Caracterização fenotípica e molecular de Xanthomonas axonopodis pv. phaseoli e Xanthomonas fuscans subsp. fuscans procedentes de regiões produtoras de feijoeiro-comum no Brasil
Nenhuma Miniatura disponível
Data
2014-02-25
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
The Common bacterial blight caused by Xanthomonas axonopodis pv. phaseoli (Xap) and
Xanthomonas fuscans subsp. fuscans (Xff), is one of most important bacterial etiology diseases from
the common bean plant. This pathogen causes a great loss in the production, mainly in the “water”
harvest, when the environmental conditions are helpful to the development of this disease and to its
dissemination. Among the strategies about integrated handling of diseases, the genetic resistance is the
main measure of control and, for obtaining some cultivate with lasting resistance and of notorious
specter, it is important to the know the genetic pathogen diversity. The present work has the goal of
studying the diversity and the genetic structure around and inside the population of Xap and Xff using
the markers rep-PCR; to connect the genetic pathogen diversity with its geographical distribution inside
Brazilian producing common bean plant regions. There were obtained 42 Xap and Xff isolated and four
Xanthomonas isolated not pathogens of to the bean plant, originated in the States of São Paulo, Goiás,
Paraná and Rio Grande do Sul. The genetic profiles obtained by primers BOX, ERIC and REP
produced 12 haplotipes matched (HC), where the HC 3, specific xff, was more frequent in PR and GO,
and was not found in RS. The dendrogram produced through the analysis showed that Xap, Xff and
isolated not pathogens are genetically different. In the analysis of genetic structure, the total genetic
diversity (Ht), among three populations was 0,2385 revealing that there was variability from a
population to the other one. Higher values of the Shannon rate (0,3648) show that Xap population is
more different genetically. From a total of 51 locos, 94,12 % are polymorphic, inside Xap population
they are all polymorphicand inside Xff, only 18,18 % are polymorphic. The genetic differentiation
coefficient (Gst = 0,5194) revealed diversity inside and among the patogenic populations, confirming
AMOVA’s result, where 51,98 % of the diversity was among the populations and 48,06 % inside them.
Then, the technique helped how to find differences between Xap and Xff and measure the values of
genetic diversity among and inside populations. The results of this study give us much information
about the pathogens diversity, which is considered useful to identify and to characterize the resistant
germoplasm in a more efficient way.
Descrição
Palavras-chave
Citação
PAIVA, B. A. R. Caracterização fenotípica e molecular de Xanthomonas axonopodis pv. phaseoli e Xanthomonas fuscans subsp. fuscans procedentes de regiões produtoras de feijoeiro-comum no Brasil. 2014. 67 f. Dissertação (Mestrado em Genética e Melhoramento de Plantas) - Universidade Federal de Goiás, Goiânia, 2014.