Avaliação bioquímica in vitro do metabolismo energético de cisticercos de Taenia crassiceps expostos a um derivado benzimidazólico, RCB20
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2015-02-23
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Universidade Federal de Goiás
Resumo
Human infections caused by Taenia spp may be more frequent than they are reported
especially if occurred in immunodepressed patients and if the location of the parasites does
not involve vital organs. Albendazole is one of the drugs used in the treatment of such
infections and its use in mass treatment campaigns may accelerate the resistance
development from the parasites both in humans and animal hosts. The aim of this study was
to in vitro evaluate the biochemical alterations in T. crassiceps cysticerci after the treatment
with a benzimidazolic derivatice (RCB20). The cysticerci were removed from the peritoneal
cavity of BALB/c mice with 30 days of infection, macroscopically classified and harvested into
6 well culture plates. 30 cysticerci from each development stage in each well received 5mL of
supplemented RPMI culture medium which contained sulfoxide albendazole (ABZSO) (6,5 μM
or 13 μM), or RCB20 (6,5 μM or 13 μM). After 24h of culture the cysticerci were removed from
the culture medium and both were frozen in liquid nitrogen. Liquid chromatography of high
performance and spectrophotometric analysis were performed. It was possible to observe a
difficulty in the secretion of substances by the treated cysticerci due to differences in the
concentrations of the products dosed from the vesicular fluid and from the culture medium.
This was an effect from the mode of action of the drugs which affects the tubulin formation.
Regarding the glycolysis it was possible to observe a gradation increase in the
secretion/excretion (S/E) of lactate in the treated groups while there was a decrease of this
organic acid in the vesicular fluid. As to the energetic metabolism and the citric acid cycle it
was possible to observe its traditional sense functioning due to the detection of alf-
ketoglutarate. It was possible an increase in the concentrations of citrate, malate, fumarate
and succinate in the treated groups. The alternative pathway of energy production such as
beta-oxidation of fatty acids and protein catabolism were detected with an increase in the
metabolites from those pathways in the treated groups. Therefore we conclude that in spite
of not blocking the glucose uptake the drugs influenced its uptake which lead to the use of
alternative pathways of energy production. In spite of more soluble and stable the RCB20
formulation did not present a different biochemical effect than ABZSO.
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FRAGA, Carolina Miguel. Avaliação bioquímica in vitro do metabolismo energético de cisticercos de Taenia crassiceps expostos a um derivado benzimidazólico, RCB20. 2015. 75 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2015.