MP58+ na produção de IL-12 em linfonodos drenantes da infecção inicial por leishmania major em camundongos BALB/c
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2006
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Universidade Federal de Goiás
Resumo
The development of a Th1 immune response is the key event to prevent Leishmania
infection and is linked with the IL-12 production by monocytes, dendritic cells,
macrophages and neutrophils. The IL-12 signal induces the increase of IFN-γ
production by T cells, favoring the profile of the Th1 immune response and the
resistance phenotype to the infection. In the vertebrate host, the leishmania inhabits in
cells of the mononuclear phagocyte system (MPS), which is also responsible for the
elimination of the parasite. The participation of immature cells of MPS in the initial
production of IL-12 has been discussed. It was demonstrated recently that a
population of mononuclear phagocytes, derived from bone marrow culture and
expressing the marker ER-HR3 produced great amount of IL-12p40 after stimulation in
vitro with procyclic or metacyclic promastigotes of L. (L.) major. The initial production
of IL-12 by MPS cells is under investigation. It is unclear the role of mononuclear
phagocytes, in different stages of maturation, in the production of
IL-12p40 in vivo. In this work, we evaluated the involvement of MPS cells, in different
stage of maturation, with the IL-12p40 production in vivo in draining lymph nodes of
BALB/c mice after 48 h of infection with L. (L.) major. Our results showed that CD31+
cells (mononuclear phagocytes precursors) were absent in the draining lymph nodes
of the subcutaneously footpad injection and that the number of ER-MP58+
(immature mononuclear phagocytes), ER-HR3+ cells (mononuclear phagocytes in the intermediate stage of maturation) and 33D1+
(mature phagocytes) increase in the period of 48 h after infection. We showed, also, that ER-MP58+
responsible for great part of the draining lymph nodes IL-12 production 48 h after
infection with stationary-phase promastigotes of L. (L.) major in mice BALB/c. We
suggested that, in vivo, the ER-MP58+ population, together with other cellular
populations, known as IL-12 producers, ER-MP58+ cell has an important role in the inflammation induced by the parasitic inoculum.
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CARDOSO, Ludimila Paula Vaz. Envolvimento de células ER-MP58+ na produção de IL-12 em linfonodos drenantes da infecção inicial por Leishmania major em camundongos BALB/c. 2006. 77 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2006.